Counting Chambers
63509-01
63509-02
EMS Sedgewick-Rafter Counting Cells
Intended for use with standard or inverted compound microscopes at low magnifications.
Intended for use with standard or inverted compound microscopes at low magnifications. Cat. #63509-02 features a millimeter square grid, providing the ability to extrapolate the number of zooplankton, thus saving time by not having to count the entire cell.
Both cells include two cover glasses and one plastic case.
- Slide is 1" x 3"
- Cell size is 1.96" W x 0.787" H x 0.04" D
- Cell volume is 1.0 ml
Note: Standard Methods recommended way to count zooplankton in the 400 to 1000µ range.
| 63509-01 | EMS Sedgewick-Rafter Counting Cell, Brass, 1.0 ml | each | 48.00 | Add to Cart |
| 63509-02 | EMS Gridded Sedwick-Rafter Counting Cell | each | 136.00 | Add to Cart |
Counting Chambers from Hausser
These chambers are the finest quality, optically ground, and polished milled glass chambers available. The chamber is diamond etched and has a double improved Neubauer Ruling, which has a worldwide reputation in hospitals and laboratories for unmatched reliability, meeting the most demanding of standards. The standard Hausser blood counting chambers are one piece construction (measuring 75 mm x 32 mm x 4.5 mm) ensuring long term durability and absolute accuracy in measurement and count.
We offer both the standard (incline)
and the new "V-Load" counting hambers for different charging methods. The tolerances are (2% of the volume). Cell Depth: 0.100 mm (±2%); Volume: 0.1 Microliter Ruling; Pattern: Improved Neubauer, 1/400 Square mm. Rulings cover 9 square millimeters. Boundary lines of the Neubauer rulings are the center lines of the groups of three. (These are indicated in the illustration ). The central square millimeter is ruled into 25 groups of 16 small squares, each group separated by triple lines, the middle one of which is the boundary. The ruled surface is 0.10 mm below the cover glass, so that the volume over each of 16.
Made to Last
In addition to the standard ruling patterns which are etched permanently onto the polished surface, we offer the popular "Bright-Line" counting chamber. This bright-line counting chamber allows the ruling to appear brightly illuminated providing sharp contrast to the bright lines and cells, helping the user with accurate counts and less eyestrain. The triple lines show clearly which cells lie within the counting area and eliminate guesswork.We guarantee that all marks are permanent, and can not be removed even after repeated washings.
Other Chambers and Accessories
In addition to the blood counting chambers, we also offer a variety of other counting chambers and accessories which compliment our discipline.
- The Phase Hemacytometer: used for phase microscopy; it is a one piece brightlined hemacytometer with a reduced thickness.
- The Petroff Hausser Counting Chamber: used for bacteria counting.
- The Sperm Counting Chambers: used primarily in fertility testing; it is offered in a series of depths (10, 20, 40 microns).
- The Howard Mold Counter and the Sedgewick Rafter Counting Chamber are also available as well as a series of hemacytometer and counting chamber cover glasses.
Double Neubauer Ruled Metallized Counting Chamber
An "H" shape moat which forms 2 counting areas are metal plated and double Neubauer ruled providing sharp bright line contrast for consistent cell counts. A "V" cut on each area is used to control the possibility of overflow. Triple dividing lines clearly show cells within the counting area. The ruled surface is 0.1 mm below the cover glass; liquid capacity is 0.1 mm3/mm2 and 0.00025 mm3/grid square.
Ordering: The complete set includes one counting chamber, two 0.5 mm cover glasses, one red cell pipette, one white blood cell pipette, tubing, mouthpieces and carrying case.
| Catalog # | Description | Hauser No. | Price | |
| 63510-10 | Complete Chamber Set | 3120 | 420.00 | Add to Cart |
| 63510-11 | Chamber with 2 Cover Glasses | 3100 | 322.00 | Add to Cart |
| 63510-13 | Chamber only | 3110 | 298.00 | Add to Cart |
| 63510-20 | Phase Counting Chamber | 3200 | 354.00 | Add to Cart |
Etched – Transparent Field with Opaque Pattern Levy Double Neubauer
Ruled Hemacytometer
Double improved Neubauer rulings are diamond etched directly into a polished recess to reduce damage from scratches. Opaque surfaces above and below the counting area offer an easy focal reference. The chamber comes with a quick orientation mark for the microscope. 0.1 mm cell depth.
