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Buffers
All of our ready-to-use buffers are:
- Conveniently packaged.
- Prepared fresh for each order.
- Economical and time-saving.
- Desired pH should be specified when ordering.
The buffer is a vehicle for the fixative and requires certain properties:
- To maintain constant pH during the time of the fixation. The pH of the fixative in EM is adjusted using the buffer system chosen; e.g. range 6.5-8.0.
- The ionic constitution to prevent the extraction of cellular components, or the deposition of precipitates of fixative chemicals.
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Product Name
Ordering Info
Acetate Buffer pH 3.6-5.6
Acetic Acid and Sodium Acetate, 0.2M, pH range 3.6-5.6. Ideal for maintaining a constant pH use during fixation, our buffers are prepared fresh for each order and conveniently packaged.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:2-8°C | Cat #: 11480 | Description: Acetate Buffer, 0.2M, pH 3.6-5.6 | MSDS | Pack: 225 mL | Price: $24.36 |
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| Storage:2-8°C | Cat #: 11482 | Description: Acetate Buffer, 0.2M, pH 3.6-5.6 | MSDS | Pack: 500 mL | Price: $29.44 |
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| Storage:2-8°C | Cat #: 11482-1L | Description: Acetate Buffer, 0.2M, pH 3.6-5.6 | MSDS | Pack: 1 L | Price: $57.86 |
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Acetate Buffer
Ideal for maintaining a constant pH use during fixation, our buffers are prepared fresh for each order and conveniently packaged.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:2-8°C | Cat #: 11482-42 | Description: Acetate Buffer, 0.2M pH 4.2 | MSDS | Pack: 500 mL | Price: $29.44 |
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| Storage:2-8°C | Cat #: 11482-42-1L | Description: Acetate Buffer, 0.2M, pH 4.2 | MSDS | Pack: 1 L | Price: $57.86 |
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| Storage:2-8°C | Cat #: 11482-50 | Description: Acetate Buffer, 0.2M pH 5.0 | MSDS | Pack: 500 mL | Price: $29.44 |
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| Storage:2-8°C | Cat #: 11482-50-1L | Description: Acetate Buffer, 0.2M, pH 5.0 | MSDS | Pack: 1 L | Price: $57.86 |
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| Storage:2-8°C | Cat #: 11482-56 | Description: Acetate Buffer, 0.2M pH 5.6 | MSDS | Pack: 500 mL | Price: $29.44 |
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| Storage:2-8°C | Cat #: 11482-56-1L | Description: Acetate Buffer, 0.2M, pH 5.6 | MSDS | Pack: 1 L | Price: $57.86 |
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Antibody Diluent
Buffer for diluting your primary and secondary antibodies, even if stored in refrigerator. Minimizes nonspecific binding of the antibodies, negative effects of disturbing substances and low or medium affinity cross-reactivities of the antibodies.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:4-8°C | Cat #: 62713 | Description: Antibody Diluent | MSDS | Pack: 50 mL | Price: $211.12 |
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| Storage:4-8°C | Cat #: 62714 | Description: Antibody Diluent | MSDS | Pack: 125 mL | Price: $400.92 |
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| Storage:4-8°C | Cat #: 62715 | Description: Antibody Diluent | MSDS | Pack: 500 mL | Price: $1,395.62 |
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| Storage:4-8°C | Cat #: 62713-01 | Description: Antibody Diluent for Frozen Sections | MSDS | Pack: 50 mL | Price: $2,019.85 |
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| Storage:4-8°C | Cat #: 62714-01 | Description: Antibody Diluent for Frozen Sections | MSDS | Pack: 125 mL | Price: $265.93 |
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Antibody Dilution Buffer, with Immunoglobulin- Free BSA
Ready-to-use for the dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB . Contains immunoglobulin free BSA and does not contain phosphate, sodium azide, or mercury preservatives.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:2-8°C | Cat #: 25886-01 | Description: Antibody Dilution Buffer, Free Bsa | MSDS | Pack: 100 mL | Price: $90.00 |
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| Storage:2-8°C | Cat #: 25886-05 | Description: Antibody Dilution Buffer, Free Bsa | MSDS | Pack: 500 mL | Price: $242.32 |
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| Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Cat #: 1227SK | Description: Antigen Preserve Solution Vii | MSDS | Pack: 1 L | Price: $187.78 |
