Premixed, prebuffered fixatives, that are pH adjusted for immediate laboratory use. They are freshly prepared upon receipt of order to ensure maximum shelf life. The fixatives are filled in 20 ml glass vials (10 ml/vial). This allows for the immediate fixation of your specimens without costly and time consuming transfer associated with ampoule contained fixatives.
*If you desire any prepared fixative that is not already listed in our catalog or you have special size and packaging requirements, please call us.
Bouin's solution can be used as a fixation and a staining fluid. Bouin's fixative is excellent for use on biopsy specimens of the gastrointestinal tract. Tissue from the endocrine system are well fixed and many antibodies react well with tissue fixed in Bouin's. Bouin's solution may be used as a routine fixative or a staining solution, but should not be used as a preservative for tissue. Tissue cannot be held in Bouin's solution for extended periods. The maximum fixation time in this solution should be less than 48 hours and should be transferred to alcohol. Packaged in leak-proof histo-containers, prefilled and ready-to-use.
Formulated with Picric Acid saturated aqueous solution 75%, Formalin (40% aqueous formaldehyde) 25% and Glacial Acetic acid 5% in de-ionized water.
Ethanol, Picric Acid, Formalin, Acetic Acid, Glacial.
This fixative is most popular when glycogen is the target. The solution is similar to Bouins Solution (alcoholic) offering quick hardening and good staining with acid dyes.
EMS is proud to introduce this ready-to-use new fixative designed to offer the latest preservation of structural integrity with good immunostaining results. This fixative is a phosphate buffered ethanol (BE-70) with the addition of glycerol and glacial acetic acid.
Reference: Journal of Histochemistry & Cytochemistry 2017, Vol 65(4) p. 251-252
Millonig's phosphate buffered formalin as modified by Carson, Martin and Lynn, by using 4% formalin in 0.1M sodium phosphate monobasic with sodium hydroxide to pH 7.2.
For some tissues, the use of formalin (contains 10 – 15% Methanol) is not suitable, we offer the alternative formaldehyde (prepared from paraformaldehyde – contains no methanol) for your convenience. Contains 4% paraformaldehyde.
CytoPrep Fixative forms a clear protective coating over specimens while fixing cells. It is ideal for cytological smears and it dries within 4 to 6 minutes. Good for safe storage and transportation of slides. It is water soluble and contains no chlorofluorocarbons. Comes in a 4 oz. spray can.
Cytology fixative spray designed for slide based cytology specimens for cellular preservation and transport.
Quickly spray a fine mist over the slide (covering the entire specimen) for cellular fixation and reduce air drying of the material.
CytoFix forms a thin, tough, clear protective coating over the specimen with a water and alcohol solution of Polyethylene glycol for cellular fixation and does not require rehydration by the laboratory prior to staining. Allows safe storing or transporting of slides.
Allow specimens to dry before sending to lab or for storage.
This is rapid fixative. Results with good nuclear detail and minimal formalin pigment. Davidson's (Hartmann's) Fixative is useful for preparing tumors, bone marrow, gynecologic specimen, fatty breast, medical biopsies. Fixation of small specimen is rapid. Exposure to the fixative should be limited to 24 hours (tissue may then be transferred to neutral buffered formalin or 70% alcohol for storage).
Based on the volume of 1125 ml solution, there are 2 parts Formalin (35-39%), 3 parts Ethanol, 1 part Glacial Acetic acid, and 3 parts tap water.
A specially formulated odor-mask which neutralizes the formalin odor without affecting subsequent tissue processing, handling, or staining. Low odor, ready-to-use 10% formalin solution. May be used as a fixative or preservative of specimens. Its shelf life is 24 months.
Low odor, prepared w/v, pH 6.9 to 7.1 at 25°C. 10% formalin in Phosphate buffer according to the A.F.I.P. Manual of Histology. This solution allows for long-term storage of tissue specimens.
Prefilled with 10% formalin Cat# 15740. Ready-to-use in pre-filled cups. Labeled in accordance to OSHA requirements, which gives you immediate information regarding handling and first aid procedures. Pre-measured in wide-mouth, leak-proof, opaque polypropylene containers which limits your exposure to formaldehyde and saves you time.
The HistoTainer™ is half filled with 10% Neutral Buffered Formalin as a fixative. These ready-to-use prefilled containers are timer saving and make your work more efficient.
An alternative fixative to Formalin and Formaldehyde.
EMS is proud to introduce an alternative to Formalin and Formaldehyde in many different applications.
Our unique kit comes premixed and measured and ready to use. The kit consists of 2 vials: Solution A & Solution B (Absolute Ethanol, Glyoxal Solution, Acetic Acid and water).
Instructions
Add solution A to solution B. Vortex the two solutions together and bring to a pH of 4.5 with the addition of NaOH 1M. The solution needs to be used immediately once mixed.
References
Sabatini DD. et al (1963) "The preservation of cellular ultrastructure and enzymatic activity by
aldehyde fixation". J. Cell Biology. 17:19-58
Janigan DT. et al (1965) "The effects of aldehyde fixation on acid phosphatase activity in tissue
blocks". J. Histochemistry. Cytochemistry. 13: 476-483
Dapson RW. et al (2006) "Glyoxal fixation and its relationship to immunocytochemistry."
J. Histotechnology. 29: 65-76
Dapson RW. Et al (2007) "Glyoxal fixation: how it works and why it only occasionally needs
antigen retrieval" lnforma healthcare, Dapson & Dapson, LLC
Richter K. et al (2018) "Glyoxal as an alternative to formaldehyde in immunostaining and
super-resolution microscopy" The EMBO Journal. 37: 139-159