Ordering: The complete set includes one counting chamber, two 0.5 mm cover glasses, pipettes, and carrying case.
| Catalog # | Description | Hauser No. | Price | |
| 63511-10 | Complete Set | 3520 | 480.00 | Add to Cart |
| 63511-11 | Chamber with Two Cover Glasses | 3500 | 322.00 | Add to Cart |
| 63511-13 | Chamber only | 3510 | 322.00 | Add to Cart |
Other Counting Chambers
Fuchs-Rosenthal Counting Chamber
Designed for counting leukocytes, eosinophils and cells in cerebrospinal fluid. Grid pattern is etched directly onto the glass surface. The chamber is 0.2 mm, deep.
Ordering: Chamber set includes: one chamber and 2 octagonal cover glasses.
| Catalog # | Description | Hauser No. | Price | |
| 63512-10 | Fuchs Resenthal Counting Chamber | 3720 | 458.00 | Add to Cart |
Petroff-Hausser Counting Chamber
New one piece construction ensures durability and accuracy, featuring an improved Neubauer ruling on a single plateau (1/400 sq. mm). Designed for counting bacteria, sperm and blood platelets is offered in a series of cell-depths (10 and 20 microns). Cell-depth is 0.02 mm deep with 1.5 mm thick glass slide allows for the use of dark field microscopy.
| Catalog # | Description | Hauser No. | Price | |
| 63512-20 | Petroff Hausser Chamber/w 2 Cover Glasses | 3900 | 675.00 | Add to Cart |
| 63512-21 | Petroff-Hausser Chamber w/ Case | 3920 | 765.00 | Add to Cart |
| 63512-22 | Petroff-Hausser Chamber no grid, w/ Case | 3900SP | 660.00 | Add to Cart |
| 63512-23 | Petroff-Hausser Chamber 10 microns w/ 2 Cover Glasses | 3901 | 875.00 | Add to Cart |
| 63512-24 | Petroff-Hausser Chamber 20 microns w/ 2 Cover Glasses | 3902 | 875.00 | Add to Cart |
| 63512-25 | Petroff-Hausser Chamber 10 microns w/o Grid | 3951 | 875.00 | Add to Cart |
| 63512-26 | Petroff-Hausser Chamber 20 microns w/o Grid | 3952 | 875.00 | Add to Cart |
Nageotte Counting Chamber
Bright Line double ruling, 0.5 mm cell depth.
| Catalog # | Description | Hauser No. | Price | |
| 63512-30 | Nageotte Chamber with Coverglasses | 4000 | 665.00 | Add to Cart |
Palmer, McMaster, Speirs-Levy and Howard Chambers
| Catalog # | Description | Hauser No. | Price | |
| 63512-50 | Palmer Chamber (Nanoplankton) | 3850 | 320.00 | Add to Cart |
| 63512-75 | McMaster Egg Slide Chamber | 3875 | 458.00 | Add to Cart |
| 63512-56 | Speirs-Levy Eosinophil Counting Chamber | 3556 | 460.00 | Add to Cart |
| 63512-55 | Howard Mold Counter with Two Cover Slips | 3820 | 435.00 | Add to Cart |
| McMaster Egg Slide Chamber |
| Speirs-Levy Eosinophil Counting Chamber |
| Howard Mold Counter |
Leica Bright-Line Counting Chamber
Designed by Leica for accurate counting of blood cells platelets, dust/yeast cluster and specimen samples. Sharp contrast from rhodium plated on "V-Load". Triple dividing lines clearly delineate counting area for accurate and precise results. Molded from a single piece of thermal and shock resistant glass with a polished chamber and cover glass assuring precise capillary loading and even cell distribution. Fluid volume is 0.1 mm3/mm2 and 0.00025 mm2 per each square.
