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Buffers

All of our ready-to-use buffers are:

  • Conveniently packaged.
  • Prepared fresh for each order.
  • Economical and time-saving.
  • Desired pH should be specified when ordering.

The buffer is a vehicle for the fixative and requires certain properties:

  1. To maintain constant pH during the time of the fixation. The pH of the fixative in EM is adjusted using the buffer system chosen; e.g. range 6.5-8.0.
  2. The ionic constitution to prevent the extraction of cellular components, or the deposition of precipitates of fixative chemicals.
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Picture of Acetate Buffer pH 3.6-5.6

Acetate Buffer pH 3.6-5.6

Acetic Acid and Sodium Acetate, 0.2M, pH range 3.6-5.6. Ideal for maintaining a constant pH use during fixation, our buffers are prepared fresh for each order and conveniently packaged.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:2-8°C Cat #: 11480 Description: ACETATE BUFFER, 0.2M, pH 3.6-5.6 MSDS Pack: 225 mL Price: $21.00 Add to Quote:
Storage:2-8°C Cat #: 11482 Description: ACETATE BUFFER, 0.2M, pH 3.6-5.6 MSDS Pack: 500 mL Price: $26.00 Add to Quote:
Storage:2-8°C Cat #: 11482-1L Description: Acetic Acid and Sodium Acetate 0.2M MSDS Pack: 1 L Price: $51.00 Add to Quote:
Picture of Acetate Buffer

Acetate Buffer

Ideal for maintaining a constant pH use during fixation, our buffers are prepared fresh for each order and conveniently packaged.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:2-8°C Cat #: 11482-42 Description: Acetate Buffer, 0.2m pH 4.2 MSDS Pack: 500 mL Price: $26.00 Add to Quote:
Storage:2-8°C Cat #: 11482-42-1L Description: Acetate Buffer, 0.2M, pH 4.2 MSDS Pack: 1 L Price: $51.00 Add to Quote:
Storage:2-8°C Cat #: 11482-50 Description: Acetate Buffer, 0.2m pH 5.0 MSDS Pack: 500 mL Price: $26.00 Add to Quote:
Storage:2-8°C Cat #: 11482-50-1L Description: Acetate Buffer, 0.2M, pH 5.0 MSDS Pack: 1 L Price: $51.00 Add to Quote:
Storage:2-8°C Cat #: 11482-56 Description: Acetate Buffer, 0.2m ph 5.6 MSDS Pack: 500 mL Price: $26.00 Add to Quote:
Storage:2-8°C Cat #: 11482-56-1L Description: Acetate Buffer, 0.2M, pH 5.6 MSDS Pack: 1 L Price: $51.00 Add to Quote:
Picture of Antibody Diluent

Antibody Diluent

Buffer for diluting your primary and secondary antibodies, even if stored in refrigerator. Minimizes nonspecific binding of the antibodies, negative effects of disturbing substances and low or medium affinity cross-reactivities of the antibodies.
Technical Data Sheet Technical Data Sheet
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:4-8°C Cat #: 62713 Description: Antibody Diluent MSDS Pack: 50 mL Price: $189.00 Add to Quote:
Storage:4-8°C Cat #: 62714 Description: Antibody Diluent MSDS Pack: 125 mL Price: $413.00 Add to Quote:
Storage:4-8°C Cat #: 62715 Description: Antibody Diluent MSDS Pack: 500 mL Price: $1,540.00 Add to Quote:
Storage:4-8°C Cat #: 62713-01 Description: Antibody Diluent for Frozen Sections MSDS Pack: 50 mL Price: $189.00 Add to Quote:
Storage:4-8°C Cat #: 62714-01 Description: Antibody Diluent for Frozen Sections MSDS Pack: 125 mL Price: $413.00 Add to Quote:
Storage:4-8°C Cat #: 62715-01 Description: Antibody Diluent for Frozen Sections MSDS Pack: 500 mL Price: $1,540.00 Add to Quote:
Picture of Antibody Dilution Buffer, with Immunoglobulin- Free BSA

Antibody Dilution Buffer, with Immunoglobulin- Free BSA

Ready-to-use for the dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB . Contains immunoglobulin free BSA and does not contain phosphate, sodium azide, or mercury preservatives.
Technical Data Sheet
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:2-8°C Cat #: 25886-01 Description: Antibody Dilution Buffer, free BSA MSDS Pack: 100 mL Price: $73.00 Add to Quote:
Storage:2-8°C Cat #: 25886-05 Description: Antibody Dilution Buffer, free BSA MSDS Pack: 500 mL Price: $196.00 Add to Quote:
Picture of Antigen Preserve Solution VII

Antigen Preserve Solution VII

Antigen Preserve Solution VII is available for your convenience.
Technical Data Sheet
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:  Cat #: 1227SK Description: Antigen Preserve Solution VII MSDS Pack: 1 L Price: $172.00 Add to Quote:
Picture of Borate Buffer

