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Buffers

All of our ready-to-use buffers are:

  • Conveniently packaged.
  • Prepared fresh for each order.
  • Economical and time-saving.
  • Desired pH should be specified when ordering.

The buffer is a vehicle for the fixative and requires certain properties:

  1. To maintain constant pH during the time of the fixation. The pH of the fixative in EM is adjusted using the buffer system chosen; e.g. range 6.5-8.0.
  2. The ionic constitution to prevent the extraction of cellular components, or the deposition of precipitates of fixative chemicals.
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Product Name Ordering Info

Acetate Buffer pH 3.6-5.6

Acetic Acid and Sodium Acetate, 0.2M, pH range 3.6-5.6. Ideal for maintaining a constant pH use during fixation, our buffers are prepared fresh for each order and conveniently packaged.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:2-8°C Cat #: 11480 Description: ACETATE BUFFER, 0.2M, pH 3.6-5.6 Pack: 225 mL Price: $21.00 Add to Quote:
Storage:2-8°C Cat #: 11482 Description: ACETATE BUFFER, 0.2M, pH 3.6-5.6 Pack: 500 mL Price: $26.00 Add to Quote:
Storage:2-8°C Cat #: 11482-1L Description: Acetic Acid and Sodium Acetate 0.2M Pack: 1 L Price: $51.00 Add to Quote:

Acetate Buffer

Ideal for maintaining a constant pH use during fixation, our buffers are prepared fresh for each order and conveniently packaged.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:2-8°C Cat #: 11482-42 Description: Acetate Buffer, 0.2m pH 4.2 Pack: 500 mL Price: $26.00 Add to Quote:
Storage:2-8°C Cat #: 11482-42-1L Description: Acetate Buffer, 0.2M, pH 4.2 Pack: 1 L Price: $51.00 Add to Quote:
Storage:2-8°C Cat #: 11482-50 Description: Acetate Buffer, 0.2m pH 5.0 Pack: 500 mL Price: $26.00 Add to Quote:
Storage:2-8°C Cat #: 11482-50-1L Description: Acetate Buffer, 0.2M, pH 5.0 Pack: 1 L Price: $51.00 Add to Quote:
Storage:2-8°C Cat #: 11482-56 Description: Acetate Buffer, 0.2m ph 5.6 Pack: 500 mL Price: $26.00 Add to Quote:
Storage:2-8°C Cat #: 11482-56-1L Description: Acetate Buffer, 0.2M, pH 5.6 Pack: 1 L Price: $51.00 Add to Quote:

Antibody Diluent

Buffer for diluting your primary and secondary antibodies, even if stored in refrigerator. Minimizes nonspecific binding of the antibodies, negative effects of disturbing substances and low or medium affinity cross-reactivities of the antibodies.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:4-8°C Cat #: 62713 Description: Antibody Diluent Pack: 50 mL Price: $189.00 Add to Quote:
Storage:4-8°C Cat #: 62714 Description: Antibody Diluent Pack: 125 mL Price: $413.00 Add to Quote:
Storage:4-8°C Cat #: 62715 Description: Antibody Diluent Pack: 500 mL Price: $1,540.00 Add to Quote:
Storage:4-8°C Cat #: 62713-01 Description: Antibody Diluent for Frozen Sections Pack: 50 mL Price: $189.00 Add to Quote:
Storage:4-8°C Cat #: 62714-01 Description: Antibody Diluent for Frozen Sections Pack: 125 mL Price: $413.00 Add to Quote:
Storage:4-8°C Cat #: 62715-01 Description: Antibody Diluent for Frozen Sections Pack: 500 mL Price: $1,540.00 Add to Quote:

Antibody Dilution Buffer, with Immunoglobulin- Free BSA

Ready-to-use for the dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB . Contains immunoglobulin free BSA and does not contain phosphate, sodium azide, or mercury preservatives.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:2-8°C Cat #: 25886-01 Description: Antibody Dilution Buffer, free BSA Pack: 100 mL Price: $73.00 Add to Quote:
Storage:2-8°C Cat #: 25886-05 Description: Antibody Dilution Buffer, free BSA Pack: 500 mL Price: $196.00 Add to Quote:

Antigen Preserve Solution VII

Antigen Preserve Solution VII is available for your convenience.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:  Cat #: 1227SK Description: Antigen Preserve Solution VII Pack: 1 L Price: $172.00 Add to Quote:

Borate Buffer

Ideal for use with fixation, our buffers are prepared fresh for each order and conveniently packaged.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:RT Cat #: 11455-78 Description: Borate Buffer, pH 7.8 Pack: 500 mL Price: $37.00 Add to Quote:
Storage:RT Cat #: 11455-82 Description: Borate Buffer, pH 8.2 Pack: 500 mL Price: $39.00 Add to Quote:
Storage:RT Cat #: 11455-90 Description: Borate Buffer, pH 9.0 Pack: 500 mL Price: $39.00 Add to Quote:

Citrate Buffer

Citric acid and Sodium citrate 0.1M with a pH range of 4.6-6.2. Ideal for use with fixation, our buffers are prepared fresh for each order and conveniently packaged.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:2-8°C Cat #: 11490 Description: CITRATE BUFFER, 0.1M, pH 4.6-6.2 Pack: 225 mL Price: $21.00 Add to Quote:
Storage:2-8°C Cat #: 11492 Description: CITRATE BUFFER, 0.1M, pH 4.6-6.2 Pack: 500 mL Price: $26.00 Add to Quote:
Storage:2-8°C Cat #: 11492-1L Description: Citrate Buffer, 0.1M Pack: 1 L Price: $46.00 Add to Quote:
Storage:2-8°C Cat #: 11492-04 Description: Citrate Buffer 0.2M, pH 4.0 Pack: 1 L Price: $47.00 Add to Quote:
Storage:2-8°C Cat #: 11492-06 Description: Citrate Buffer 0.2M, pH 6.0 Pack: 1 L Price: $47.00 Add to Quote:

Citrate Buffer pH 6.0 (10x)

Buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. Using this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:2-8°C Cat #: 64142-07 Description: Citrate Buffer pH 6.0 Pack: 100 mL Price: $86.00 Add to Quote:
Storage:2-8°C Cat #: 64142-08 Description: Citrate Buffer pH 6.0 Pack: 1000 mL Price: $143.00 Add to Quote:
Storage:2-8°C Cat #: 64142-08-1G Description: Citrate Buffer pH 6.0 Pack: 1 gal Price: $358.00 Add to Quote:

Dulbecco's Phosphate Buffer Saline – D-PBS

All solutions contain: Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Prepared 1X strength.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:RT Cat #: 11772-05 Description: D-PBS, Formula I Pack: 500 mL Price: $29.00 Add to Quote:
Storage:RT Cat #: 11772-10 Description: D-PBS, Formula I Pack: 1 L Price: $34.00 Add to Quote:
Storage:RT Cat #: 11772-10-1G Description: D-PBS, Formula I, 0.15M Pack: 1 gal Price: $75.00 Add to Quote:
Storage:RT Cat #: 11773-05 Description: D-PBS, Formula II Pack: 500 mL Price: $29.00 Add to Quote:
Storage:RT Cat #: 11773-10 Description: D-PBS, Formula II Pack: 1 L Price: $35.00 Add to Quote:
Storage:RT Cat #: 11773-10-1G Description: D-PBS, Formula II, 0.19M Pack: 1 gal Price: $84.00 Add to Quote:
Storage:RT Cat #: 11774-05 Description: D-PBS, Formula III Pack: 500 mL Price: $34.00 Add to Quote:
Storage:RT Cat #: 11774-10 Description: D-PBS, Formula III Pack: 1 L Price: $39.00 Add to Quote:
Storage:RT Cat #: 11774-10-1G Description: D-PBS, Formula III, 0.15M Pack: 1 gal Price: $86.00 Add to Quote:

EARLE's Balanced Salt Solutions - EBSS

All Earle's Balanced Salt Solution contains: Potassium Chloride, Sodium Chloride, Sodium Phosphate Monobasic, and D-glucose. Some formulations may contain additional components. All of the solutions are prepared 1X strength.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:RT Cat #: 11765-05 Description: EARL'S BALANCED SALTS, FORMULA I Pack: 500 mL Price: $25.00 Add to Quote:
Storage:RT Cat #: 11765-10 Description: EARL'S BALANCED SALTS, FORMULA I Pack: 1 L Price: $30.00 Add to Quote:
Storage:RT Cat #: 11765-10-1G Description: ESBSS, Formula I, 0.157M Pack: 1 gal Price: $90.00 Add to Quote:
Storage:RT Cat #: 11766-05 Description: EARL'S BALANCED SALTS, FORMULA II Pack: 500 mL Price: $24.00 Add to Quote:
Storage:RT Cat #: 11766-10 Description: EARL'S BALANCED SALTS, FORMULA II Pack: 1 L Price: $30.00 Add to Quote:
Storage:RT Cat #: 11766-10-1G Description: ESBSS, Formula II, 0.152M Pack: 1 gal Price: $90.00 Add to Quote:
Storage:RT Cat #: 11767-05 Description: EARL'S BALANCED SALTS, FORMULA III Pack: 500 mL Price: $24.00 Add to Quote:
Storage:RT Cat #: 11767-10 Description: EARL'S BALANCED SALTS, FORMULA III Pack: 1 L Price: $30.00 Add to Quote:
Storage:RT Cat #: 11767-10-1G Description: ESBSS, Formula III, 0.157M Pack: 1 gal Price: $90.00 Add to Quote:

EDTA Buffer pH 8.5 (10x)

Buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. Using this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:2-8°C Cat #: 64142-09 Description: EDTA Buffer pH 8.5 Pack: 100 mL Price: $86.00 Add to Quote:
Storage:2-8°C Cat #: 64142-10 Description: EDTA Buffer pH 8.5 Pack: 1000 mL Price: $143.00 Add to Quote:
Storage:2-8°C Cat #: 62142-10-1G Description: EDTA Buffer pH 8.5 Pack: 1 gal Price: $330.00 Add to Quote:

Hepes Buffer Solution

Ideal for maintaining a constant pH use during fixation, our buffers are prepared fresh for each order and conveniently packaged. Shecify pH when ordering.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:RT Cat #: 11494 Description: HEPES BUFFER SOLUTION,0.2M,pH ? Pack: 500 mL Price: $25.00 Add to Quote:

HRP Conjugate Diluent

Specifically designed for preparing solution of your HRP-conjugate used as the detection reagent. It is the Antibody-dilutent buffer with additional component for stabilizing your HRP-conjugate. Allows you to further standardize the assay.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:4-8°C Cat #: 62716 Description: HRP Conjugate Diluent Pack: 50 mL Price: $189.00 Add to Quote:
Storage:4-8°C Cat #: 62717 Description: HRP Conjugate Diluent Pack: 125 mL Price: $435.00 Add to Quote:
Storage:4-8°C Cat #: 62718 Description: HRP Conjugate Diluent Pack: 500 mL Price: $1,551.00 Add to Quote:
Storage: Cat #: 62718-15 Description: Slide Washing Buffer Pack: 500 mL Price: $149.00 Add to Quote:

Michel's Buffer

Ideal for maintaining a constant pH use during fixation, our buffers are prepared fresh for each order and conveniently packaged. Shecify pH when ordering.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:RT Cat #: 18041-05 Description: Michel's Buffer Pack: 500 mL Price: $25.00 Add to Quote:
Storage:RT Cat #: 18041-10 Description: Michel's Buffer Pack: 1 L Price: $33.00 Add to Quote:
Storage:RT Cat #: 18041-10-1G Description: Michel’s Buffer Pack: 1 gal Price: $105.00 Add to Quote:
Storage:RT Cat #: 18041-10-5G Description: Michel’s Buffer Pack: 5 gal Price: $495.00 Add to Quote:

Millonig's Phosphate Buffer

Use this buffer for the fixation of very hydrated tissues with osmium tetroxide. Osmolarity is 440 mosmols, so buffer is hypertonic to most body fluids.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:2-8°C Cat #: 11582-05 Description: MILLONIG'S PHOSPHATE BUFFER, .2M Pack: 500 mL Price: $26.00 Add to Quote:
Storage:2-8°C Cat #: 11582-10 Description: MILLONIG'S PHOSPHATE BUFFER, .2M Pack: 1 L Price: $36.00 Add to Quote:
Storage:2-8°C Cat #: 11582-10-1G Description: Millonig’s Phosphate Buffer 0.2M Pack: 1 gal Price: $132.00 Add to Quote:
Storage:2-8°C Cat #: 11582-10-5G Description: Millonig’s Phosphate Buffer 0.2M Pack: 5 gal Price: $578.00 Add to Quote:

Mops, Biological Buffer

(3-(N-Morpholino) propanesulfonic acid) pH range 6.5-7.9
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:RT Cat #: 18900 Description: MOPS, Biological Buffer Pack: 100 g Price: $81.00 Add to Quote:

PHEM Buffer

PHEM buffer has been used primarily for tissues and cell cultures being processed for immunocytochemical studies. Most antigens, especially intra cellular ones, stain better using PHEM than those processed using PBS.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:2-8°C Cat #: 11162 Description: PHEM Buffer Pack: 225 mL Price: $50.00 Add to Quote:
Storage:2-8°C Cat #: 11163 Description: PHEM Buffer Pack: 500 mL Price: $84.00 Add to Quote:
Storage:2-8°C Cat #: 11164 Description: PHEM Buffer, 0.2M Pack: 225 mL Price: $55.00 Add to Quote:
Storage:2-8°C Cat #: 11165 Description: PHEM Buffer, 0.2M Pack: 500 mL Price: $92.00 Add to Quote:

Phosphate Buffer, 0.1M*

Prepared fresh for each order from Sodium Phosphate Monobasic Hydrate and Sodium Phosphate Dibasic Heptahydrate. Non-Sterile. Meant for biological research only.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:2-8°C Cat #: 19340-60 Description: Phosphate Buffer pH 6.0 Pack: 1 L Price: $44.00 Add to Quote:
Storage:2-8°C Cat #: 19340-60-1G Description: Phosphate Buffer, pH 6.0 Pack: 1 gal Price: $114.00 Add to Quote:
Storage:2-8°C Cat #: 19340-65 Description: Phosphate Buffer pH 6.5 Pack: 1 L Price: $44.00 Add to Quote:
Storage:2-8°C Cat #: 19340-65-1G Description: Phosphate Buffer, pH 6.5 Pack: 1 gal Price: $114.00 Add to Quote:
Storage:2-8°C Cat #: 19340-70 Description: Phosphate Buffer pH 7.0 Pack: 1 L Price: $44.00 Add to Quote:
Storage:2-8°C Cat #: 19340-70-1G Description: Phosphate Buffer, pH 7.0 Pack: 1 gal Price: $114.00 Add to Quote:
Storage:2-8°C Cat #: 19340-72 Description: Phosphate Buffer pH 7.2 Pack: 1 L Price: $44.00 Add to Quote:
Storage:2-8°C Cat #: 19340-72-1G Description: Phosphate Buffer, pH 7.2 Pack: 1 gal Price: $114.00 Add to Quote:

Phosphate Buffer Saline (PBS)

Commonly used in biochemistry, it is a salty solution containing sodium chloride, potassium chloride, sodium phosphate and potassium phosphate. Isotonic and non-toxic to cells. 1X final conc is 0.137M NaCl, 0.01M Na2HPO4, 0.0027M KCl and pH 7.4.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:2-8°C Cat #: 19342-10 Description: Phosphate Buffered Saline, 10X Pack: 1 L Price: $35.00 Add to Quote:
Storage:2-8°C Cat #: 19242-40 Description: Phosphate Buffered Saline, 10X Pack: 1 gal Price: $109.00 Add to Quote:
Storage:2-8°C Cat #: 19343-10 Description: Phosphate Buffered Saline, 2X Pack: 1 L Price: $31.00 Add to Quote:
Storage:2-8°C Cat #: 19343-40 Description: Phosphate Buffered Saline, 2X Pack: 1 gal Price: $97.00 Add to Quote:
Storage:2-8°C Cat #: 19344-10 Description: Phosphate Buffered Saline, 1X Pack: 1 L Price: $26.00 Add to Quote:
Storage:2-8°C Cat #: 19344-20 Description: Phosphate Buffered Saline, 1X Pack: 1 gal Price: $84.00 Add to Quote:

Pipes Buffer Solution, 0.3M

PIPES is a hydrogen-ion organic-based buffer, used in the preparation of a variety of mammalian tissues for EM. PIPES solution is adjusted by 0.1N Sodium Hydroxide to pH 5.5-6.0.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:RT Cat #: 11610 Description: PIPES BUFFER SOLUTION, 0.3M,pH ? Pack: 500 mL Price: $56.00 Add to Quote: