Prepared Staining Kits for Histology and Pathology
A.1 BACTERIAL STAINS

Truant's Fluorescent Method for Acid Fast Organism
For bacterial stain.
Stain results:
Acid Fast: |
|
Fluoresce reddish yellow |
Artifacts: |
|
Fluoresce yellow |
References:
- Truant, J.P. Henry Ford Hospital Med. Bull., 10:287, 1962.
- AFIP Manual of Histological Staining Methods, 3rd ed. Ed. L. Luna: New York: McGraw Hill Publications. c. 1968. p. 219.
- Clark, G.: Staining Procedures. Williams and Wilkens Co., Baltimore. 3rd edition. c.1973. p. 225
RT |
26102-1A |
Weigert's Iron Hematoxylin Solution A |
 |
500 ml |
41.00 |
Add to Cart |
RT |
26102-1B |
Weigert's Iron Hematoxylin Solution B |
 |
500 ml |
27.00 |
Add to Cart |
RT |
26102-02 |
Auramine-Rhodamine Staining Solution |
|
250 ml |
50.00 |
Add to Cart |
RT |
26102-03 |
Acid Alcohol 1% |
 |
500 ml |
21.00 |
Add to Cart |

Brown and Brenn Method for Gram Positive and Gram Negative Bacteria
For bacteria stain: Gram + / Gram – bacteria and filaments of nocardia, etc.
Stain results:
Gram + Bacteria, Nocardia and Actinomyces Filaments: |
|
Blue |
Gram – Bacteria, Nuclei: |
|
Red |
Additional tissue elements: |
|
Yellow |
Note: Also see the Taylor Modification of the Brown and Brenn +/- technique noted for the varying differentiation available. Over-differentiation in the B&B sep 6 is a problem with some sections; run the control slides at varying rates to determine the amount for the specific organism.
References:
- Brown J.H. and Brenn, L. Bull. Johns Hopkins Hosp., 48:69 (1931)
- AFIP Manual of Histologic Staining Techniques: 3rd. ed., ed. G. Luna; New York: McGraw-Hill Publications, c. 1968, p. 222
RT |
26105-01 |
Crystal Violet Staining Solution, 1% Aqueous |
 |
500 ml |
25.00 |
Add to Cart |
RT |
26105-02 |
Sodium Bicarbonate, 5% Aqueous |
 |
500 ml |
23.00 |
Add to Cart |
RT |
26105-03 |
Basic Fuchsin, 0.25%, Aqueous |
 |
250 ml |
22.00 |
Add to Cart |
RT |
26105-3A |
Basic Fuchsin Working Solution |
 |
250 ml |
13.00 |
Add to Cart |
RT |
26105-04 |
Gram's Iodine Solution |
 |
500 ml |
22.00 |
Add to Cart |
RT |
26105-5A |
Acetone: Alcohol, 1:1 |
 |
500 ml |
16.00 |
Add to Cart |
RT |
26105-06 |
Picric Acid-Acetone Solution, 0.1% |
 |
500 ml |
38.00 |
Add to Cart |
RT |
26105-07 |
Acetone |
 |
1 qt |
15.00 |
Add to Cart |
RT |
26105-08 |
Acetone: Xylene |
 |
500 ml |
21.00 |
Add to Cart |

Brown-Hopps Method for Gram
Positive and Gram Negative Bacteria
For bacterial stain.
Stain results:
Gram Negative Bacteria: |
|
Red |
Gram Positive Bacteria: |
|
Blue |
Background: |
|
Yellow |
References:
- AFIP Manual of Histologic Staining Methods; ed. G. Luna, McGraw-Hill Publications, 3rd. ed.; New York, c. 1968, p.224
- Brown, R.C. and Hopps, H.C., Geographic Pathology Division Armed Forces Institute of Pathology, Washington, D.C. 20305
RT |
26106-01 |
Crystal Violet, 1% Aqueous |
 |
500 ml |
24.00 |
Add to Cart |
RT |
26106-02 |
Gram Iodine Solution |
 |
500 ml |
22.00 |
Add to Cart |
RT |
26106-03 |
Basic Fuchsin, 0.5% Aqueous |
 |
500 ml |
32.00 |
Add to Cart |
RT |
26106-04 |
Gallego's Differentiating Solution |
 |
500 ml |
19.00 |
Add to Cart |
RT |
26106-05 |
Cellosolve |
 |
500 ml |
31.00 |
Add to Cart |
RT |
26106-06 |
Tartrazine, 1.5% Aqueous |
 |
500 ml |
24.00 |
Add to Cart |

Taylor's Method for Gram Positive and Gram Negative Bacteria
For bacterial stain.
Stain results:
Gram-Positive Organism: |
|
Blue to Blue-Black |
Gram Negative Organism: |
|
Bright Red |
Nuclei: |
|
.Brownish Red |
Erythrocytes: |
|
Red to Yellow-Green |
Necrotic Tissue: |
|
.Yellow-Green |
Cytoplasm: |
|
Yellow |
Connective Tissue: |
|
Red |
References:
- AFIP Manual of Histological Staining Methods, 3rd ed., Ed. L. Luna: New York: McGraw Hill Publications, c. 1968, p.226
- Clark, G.: Staining Procedures, Williams and Wilkins Company, Baltimore, 3rd Ed, c.1973, p.320
- Taylor, R.D., Amer. J. Clin. Path. 46:472 (1966)
RT |
26108-01 |
Harris Hematoxylin |
 |
500 ml |
31.00 |
Add to Cart |
RT |
26108-02 |
Crystal Violet, Hucker's |
 |
500 ml |
25.00 |
Add to Cart |
RT |
26108-03 |
Basic Fuchsin Stock Sol. 0.1% |
 |
500 ml |
32.00 |
Add to Cart |
RT |
26108-04 |
Gram's Iodine Solution |
 |
500 ml |
22.00 |
Add to Cart |
RT |
26108-05 |
Lithium Carbonate Solution (Saturated, aqueous) |
 |
500 ml |
24.00 |
Add to Cart |
RT |
26108-06 |
Acid Alcohol, 1% |
 |
500 ml |
20.00 |
Add to Cart |
RT |
26108-07 |
Acetone : Alcohol, 1:1 |
 |
500 ml |
16.00 |
Add to Cart |
RT |
26108-08 |
Acetone |
 |
500 ml |
10.00 |
Add to Cart |
RT |
26108-09 |
Picric Acid-Acetone Sol., 0.1% |
 |
500 ml |
36.00 |
Add to Cart |
RT |
26108-10 |
Acetone-Xylene I |
 |
500 ml |
34.00 |
Add to Cart |
RT |
26108-11 |
Acetone-Xylene II |
 |
500 ml |
35.00 |
Add to Cart |

Gridley's Method for Endamoeba Histolytica
For bacteria stain: Amoebae, their nuclei and ingested erythrocytes
Stain results:
Erythrocytes (ingested): |
|
Rose |
Connective tissue: |
|
Green |
Amoebae: |
|
Blue-green |
Nuclei (amoebic): |
|
Darker blue-green |
References:
- Gridley, M.F.: Am. J. Clin. Pathology, 24:243 (1954)
- Luna, L.G.: Histologic Staining Methods, 3rd ed.: New York: McGraw-Hill Book Co., c.1968, p. 228
RT |
26109-01 |
Harris Hematoxylin, OR |
|
500 ml |
34.00 |
Add to Cart |
RT |
26109-1A |
Weigert's Iron Hematoxylin Solution A, AND |
 |
500 ml |
36.00 |
Add to Cart |
RT |
26109-1B |
Weigert's Iron Hematoxylin Solution B |
 |
500 ml |
26.00 |
Add to Cart |
RT |
26109-02 |
Aniline-Eosin Solution (Poison Pack) |
 |
250 ml |
30.00 |
Add to Cart |
RT |
26109-03 |
Naphtol Green B Staining Solution, 1% |
 |
250 ml |
13.00 |
Add to Cart |
RT |
26109-04 |
Acid Alcohol, 1% in 75% Alcohol Solution |
 |
500 ml |
18.00 |
Add to Cart |
RT |
26109-05 |
Ammonia Water, 0.3% |
 |
500 ml |
14.00 |
Add to Cart |

Giemsa for Helicobactor Pylori and Mast Cells
For bacterial, fungal stains.
Stain results
Red Blood Cells: |
|
Pink |
Nuclei: |
|
Blue |
H-Pylori, fungi & other bacteria: |
|
Blue |
Mast Cells: |
|
Purple |
Rickettsia: |
|
Purple |
References:
- Helicobactor pylori in peptic ulcer disease, NIH Consensus Statement Online, January 1994, 12(1): 1:23
- Luna 1AFIP 3rd ed. McGraw Hill, 236
- Fail, Rena, AS, HT (ASCP) Medical University of South Carolina, Histologic. Vol. XXXIV, No 2, Nov. 2001, p. 32.

May-Grunwald Giemsa for H-Pylori
For bacterial, fungal stain.
Stain results:
H-pylori: |
|
Blue |
Background: |
|
Light purple |
References:
- Carson, F. Histotechnology: A Self-Instructional Text. 1980-109.
RT |
26114-01 |
Jenner's Stain Stock Solution |
 |
250 ml |
20.00 |
Add to Cart |
RT |
26114-02 |
Giemsa Stock Solution |
 |
100 ml |
17.00 |
Add to Cart |
RT |
26114-03 |
Acetic Acid, 0.1% Solution |
 |
500 ml |
17.00 |
Add to Cart |
Genta, Robason and Graham Stain for H-pylori & Gastric Morphology
Stain results:
Muscle cells: |
|
Brownish yellow |
Lamina propria ground substance: |
|
Light yellowish or grayish |
Smooth muscle fibers: |
|
Light Pink |
Cystoplasm of neutrophils: |
|
Pale Pink |
Eosinophils: |
|
Intense red |
Nucleus: |
|
.Dark brown |
Intestinal metaplasma: |
|
Bright blue |
References:
- Genta PM., Robason GO, Graham DY-Simultaneous Visualization of Helicobactor Pylon and Gastric Morphology A New Stain Hum Pathol. 25:22 1-226, 1994.
RT |
26116-01 |
Uranium Nitrate, 1% Aqueous |
 |
500 ml |
50.00 |
Add to Cart |
RT |
26116-02 |
Silver Nitrate, 1% Aqueous |
 |
250 ml |
53.00 |
Add to Cart |
0-4°C |
26116-03 |
Gum Mastic, 2.5% |
|
250 ml |
53.00 |
Add to Cart |
0-4°C |
26116-04 |
Hydroquinone, 2% |
 |
250 ml |
38.00 |
Add to Cart |
RT |
26116-05 |
Silver Nitrate, 0.04% |
|
250 ml |
24.00 |
Add to Cart |
RT |
26116-06 |
Alcian Blue, pH 2.5 |
 |
250 ml |
49.00 |
Add to Cart |
RT |
26116-07 |
Harris Hematoxylin |
 |
500 ml |
30.00 |
Add to Cart |
RT |
26116-08 |
Acid Alcohol, 1% |
|
500 ml |
26.00 |
Add to Cart |
RT |
26116-09 |
Ammonia Water, 0.25% |
 |
500 ml |
13.00 |
Add to Cart |
RT |
26116-10 |
Eosin Y, 1% Aqueous |
 |
500 ml |
33.00 |
Add to Cart |

Modified Steiner – Using Chapman's Modification
For bacterial stain.
Stain results:
Spirochetes, Donovan Bodies, General
Bacteria,Legionairies Disease Bacteria: |
|
Dark Brown or Black |
Background: |
|
Bright Yellow to light brown |
References:
- Margeson L.S. Chapman CM: Use of Zinc Formalin as a sensitizer in Silver Stain for pirochetes. Journal of Histology 19:2, p 135-138, 6-96 (1996)
RT |
26119-01 |
Zinc Formalin |
 |
500 ml |
26.00 |
Add to Cart |
RT |
26119-02 |
Silver Nitrate, 1% Aqueous |
 |
250 ml |
48.00 |
Add to Cart |
0-4°C |
26119-03 |
Gum Mastic, 2.5% |
 |
500 ml |
66.00 |
Add to Cart |
0-4°C |
26119-04 |
Hydroquinone, 2% |
 |
500 ml |
54.00 |
Add to Cart |
RT |
26119-05 |
Silver Nitrate, 0.04%, Aqueous |
 |
250 ml |
19.00 |
Add to Cart |
A.2 FUNGAL STAINS

Gridley's Method for Fungi
For stain fungal mycelia, conidia, morphological detail of yeast forms and hyphae.
Stain results:
Mycelia, Conidia: |
|
Deep Purple |
Elastic Tissue & Mucin Background: |
|
Yellow |
References:
- Gridley, M.F.: American J. Clin. Path., 23:303 (1953)
- AFIP Manual of Histologic Staining Techniques: 3rd., Ed. ED. L. Luna, New York: McGraw-Hill Publications, c. 1968, p. 229
RT |
26120-01 |
Chromic Acid, 4%, Aqueous |
 |
500 ml |
26.00 |
Add to Cart |
0-4°C |
26120-02 |
Coleman's Feulgen Reagent |
 |
250 ml |
25.00 |
Add to Cart |
RT |
26120-03 |
Aldehyde Fuchsin Solution |
 |
250 ml |
26.00 |
Add to Cart |
RT |
26120-04 |
Metanil Yellow, 0.25% |
 |
500 ml |
27.00 |
Add to Cart |

A Combined Hematoxylin and Eosin/Methenamine Silver Stain for the Histological Diagnosis of Fungi in Tissue Sections
For bacterial, fungal stains.
Stain results:
Organism: |
|
Blue-Black |
Background: |
|
Rose |
References:
- Lamps, Laura W. et al A Combined Hematoxylin and Eosin/Methenamin Silver Stain for theHistological Diagnosis of Fungi In Tissue Sections., J. of Histotechnology 23:4 p.341 – 345, 2000.
RT |
26123-01 |
Chromic Acid, 10% |
 |
500 ml |
42.00 |
Add to Cart |
RT |
26123-02 |
Sodium Bisulfite, 1% |
 |
500 ml |
19.00 |
Add to Cart |
RT |
26123-03 |
Silver Nitrate, 5% Aqueous |
 |
250 ml |
89.00 |
Add to Cart |
RT |
26123-04 |
Methenamine, 3% Aqueous |
 |
500 ml |
25.00 |
Add to Cart |
RT |
26123-05 |
Sodium Borate, 5% Aqueous |
 |
500 ml |
29.00 |
Add to Cart |
RT |
26123-06 |
Gold Chloride, 0.2% Aqueous |
 |
250 ml |
62.00 |
Add to Cart |
RT |
26123-07 |
Sodium Thiosulfate, 0.2% Aqueous |
 |
500 ml |
19.00 |
Add to Cart |
RT |
26123-08 |
Harris' Hematoxylin |
 |
500 ml |
30.00 |
Add to Cart |
RT |
26123-09 |
Acetic Acid 3% |
 |
500 ml |
16.00 |
Add to Cart |
RT |
26123-10 |
Ammonia Water, 0.25% |
 |
500 ml |
17.00 |
Add to Cart |
RT |
26123-11 |
Eosin Y, 1% Alcoholic |
 |
500 ml |
31.00 |
Add to Cart |
A.3 INCLUSION BODY STAINS

Pinkerton's Method for Rickettsia
For inclusion body stain.
Stain results:
Rickettsia: |
|
Bright Red |
Nuclei: |
|
Blue |
References:
- Simmons, J.S. and Gentzkow, C.J., Laboratory Methods of the United States Army 5th Ed., Lee & Febiger, Philadelphia, p. 572, 1944
- Luna, L.G., (ed) Manual of Histologic Staining Methods or the AFIP, 3rd Ed., McGraw-Hill, N.Y., . 237, c 1968
RT |
26131-01 |
Methylene Blue Solution, 1% Aqueous Solution |
 |
500 ml |
30.00 |
Add to Cart |
RT |
26131-02 |
Basic Fuchsin, 0.25%, Aqueous |
 |
500 ml |
25.00 |
Add to Cart |
RT |
26131-03 |
Citric Acid, 0.5% Aqueous Solution |
 |
500 ml |
15.00 |
Add to Cart |
RT |
26131-04 |
Gram's Iodine Solution OR |
 |
500 ml |
23.00 |
Add to Cart |
RT |
26131-4A |
Lugol's Iodine |
 |
500 ml |
24.00 |
Add to Cart |
RT |
26131-05 |
Sodium Thiosulfate, 5% Aqueous Solution |
 |
500 ml |
20.00 |
Add to Cart |

Wolbach's Giemsa Method
Wolbach, Todd, and Palfrey (1922)
Nuclei, collagen.
Stain results:
Nuclei, Bacteria: |
|
Blue |
Rickettsia: |
|
Purple |
Collagen, other tissue elements: |
|
Pink to rose |
References:
- Wolbach, S.B., Todd, J.L. and Palfrey, F.W., The Etilogy of Pathology Typhus, Harvard University Press, Cambridge, MA., p. 13 – 14,c 1922
- Luna, L.G., (ed), Manual of Histologic Staining Methods of the AFIP, 3rd Edition, McGraw-Hill, Y, p. 119, c. 1968
RT |
26132-01 |
Giemsa Stock Solution 250 ml OR |
|
250 ml |
33.00 |
Add to Cart |
RT |
26132-1A |
Giemsa Working Solution |
 |
500 ml |
33.00 |
Add to Cart |
RT |
26132-02 |
Rosin Alcohol Stock Solution, 10%
OR |
|
500 ml |
32.00 |
Add to Cart |
RT |
26132-2A |
Rosin Alcohol Working Solution |
|
500 ml |
28.00 |
Add to Cart |
RT |
26132-03 |
Lugol's Iodine
OR |
 |
500 ml |
24.00 |
Add to Cart |
RT |
26132-3A |
Gram's Iodine |
|
500 ml |
23.00 |
Add to Cart |
RT |
26132-04 |
Sodium Thiosulfate, 5% Aqueous |
|
500 ml |
20.00 |
Add to Cart |