Borate Buffer

Ideal for use with fixation, our buffers are prepared fresh for each order and conveniently packaged.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:RT Cat #: 11455-78 Description: Borate Buffer, pH 7.8 MSDS Pack: 500 mL Price: $37.00 Add to Quote:
Storage:RT Cat #: 11455-82 Description: Borate Buffer, pH 8.2 MSDS Pack: 500 mL Price: $39.00 Add to Quote:
Storage:RT Cat #: 11455-90 Description: Borate Buffer, pH 9.0 MSDS Pack: 500 mL Price: $39.00 Add to Quote:
Picture of Citrate Buffer

Citrate Buffer

Citric acid and Sodium citrate 0.1M with a pH range of 4.6-6.2. Ideal for use with fixation, our buffers are prepared fresh for each order and conveniently packaged.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:2-8°C Cat #: 11490 Description: CITRATE BUFFER, 0.1M, pH 4.6-6.2 MSDS Pack: 225 mL Price: $21.00 Add to Quote:
Storage:2-8°C Cat #: 11492 Description: CITRATE BUFFER, 0.1M, pH 4.6-6.2 MSDS Pack: 500 mL Price: $26.00 Add to Quote:
Storage:2-8°C Cat #: 11492-1L Description: Citrate Buffer, 0.1M Pack: 1 L Price: $46.00 Add to Quote:
Storage:2-8°C Cat #: 11492-04 Description: Citrate Buffer 0.2M, pH 4.0 MSDS Pack: 1 L Price: $47.00 Add to Quote:
Storage:2-8°C Cat #: 11492-06 Description: Citrate Buffer 0.2M, pH 6.0 MSDS Pack: 1 L Price: $47.00 Add to Quote:
Picture of Citrate Buffer pH 6.0 (10x)

Citrate Buffer pH 6.0 (10x)

Buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. Using this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Technical Data Sheet
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:2-8°C Cat #: 64142-07 Description: Citrate Buffer pH 6.0 MSDS Pack: 100 mL Price: $86.00 Add to Quote:
Storage:2-8°C Cat #: 64142-08 Description: Citrate Buffer pH 6.0 MSDS Pack: 1000 mL Price: $143.00 Add to Quote:
Storage:2-8°C Cat #: 64142-08-1G Description: Citrate Buffer pH 6.0 MSDS Pack: 1 gal Price: $358.00 Add to Quote:
Picture of Dulbecco's Phosphate Buffer Saline – D-PBS

Dulbecco's Phosphate Buffer Saline – D-PBS

All solutions contain: Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Prepared 1X strength.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:RT Cat #: 11772-05 Description: D-PBS, Formula I MSDS Pack: 500 mL Price: $29.00 Add to Quote:
Storage:RT Cat #: 11772-10 Description: D-PBS, Formula I MSDS Pack: 1 L Price: $34.00 Add to Quote:
Storage:RT Cat #: 11772-10-1G Description: D-PBS, Formula I, 0.15M MSDS Pack: 1 gal Price: $75.00 Add to Quote:
Storage:RT Cat #: 11773-05 Description: D-PBS, Formula II MSDS Pack: 500 mL Price: $29.00 Add to Quote:
Storage:RT Cat #: 11773-10 Description: D-PBS, Formula II MSDS Pack: 1 L Price: $35.00 Add to Quote:
Storage:RT Cat #: 11773-10-1G Description: D-PBS, Formula II, 0.19M Pack: 1 gal Price: $84.00 Add to Quote:
Storage:RT Cat #: 11774-05 Description: D-PBS, Formula III MSDS Pack: 500 mL Price: $34.00 Add to Quote:
Storage:RT Cat #: 11774-10 Description: D-PBS, Formula III MSDS Pack: 1 L Price: $39.00 Add to Quote:
Storage:RT Cat #: 11774-10-1G Description: D-PBS, Formula III, 0.15M Pack: 1 gal Price: $86.00 Add to Quote:
Picture of EARLE's Balanced Salt Solutions - EBSS

EARLE's Balanced Salt Solutions - EBSS

All Earle's Balanced Salt Solution contains: Potassium Chloride, Sodium Chloride, Sodium Phosphate Monobasic, and D-glucose. Some formulations may contain additional components. All of the solutions are prepared 1X strength.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:RT Cat #: 11765-05 Description: EARL'S BALANCED SALTS, FORMULA I MSDS Pack: 500 mL Price: $25.00 Add to Quote:
Storage:RT Cat #: 11765-10 Description: EARL'S BALANCED SALTS, FORMULA I MSDS Pack: 1 L Price: $30.00 Add to Quote:
Storage:RT Cat #: 11765-10-1G Description: ESBSS, Formula I, 0.157M Pack: 1 gal Price: $90.00 Add to Quote:
Storage:RT Cat #: 11766-05 Description: EARL'S BALANCED SALTS, FORMULA II MSDS Pack: 500 mL Price: $24.00 Add to Quote:
Storage:RT Cat #: 11766-10 Description: EARL'S BALANCED SALTS, FORMULA II MSDS Pack: 1 L Price: $30.00 Add to Quote:
Storage:RT Cat #: 11766-10-1G Description: ESBSS, Formula II, 0.152M Pack: 1 gal Price: $90.00 Add to Quote:
Storage:RT Cat #: 11767-05 Description: EARL'S BALANCED SALTS, FORMULA III MSDS Pack: 500 mL Price: $24.00 Add to Quote:
Storage:RT Cat #: 11767-10 Description: EARL'S BALANCED SALTS, FORMULA III MSDS Pack: 1 L Price: $30.00 Add to Quote:
Storage:RT Cat #: 11767-10-1G Description: ESBSS, Formula III, 0.157M Pack: 1 gal Price: $90.00 Add to Quote:
Picture of EDTA Buffer pH 8.5 (10x)

EDTA Buffer pH 8.5 (10x)

Buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. Using this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Technical Data Sheet
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:2-8°C Cat #: 64142-09 Description: EDTA Buffer pH 8.5 MSDS Pack: 100 mL Price: $86.00 Add to Quote:
Storage:2-8°C Cat #: 64142-10 Description: EDTA Buffer pH 8.5 MSDS Pack: 1000 mL Price: $143.00 Add to Quote:
Storage:2-8°C Cat #: 62142-10-1G Description: EDTA Buffer pH 8.5 MSDS Pack: 1 gal Price: $330.00 Add to Quote:
Picture of Hepes Buffer Solution

Hepes Buffer Solution

Ideal for maintaining a constant pH use during fixation, our buffers are prepared fresh for each order and conveniently packaged. Shecify pH when ordering.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:RT Cat #: 11494 Description: HEPES BUFFER SOLUTION,0.2M,pH ? MSDS Pack: 500 mL Price: $25.00 Add to Quote:
Picture of HRP Conjugate Diluent

HRP Conjugate Diluent

Specifically designed for preparing solution of your HRP-conjugate used as the detection reagent. It is the Antibody-dilutent buffer with additional component for stabilizing your HRP-conjugate. Allows you to further standardize the assay.
Technical Data Sheet
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:4-8°C Cat #: 62716 Description: HRP Conjugate Diluent MSDS Pack: 50 mL Price: $189.00 Add to Quote:
Storage:4-8°C Cat #: 62717 Description: HRP Conjugate Diluent MSDS Pack: 125 mL Price: $435.00 Add to Quote:
Storage:4-8°C Cat #: 62718 Description: HRP Conjugate Diluent MSDS Pack: 500 mL Price: $1,551.00 Add to Quote:
Storage: Cat #: 62718-15 Description: Slide Washing Buffer MSDS Pack: 500 mL Price: $149.00 Add to Quote:
Picture of Michel's Buffer

Michel's Buffer

Ideal for maintaining a constant pH use during fixation, our buffers are prepared fresh for each order and conveniently packaged. Shecify pH when ordering.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:RT Cat #: 18041-05 Description: Michel's Buffer MSDS Pack: 500 mL Price: $25.00 Add to Quote:
Storage:RT Cat #: 18041-10 Description: Michel's Buffer MSDS Pack: 1 L Price: $33.00 Add to Quote:
Storage:RT Cat #: 18041-10-1G Description: Michel’s Buffer MSDS Pack: 1 gal Price: $105.00 Add to Quote:
Storage:RT Cat #: 18041-10-5G Description: Michel’s Buffer MSDS Pack: 5 gal Price: $495.00 Add to Quote:
Picture of Millonig's Phosphate Buffer

Millonig's Phosphate Buffer

Use this buffer for the fixation of very hydrated tissues with osmium tetroxide. Osmolarity is 440 mosmols, so buffer is hypertonic to most body fluids.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:2-8°C Cat #: 11582-05 Description: MILLONIG'S PHOSPHATE BUFFER, .2M MSDS Pack: 500 mL Price: $26.00 Add to Quote:
Storage:2-8°C Cat #: 11582-10 Description: MILLONIG'S PHOSPHATE BUFFER, .2M MSDS Pack: 1 L Price: $36.00 Add to Quote:
Storage:2-8°C Cat #: 11582-10-1G Description: Millonig’s Phosphate Buffer 0.2M MSDS Pack: 1 gal Price: $132.00 Add to Quote:
Storage:2-8°C Cat #: 11582-10-5G Description: Millonig’s Phosphate Buffer 0.2M MSDS Pack: 5 gal Price: $578.00 Add to Quote:
Picture of Mops, Biological Buffer

Mops, Biological Buffer

(3-(N-Morpholino) propanesulfonic acid) pH range 6.5-7.9
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:RT Cat #: 18900 Description: MOPS, Biological Buffer MSDS Pack: 100 g Price: $81.00 Add to Quote:
Picture of PHEM Buffer

PHEM Buffer

PHEM buffer has been used primarily for tissues and cell cultures being processed for immunocytochemical studies. Most antigens, especially intra cellular ones, stain better using PHEM than those processed using PBS.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:2-8°C Cat #: 11162 Description: PHEM Buffer MSDS Pack: 225 mL Price: $50.00 Add to Quote:
Storage:2-8°C Cat #: 11163 Description: PHEM Buffer MSDS Pack: 500 mL Price: $84.00 Add to Quote:
Storage:2-8°C Cat #: 11164 Description: PHEM Buffer, 0.2M MSDS Pack: 225 mL Price: $55.00 Add to Quote:
Storage:2-8°C Cat #: 11165 Description: PHEM Buffer, 0.2M MSDS Pack: 500 mL Price: $92.00 Add to Quote:
Picture of Phosphate Buffer, 0.1M*

Phosphate Buffer, 0.1M*

Prepared fresh for each order from Sodium Phosphate Monobasic Hydrate and Sodium Phosphate Dibasic Heptahydrate. Non-Sterile. Meant for biological research only.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:2-8°C Cat #: 19340-60 Description: Phosphate Buffer pH 6.0 MSDS Pack: 1 L Price: $44.00 Add to Quote:
Storage:2-8°C Cat #: 19340-60-1G Description: Phosphate Buffer, pH 6.0 MSDS Pack: 1 gal Price: $114.00 Add to Quote:
Storage:2-8°C Cat #: 19340-65 Description: Phosphate Buffer pH 6.5 MSDS Pack: 1 L Price: $44.00 Add to Quote:
Storage:2-8°C Cat #: 19340-65-1G Description: Phosphate Buffer, pH 6.5 MSDS Pack: 1 gal Price: $114.00 Add to Quote:
Storage:2-8°C Cat #: 19340-70 Description: Phosphate Buffer pH 7.0 MSDS Pack: 1 L Price: $44.00 Add to Quote:
Storage:2-8°C Cat #: 19340-70-1G Description: Phosphate Buffer, pH 7.0 MSDS Pack: 1 gal Price: $114.00 Add to Quote:
Storage:2-8°C Cat #: 19340-72 Description: Phosphate Buffer pH 7.2 MSDS Pack: 1 L Price: $44.00 Add to Quote:
Storage:2-8°C Cat #: 19340-72-1G Description: Phosphate Buffer, pH 7.2 MSDS Pack: 1 gal Price: $114.00 Add to Quote:
Picture of Phosphate Buffer Saline (PBS)

Phosphate Buffer Saline (PBS)

Commonly used in biochemistry, it is a salty solution containing sodium chloride, potassium chloride, sodium phosphate and potassium phosphate. Isotonic and non-toxic to cells. 1X final conc is 0.137M NaCl, 0.01M Na2HPO4, 0.0027M KCl and pH 7.4.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:2-8°C Cat #: 19342-10 Description: Phosphate Buffered Saline, 10X MSDS Pack: 1 L Price: $35.00 Add to Quote:
Storage:2-8°C Cat #: 19242-40 Description: Phosphate Buffered Saline, 10X MSDS Pack: 1 gal Price: $109.00 Add to Quote:
Storage:2-8°C Cat #: 19343-10 Description: Phosphate Buffered Saline, 2X MSDS Pack: 1 L Price: $31.00 Add to Quote:
Storage:2-8°C Cat #: 19343-40 Description: Phosphate Buffered Saline, 2X MSDS Pack: 1 gal Price: $97.00 Add to Quote:
Storage:2-8°C Cat #: 19344-10 Description: Phosphate Buffered Saline, 1X MSDS Pack: 1 L Price: $26.00 Add to Quote:
Storage:2-8°C Cat #: 19344-20 Description: Phosphate Buffered Saline, 1X MSDS Pack: 1 gal Price: $84.00 Add to Quote:
Picture of Pipes Buffer Solution, 0.3M

Pipes Buffer Solution, 0.3M

PIPES is a hydrogen-ion organic-based buffer, used in the preparation of a variety of mammalian tissues for EM. PIPES solution is adjusted by 0.1N Sodium Hydroxide to pH 5.5-6.0.
Technical Data Sheet
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:RT Cat #: 11610 Description: PIPES BUFFER SOLUTION, 0.3M,pH ? MSDS Pack: 500 mL Price: $56.00 Add to Quote:

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