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Borate Buffer
Ideal for use with fixation, our buffers are prepared fresh for each order and conveniently packaged.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:RT | Cat #: 11455-78 | Description: Borate Buffer, pH 7.8 | MSDS | Pack: 500 mL | Price: $42.63 |
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| Storage:RT | Cat #: 11455-82 | Description: Borate Buffer, pH 8.2 | MSDS | Pack: 500 mL | Price: $44.66 |
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| Storage:RT | Cat #: 11455-90 | Description: Borate Buffer, pH 9.0 | MSDS | Pack: 500 mL | Price: $44.66 |
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Citrate Buffer
Citric acid and Sodium citrate 0.1M with a pH range of 4.6-6.2. Ideal for use with fixation, our buffers are prepared fresh for each order and conveniently packaged.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:2-8°C | Cat #: 11490 | Description: Citrate Buffer, 0.1M, pH 4.6-6.2 | MSDS | Pack: 225 mL | Price: $24.36 |
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| Storage:2-8°C | Cat #: 11492 | Description: Citrate Buffer, 0.1M, pH 4.6-6.2 | MSDS | Pack: 500 mL | Price: $29.44 |
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| Storage:2-8°C | Cat #: 11492-1L | Description: Citrate Buffer, 0.1M, pH 4.6-6.2 | Pack: 1 L | Price: $51.76 |
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| Storage:2-8°C | Cat #: 11492-04 | Description: Citrate Buffer 0.2M, pH 4.0 | MSDS | Pack: 1 L | Price: $53.80 |
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| Storage:2-8°C | Cat #: 11492-06 | Description: Citrate Buffer 0.2M, pH 6.0 | MSDS | Pack: 1 L | Price: $53.80 |
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Citrate Buffer pH 6.0 (10x)
Buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. Using this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:2-8°C | Cat #: 64142-07 | Description: Citrate Buffer pH 6.0 | MSDS | Pack: 100 mL | Price: $85.50 |
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| Storage:2-8°C | Cat #: 64142-08 | Description: Citrate Buffer pH 6.0 | MSDS | Pack: 1000 mL | Price: $145.00 |
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| Storage:2-8°C | Cat #: 64142-08-1G | Description: Citrate Buffer pH 6.0 | MSDS | Pack: 1 gal | Price: $391.79 |
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Dulbecco's Phosphate Buffer Saline – D-PBS
All solutions contain: Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Prepared 1X strength.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:RT | Cat #: 11772-05 | Description: D-PBS, Formula I, 0.15M | MSDS | Pack: 500 mL | Price: $35.52 |
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| Storage:RT | Cat #: 11772-10 | Description: D-PBS, Formula I, 0.15M | MSDS | Pack: 1 L | Price: $40.60 |
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| Storage:RT | Cat #: 11772-10-1G | Description: D-PBS, Formula I, 0.15M | MSDS | Pack: 1 gal | Price: $112.00 |
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| Storage:RT | Cat #: 11773-05 | Description: D-PBS, Formula II, 0.19M | MSDS | Pack: 500 mL | Price: $34.51 |
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| Storage:RT | Cat #: 11773-10 | Description: D-PBS, Formula II, 0.19M | MSDS | Pack: 1 L | Price: $42.63 |
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| Storage:RT | Cat #: 11773-10-1G | Description: D-PBS, Formula II, 0.19M | Pack: 1 gal | Price: $96.42 |
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| Storage:RT | Cat #: 11774-05 | Description: D-PBS, Formula III, 0.15M | MSDS | Pack: 500 mL | Price: $40.60 |
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| Storage:RT | Cat #: 11774-10 | Description: D-PBS, Formula III, 0.15M | MSDS | Pack: 1 L | Price: $45.68 |
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| Storage:RT | Cat #: 11774-10-1G | Description: D-PBS, Formula III, 0.15M | Pack: 1 gal | Price: $95.92 |
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EARLE's Balanced Salt Solutions - EBSS
All Earle's Balanced Salt Solution contains: Potassium Chloride, Sodium Chloride, Sodium Phosphate Monobasic, and D-glucose. Some formulations may contain additional components. All of the solutions are prepared 1X strength.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:RT | Cat #: 11765-05 | Description: EBSS, Formula I, 0.157M | MSDS | Pack: 500 mL | Price: $29.44 |
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| Storage:RT | Cat #: 11765-10 | Description: EBSS, Formula I, 0.157M | MSDS | Pack: 1 L | Price: $34.51 |
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| Storage:RT | Cat #: 11765-10-1G | Description: EBSS, Formula I, 0.157M | Pack: 1 gal | Price: $100.99 |
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| Storage:RT | Cat #: 11766-05 | Description: EBSS, Formula II, 0.152M | MSDS | Pack: 500 mL | Price: $29.44 |
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| Storage:RT | Cat #: 11766-10 | Description: EBSS, Formula II, 0.152M | MSDS | Pack: 1 L | Price: $36.54 |
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| Storage:RT | Cat #: 11766-10-1G | Description: EBSS, Formula II, 0.152M | Pack: 1 gal | Price: $100.99 |
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| Storage:RT | Cat #: 11767-05 | Description: EBSS, Formula III, 0.157M | MSDS | Pack: 500 mL | Price: $29.44 |
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| Storage:RT | Cat #: 11767-10 | Description: EBSS, Formula III, 0.157M | MSDS | Pack: 1 L | Price: $36.54 |
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| Storage:RT | Cat #: 11767-10-1G | Description: EBSS, Formula III, 0.157M | Pack: 1 gal | Price: $100.99 |
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EDTA Buffer pH 8.5 (10x)
Buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. Using this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:2-8°C | Cat #: 64142-09 | Description: EDTA Buffer pH 8.5 (10x) | MSDS | Pack: 100 mL | Price: $111.00 |
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| Storage:2-8°C | Cat #: 64142-10 | Description: EDTA Buffer pH 8.5 (10x) | MSDS | Pack: 1000 mL | Price: $161.34 |
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| Storage:2-8°C | Cat #: 62142-10-1G | Description: EDTA Buffer pH 8.5 (10x) | MSDS | Pack: 1 gal | Price: $355.25 |
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Hepes 0.2M Buffer Solution
Ideal for maintaining a constant pH use during fixation, our buffers are prepared fresh for each order and conveniently packaged. Specify pH when ordering.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:RT | Cat #: 11494 | Description: Hepes 0.2M Buffer Solution | MSDS | Pack: 500 mL | Price: $28.42 |
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HRP Conjugate Diluent
Specifically designed for preparing solution of your HRP-conjugate used as the detection reagent. It is the Antibody-dilutent buffer with additional component for stabilizing your HRP-conjugate. Allows you to further standardize the assay.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:4-8°C | Cat #: 62716 | Description: HRP Conjugate Diluent | MSDS | Pack: 50 mL | Price: $200.97 |
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| Storage:4-8°C | Cat #: 62717 | Description: HRP Conjugate Diluent | MSDS | Pack: 125 mL | Price: $457.76 |
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| Storage:4-8°C | Cat #: 62718-15 | Description: Slide Washing Buffer | MSDS | Pack: 500 mL | Price: $133.98 |
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Michel's Buffer
Ideal for maintaining a constant pH use during fixation, our buffers are prepared fresh for each order and conveniently packaged. Specify pH when ordering.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:RT | Cat #: 18041-05 | Description: Michel's Buffer | MSDS | Pack: 500 mL | Price: $28.42 |
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| Storage:RT | Cat #: 18041-10 | Description: Michel's Buffer | MSDS | Pack: 1 L | Price: $37.56 |
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| Storage:RT | Cat #: 18041-10-1G | Description: Michel's Buffer | MSDS | Pack: 1 gal | Price: $116.72 |
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Millonig's Phosphate Buffer
Use this buffer for the fixation of very hydrated tissues with osmium tetroxide. Osmolarity is 440 mosmols, so buffer is hypertonic to most body fluids.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:2-8°C | Cat #: 11582-05 | Description: Millonig’s Phosphate Buffer 0.2M | MSDS | Pack: 500 mL | Price: $29.94 |
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| Storage:2-8°C | Cat #: 11582-10 | Description: Millonig’s Phosphate Buffer 0.2M | MSDS | Pack: 1 L | Price: $40.60 |
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| Storage:2-8°C | Cat #: 11582-10-1G | Description: Millonig’s Phosphate Buffer 0.2M | MSDS | Pack: 1 gal | Price: $146.16 |
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| Storage:2-8°C | Cat #: 11582-10-5G | Description: Millonig’s Phosphate Buffer 0.2M | MSDS | Pack: 5 gal | Price: $654.68 |
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| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:RT | Cat #: 18900 | Description: Mops, Biological Buffer | MSDS | Pack: 100 g | Price: $87.80 |
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PHEM Buffer
PHEM buffer has been used primarily for tissues and cell cultures being processed for immunocytochemical studies. Most antigens, especially intra cellular ones, stain better using PHEM than those processed using PBS.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:2-8°C | Cat #: 11162 | Description: PHEM Buffer, 0.1M | MSDS | Pack: 225 mL | Price: $62.93 |
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| Storage:2-8°C | Cat #: 11163 | Description: PHEM Buffer, 0.1M | MSDS | Pack: 500 mL | Price: $107.59 |
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| Storage:2-8°C | Cat #: 11164 | Description: PHEM Buffer, 0.2M | MSDS | Pack: 225 mL | Price: $62.93 |
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| Storage:2-8°C | Cat #: 11165 | Description: PHEM Buffer, 0.2M | MSDS | Pack: 500 mL | Price: $120.16 |
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Phosphate Buffer, 0.1M*
Prepared fresh for each order from Sodium Phosphate Monobasic Hydrate and Sodium Phosphate Dibasic Heptahydrate. Non-Sterile. Meant for biological research only.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:2-8°C | Cat #: 19340-60 | Description: Phosphate Buffer pH 6.0 | MSDS | Pack: 1 L | Price: $48.47 |
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| Storage:2-8°C | Cat #: 19340-60-1G | Description: Phosphate Buffer, pH 6.0 | MSDS | Pack: 1 gal | Price: $126.88 |
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| Storage:2-8°C | Cat #: 19340-65 | Description: Phosphate Buffer pH 6.5 | MSDS | Pack: 1 L | Price: $48.47 |
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| Storage:2-8°C | Cat #: 19340-65-1G | Description: Phosphate Buffer, pH 6.5 | MSDS | Pack: 1 gal | Price: $126.88 |
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| Storage:2-8°C | Cat #: 19340-70 | Description: Phosphate Buffer pH 7.0 | MSDS | Pack: 1 L | Price: $48.47 |
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| Storage:2-8°C | Cat #: 19340-70-1G | Description: Phosphate Buffer, pH 7.0 | MSDS | Pack: 1 gal | Price: $126.88 |
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| Storage:2-8°C | Cat #: 19340-72 | Description: Phosphate Buffer pH 7.2 | MSDS | Pack: 1 L | Price: $48.47 |
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| Storage:2-8°C | Cat #: 19340-72-1G | Description: Phosphate Buffer, pH 7.2 | MSDS | Pack: 1 gal | Price: $126.88 |
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Phosphate Buffered Saline (PBS)
Commonly used in biochemistry, it is a salty solution containing sodium chloride, potassium chloride, sodium phosphate and potassium phosphate. Isotonic and non-toxic to cells. 1X PBS final concentration is 8.0 g/L NaCl, 0.2 g/L KCl, 1.42 g/L Na2HPO4, 0.24 g/L KH2PO4 and pH 7.4.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:2-8°C | Cat #: 19342-10 | Description: Phosphate Buffered Saline, 10X | MSDS | Pack: 1 L | Price: $53.25 |
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| Storage:2-8°C | Cat #: 19242-40 | Description: Phosphate Buffered Saline, 10X | MSDS | Pack: 1 gal | Price: $216.00 |
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| Storage:2-8°C | Cat #: 19343-10 | Description: Phosphate Buffered Saline, 2X | MSDS | Pack: 1 L | Price: $34.51 |
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| Storage:2-8°C | Cat #: 19343-40 | Description: Phosphate Buffered Saline, 2X | MSDS | Pack: 1 gal | Price: $106.58 |
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| Storage:2-8°C | Cat #: 19344-10 | Description: Phosphate Buffered Saline, 1X | MSDS | Pack: 1 L | Price: $28.67 |
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| Storage:2-8°C | Cat #: 19344-20 | Description: Phosphate Buffered Saline, 1X | MSDS | Pack: 1 gal | Price: $92.36 |
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Pipes Buffer Solution, 0.3M
PIPES is a hydrogen-ion organic-based buffer, used in the preparation of a variety of mammalian tissues for EM. PIPES solution is adjusted by 0.1N Sodium Hydroxide to pH 5.5 - 6.0.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:RT | Cat #: 11610 | Description: Pipes Buffer Solution, 0.3M | MSDS | Pack: 500 mL | Price: $63.44 |
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