Ordering: The complete set includes: one counting chamber, two 0.4 mm cover glass, one red cell pipette, one white cell pipette, tubing, mouthpieces, and storage case
| Catalog # | Description | Hauser | Price | |
| 63514-10 | Bright-Line Chamber Set | 1483 | 399.00 | Add to Cart |
| 63514-11 | Bright-Line Chamber w two 0.4 mm Cover Glass | 1492 | 312.00 | Add to Cart |
| 63514-12 | Bright-Line Chamber Only | 1490 | 280.00 | Add to Cart |
| 63514-20 | Bright-Line Phase Chamber Set w/o Cover Glass | 1475 | 332.00 | Add to Cart |
| 63514-30 | Hemacytometer Cover Glass, 0.4 mm | 1461 | 32.00 | Add to Cart |
| 63514-31 | Red Cell Pipette (Thoma) | 1456 | 57.00 | Add to Cart |
| 63514-32 | White Cell Pipette (Thoma) | 1458 | 57.00 | Add to Cart |
| 63514-34 | Plastic Case for Hemacytometer | 1505 | 56.00 | Add to Cart |
Accessories
| 63516-10 | Cover Glass 20 x 26 x 0.5 mm | 5000 | 18.00 | Add to Cart |
| 63516-11 | Cover Glass 25 x 28 x 0.5 mm | 5010 | 55.00 | Add to Cart |
| 63516-12 | Cover Glass 25 x 33 x 0.5 mm | 5020 | 55.00 | Add to Cart |
| 63516-13 | Cover Glass 27 x 37 x 0.5 mm | 5030 | 55.00 | Add to Cart |
| 63516-20 | Sedgewick Rafter Cover Glass | 5040 | 47.00 | Add to Cart |
| 63516-30 | Petroff-Hausser Reinforced Cover Glass | 5050 | 149.00 | Add to Cart |
| 63516-31 | Petroff-Hausser Cover Glass 20 x 26 x 0.2 mm | 5051 | 55.00 | Add to Cart |
| 63516-40 | Whipple Eyepiece Micrometer | 5060 | 428.00 | Add to Cart |
| 63516-50 | Nageotte Cover Glass, 30 x 33 x 0.5 mm | 5400 | 55.00 | Add to Cart |
| 63516-61 | Nageotte Cover Glass 30 x 33 x 1.5 mm | 5411 | 64.00 | Add to Cart |
| 63516-45 | Howard Thin Cover Glass 28 x 33 x 0.5 mm Thick | 5080 | 55.00 | Add to Cart |
| 63516-46 | Howard Thick Cover Glass 28 x 33 x 1 mm Thick | 5090 | 55.00 | Add to Cart |
C-Chip Disposable Hemacytometer
The world's first truly disposable Hemacytometer, the C-Chip offers precise volume control and is easy to use.
Features
- Quartz grade optical plastic
- Precise design leads to highly reproducible results
- Two counting chambers fitted with clearest grid pattern rich in contrast
- Exposure to infectious material is reduced due to its closed system
- Continuous sample containment
- Light and unbreakable compared to glass
- No need for cover slips
- Time and cost saving
- For single use
Applications
- Blood Analysis Blood Cell Counting
- Cell Culture Cell Concentration measurement / Cell Viability Test
- Microbiology Bacteria and Fungal Spore Counting
- IVF, IUI Sperm Counting
Specifications
| Measurements | 25(W) x 75(L) x 1.6(T) mm |
| Chamber Volume | 10 micro liters |
| Chamber Depth | 100, 200 or 10 Micron (Depends on model) |
Ordering Information
| 63508-01 | Neubauer Improved, 100 tests | 2 tests/slide | 50 slides | 135.00 | Add to Cart |
| 63508-03 | Fuchs Rosenthal | 2 tests/slide | 50 slides | 135.00 | Add to Cart |
| 63508-06 | Burker | 2 tests/slide | 50 slides | 135.00 | Add to Cart |
| 63508-07 | Burker Turk | 2 tests/slide | 50 slides | 135.00 | Add to Cart |
| 63508-10 | Malassez | 2 tests/slide | 50 slides | 135.00 | Add to Cart |
Cellometer™ – The Disposable Cell Counting Chamber
Cell Counting Made Easy
These Disposable Cell Counters are an enclosed chamber with two ports for sample introduction. The chamber contains precisely spaced lines in a grid pattern. There are two counting chambers per slide for replicates. The counting grid pattern is the Improved Neubauer, as in a common Hemacytometer. The consistent pattern design allows the standard cell counting procedure to be followed.
With a pipetor, 15 to 20 microlilter cell-containing sample is introduced into the counting chamber through one of the ports. Liquid spreads inside the chamber, replaces air, which escapes through the opposing port.
The cell counting chamber is placed under a phase contrast optical microscope with a typical magnification of 100. The microscope is focused both onto the grid pattern and the cell particles. Standard protocols are followed for cell counting.
- Count the total number of cells found in 4 large corner squares (C_total)
- Cell concentration = Dilution x C_total / Volume
Let the Cellometer™ replace your Hemacytometers, which are traditionally used for cell counting
Benefits of the Cellometer™
- No washing after counting. Extremely important for people whom work with infectious samples.
- No need to place the cover slide
- Easy sample introduction. By integrating the cover slide into the counting chamber, the Cellometer™ eliminates the mistakes that may be caused by misplacement of the cover slide or misleading of the sample – two major sources for counting errors when using the Hemacytometer.
- Made from plastic. Ideal for BioSafety level 3.
Technical Data
Comparisons of Counting Protocols
| Step | Cellometer™ | Hemacytometer |
| 1 | Mix cell suspension | Mix cell suspension |
| 2 | Pipette 15 to 20 microliter into sample port | Carefully load with 10 microliter solution |
| 3 | Count cells in 4 corner squares (C_total) | Count cells in 4 corner squares (C_total) |
| 4 | Cell Concentration = Dilution x (C_total / 4.0 ) x10 4 | Cell Concentration = Dilution x (C_total / 4.0 ) x10 4 |
| 5 | Dispose used Cellometer | Wash and dry cover slide and base |
Description of the experiment
- Cell Type: Human T lymphocyte
- Counting Devices: Cellometer™ with glass base (CG2), all plastic Cellometer™ (CP2) and a standard bright line Hemacytometer.
- Cell counting performed alternating between Cellometer™ and Hemacytometer.
- Statistical data analysis using Two-Sample T test.
Results
| CP2 Test 1 | CP2 Test 2 | CG2 | |
| # of test (Cellometer™) | 15 | 19 | 17 |
| # of test (Hemacytometer) | 8 | 17 | 17 |
| Mean (Cellometer™) | 1.78 x 105 | 1.83 x 105 | 1.87 x 105 |
| Mean (Hemacytometer) | 1.85 x 105 | 1.82 x 105 | 1.82 x 105 |
| p-value | 0.325 | 0.908 | 0.505 |
Conclusions
- Cell counts obtained using Cellometer™ and Hemacytometer are consistent.
- With Cellometer™, sample loading is simple and consistent.
- There is no washing and drying after using Cellometer™, which eliminates liquid waste and Saves time and effort
Ordering Information
| 63560-25 | Cellometer™ Disposable Cell Counting Chamber Glass Base | 25/bx | 140.00 | Add to Cart |
| 63561-25 | Cellometer™ Disposable Cell Counting Chamber Plastic Base | 25/bx | 140.00 | Add to Cart |
| 63561-100 | Cellometer™ Disposable Cell Counting Chamber Plastic Base | 100/bx | 425.00 | Add to Cart |
EMS Specialty Counting Chambers
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This range of quality counting chambers are available in a wide range of types for Haemocytometry, Parasitology, Cytology and other applications.
Chambers with inside markings have rulings applied using engraving techniques to give a permanent image, clearly visible through the glass without sacrificing light transmission.
Chambers with ruled cover glass have robust image applied using chrome deposition.
Volume Cell
These are used to create precise volumes of liquid to enable counting of particles in a specified volume of liquid. A thick slide (glass or plastic) has an area cut into its surface that will hold precise volumes of bathing fluid. The shallow well, facilitating easy counting of contained particles. In use, a single drop of liquid is placed on the slide and a coverglass is placed on the platform providing equal and accurate subdivisions of the fluid each of which can be observed for particle counting.
1. Sedgewick Rafter and Cover Glass - S50, S51, S52
For use in water analysis, culture inspection, and any fluid where particles per unit volume need to be determined. Available in two versions:
S50 is an economically priced plastic cell.
S52 is made from glass, with a chromium surface image. This is intended for professional use and when using phase contrast. In each type, a central cell is filled with fluid and a coverglass limits the volume to 1 milliliter. The grid subdivides this into micro-liters.
S51 is the spare cover glass for S50 and S52. Each cell is supplied with one cover glass.
| Pattern | Catalog # | Description | Price | ||
| S50 | 68050-50 | S50, Sedgewick Rafter, Plastic | each | 51.00 | Add to Cart |
| S52 | 68050-52 | S52, Sedgewick Rafter, Glass | each | 250.00 | Add to Cart |
| S51 | 68050-51 | S51, Cover Glass for S50 & S52 | each | 15.00 | Add to Cart |
2. Howard
The Howard cell is a glass slide 76 mm x 35 mm with a central circular island and is used for counting mold fibers and spores in fruit juices, especially from tomatoes. With the K20 coverglass in place a 0.1 mm thickness of liquid is contained over the central island. The coverglass has 25 calibrated fields of 1.382 mm, diameter through which to view the particles. This coverglass removes the necessity of precise adjustment of the microscope and eyepiece graticule in the original Howard method.
| Pattern | Catalog # | Description | Price | ||
| S60 | 68051-60 | Howard Cell for Fruit Juices | each | 340.00 | Add to Cart |
| K20 | 68051-20 | K20 Calibrated Cover Glass | each | 100.00 | Add to Cart |
3. Makler
For Rapid Semen Analysis – The Makler counting chamber is only 10 microns deep, which is one tenth the depth of other hemacytometers, making it the shallowest of known chambers. This quality chamber is constructed from two pieces of optically flat glass: the first is the chamber, the second is the cover glass, which has a fine grid pattern of 1 mm squares and a center area further subdivided into 0.1 mm squares. Four quartz pins of precise height hold the cover glass to give an exact trapped specimen depth of 10 microns.
| Catalog | Description | Price | ||
| 68052-01 | Makler Counting Chamber | each | 1,695.00 | Add to Cart |
4. Haemocytometry
| Pattern | Description | Application | Qty | Catalog Number* | Price | |
| Malassez | Double Cell 0.2 mm, Standard French Rule | Hemoglobin and Leucocyte Counts | each |  68052-10 |
105.00 | Add to Cart |
| Malassez | Single Cell 0.2 mm, Standard French Rule | Hemoglobin and Leucocyte Counts | each |  68052-11 |
105.00 | Add to Cart |
| Thoma | Double Cell 0.1 mm, Standard German Rule | Hemoglobin and Leucocyte Counts | each |  68052-12 |
105.00 | Add to Cart |
| Thoma | Single Cell 0.1 mm, Standard German Rule | Hemoglobin and Leucocyte Counts | each |  68052-13 |
105.00 | Add to Cart |
| Neubauer | Double Cell 0.1 mm, Modified Thoma Rule | Hemoglobin and Leucocyte Counts | each |  68052-14 |
105.00 | Add to Cart |
| Neubauer | Single Cell 0.1 mm, Modified Thoma Rule | Hemoglobin and Leucocyte Counts | each |  68052-15 |
105.00 | Add to Cart |
| Improved Neubauer | Double Cell 0.1 mm, Ruling allows full use central squares | Hemoglobin and Leucocyte Counts | each |  68052-16 |
105.00 | Add to Cart |
| Burker | Double Cell 0.1 mm, For counts in bands | Hemoglobin and Leucocyte Counts | each |  68052-17 |
105.00 | Add to Cart |
| Agasse-LeFont R | Single Cell 0.001 mm, Completely Decomal | Hemoglobin and Leucocyte Counts | each |  68052-18 |
140.00 | Add to Cart |
* All complete with case and 1 pair of cover glasses (except the Howard Cell which doesn't include a cover glass and the Sedgewick Rafter which includes one cover glass).
5. Parasitology, Cytology & Others
| Pattern | Description | Application | Qty | Catalog Number* | Price | |
| Nagoette | Double Cell 0.5 mm, For Liquids in Poor content | Urinary, Cytology, Cephalo-rachidien fluid analysis | each |  68052-20 |
250.00 | Add to Cart |
| Nagoette | Single Cell 0.5 mm, For Liquids in Poor content | Urinary, Cytology, Cephalo-rachidien fluid analysis | each |  68052-21 |
110.00 | Add to Cart |
| Lemaur | Single Cell 0.4 mm, Improved Nateotte with smaller volume | Urinary, Cytology, Cephalo-rachidien fluid analysis | each |  68052-22 |
119.00 | Add to Cart |
| McMaster | Double Cell 1.5 mm; Worm Egg counting | Bacteria | each |  68052-23 |
360.00 | Add to Cart |
| Helber | Single Round Cell; 0.02 mm; Slide 1 mm thick with Thoma Ruling | Bacteria | each |  68052-24 |
200.00 | Add to Cart |
| Evaluation | Single Round Cell; 0.02 mm; Slide 1 mm thick without Thoma Ruling | Bacteria | each | 68052-30 | 440.00 | Add to Cart |
| Agasse-LeFont B | Single Cell 0.003 mm; Completely Decimal | Other | each |  68052-26 |
142.00 | Add to Cart |
* All complete with case and 1 pair of cover glasses (except the Howard Cell which doesn't include a cover glass and the Sedgewick Rafter which includes one cover glass).
6. Chamber Cover Glasses
| Pattern | Description | Application | Qty | Catalog Number* | Price | |
| McMaster, Old | Cover Glass | Parasitology | each |  68052-27 |
80.00 | Add to Cart |
| Cover Glass | 24x24 | Suitable for all counting chambers | 10/pk |  68052-28 |
60.00 | Add to Cart |
| Cover Glass | 30 x 28 mm | Suitable for larger counting chambers | 10/pk |  68052-29 |
75.00 | Add to Cart |
