Fixatives, Prepared to Fluorescein
Fixatives, Prepared
Premixed, prebuffered fixatives, that are pH adjusted for immediate laboratory use. They are freshly prepared upon receipt of order to ensure maximum shelf life. The fixatives are filled in 20 ml glass vials (10 ml/vial). This allows for the immediate fixation of your specimens without costly and time consuming transfer associated with ampoule contained fixatives.
*If you desire any prepared fixative that is not already listed in our catalog or you have special size and packaging requirements, please call us.
| 0-5°C | 15953 | 0.2% Formaldehyde/0.1% Chromic Acid in 0.1M Sodium Cacodylate Buffer, pH 7.4 | 10x10 ml | 37.00 | Add to Cart |
| 0-5°C | 15956 | 2% Formaldehyde/0.02% Picric Acid in 0.1M Sodium Phosphate Buffer, pH 7.2 | 10x10 ml | 37.00 | Add to Cart |
| 0-5°C | 15949 | Formaldehyde/Glutaraldehyde, 2.5% each in 0.1M Sodium Cacodylate Buffer, pH 7.4 | 10x10 ml | 38.00 | Add to Cart |
| 0-5°C | 15950 | Formaldehyde/Glutaraldehyde, 3% each in 0.1M Sodium Cacodylate Buffer, pH 7.4 | 10x10 ml | 38.00 | Add to Cart |
| 0-5°C | 15960 | 2.5% Glutaraldehyde in 0.1M Sodium Cacodylate Buffer, pH 7.4 | 10x10 ml | 38.00 | Add to Cart |
| 0-5°C | 15960-01 | 2% Paraformaldehyde / 2.5% Glutaraldehyde in 0.1M Sodium Cacodylate Buffer, pH 7.4 | 10x10 ml | 28.00 | Add to Cart |
| 0-5°C | 15970 | 2.5% Glutaraldehyde in 0.1M Millonig's Sodium Phosphate Buffer pH 7.2 | 10x10 ml | 37.00 | Add to Cart |
| 0-5°C | 15980 | 2.5% Glutaraldehyde in 0.1M Sorensen's Sodium-Potassium Phosphate Buffer, pH 7.2 | 10x10 ml | 37.00 | Add to Cart |
Bouin's Fixative
A fixative that is used in light microscopy for the preparation of delicate structure.
| RT | 15990 | 10x10 ml | 37.00 | Add to Cart |
B-5 Fixative
For lymph nodes.
| RT | 64122-05 | B-5 Fixative | 500 ml | 64.00 | Add to Cart |
| RT | 64122-06 | B-5 Fixative | 1 lt | 120.00 | Add to Cart |
| RT | 64122-10 | B-5 Fixative | 4 lt | 450.00 | Add to Cart |
Bouin's Solution
Bouin's solution can be used as a fixation and a staining fluid. Bouin's fixative is excellent for use on biopsy specimens of the gastrointestinal tract. Tissue from the endocrine system are well fixed and many antibodies react well with tissue fixed in Bouin's. Bouin's solution may be used as a routine fixative or a staining solution, but should not be used as a preservative for tissue. Tissue cannot be held in Bouin's solution for extended periods. The maximum fixation time in this solution should be less than 48 hours and should be transferred to alcohol. Packaged in leak-proof histo-containers, prefilled and ready-to-use.
Formulated with Picric Acid saturated aqueous solution 75%, Formalin (40% aqueous formaldehyde) 25% and Glacial Acetic acid 5% in de-ionized water.
| RT | 15990-01 | Bouin's Fluid | 1 L | 46.00 | Add to Cart |
| RT | 15990-04 | Bouin's Fluid | 4 L | 162.00 | Add to Cart |
| RT | 15990-10 | Bouin's Fluid Pre-filled cups | 50x10 ml/cs | 82.00 | Add to Cart |
| RT | 15990-20 | Bouin's Fluid Pre-filled cups | 50x20 ml/cs | 102.00 | Add to Cart |
| RT | 15990-30 | Bouin's Fluid Pre-filled cups | 20x30 ml/cs | 46.00 | Add to Cart |
Brasil Fixative
Ethanol, Picric Acid, Formalin, Acetic Acid, Glacial.
This fixative is most popular when glycogen is the target. The solution is similar to Bouins Solution (alcoholic) offering quick hardening and good staining with acid dyes.
| RT | 15995-10 | Brasil Fixative | 10x10 ml | 41.00 | Add to Cart |
| RT | 15995-20 | Brasil Fixative | 100 ml | 41.00 | Add to Cart |
Buffered Ethanol Fixative, 70% BE-70
EMS is proud to introduce this ready-to-use new fixative designed to offer the latest preservation of structural integrity with good immunostaining results. This fixative is a phosphate buffered ethanol (BE-70) with the addition of glycerol and glacial acetic acid.
Reference: Journal of Histochemistry & Cytochemistry 2017, Vol 65(4) p. 251-252
| RT | 15988 | 1 L | 70.00 | Add to Cart |
Carnoy's Fluid
Stock solution.
| RT | 64130-05 | Carnoy's Fluid | |
500 ml | 26.00 | Add to Cart |
| RT | 64130-50 | Carnoy's Fluid Without Chloroform | |
500 ml | 24.00 | Add to Cart |
Carson's Phosphate Buffered Formalin
Millonig's phosphate buffered formalin as modified by Carson, Martin and Lynn, by using 4% formalin in 0.1M sodium phosphate monobasic with sodium hydroxide to pH 7.2.
| RT | 15732-05 | Carson's Phosphate Buffered Formalin | 500 ml | 19.00 | Add to Cart |
| RT | 15732-10S | Carson's Phosphate Buffered Formalin | 1 L | 32.00 | Add to Cart |
Carson's Phosphate Buffered Paraformaldehyde
For some tissues, the use of formalin (contains 10 – 15% Methanol) is not suitable, we offer the alternative formaldehyde (prepared from paraformaldehyde – contains no methanol) for your convenience.
| RT | 15733-05 | Carson's Phosphate Buffered Paraformaldehyde | 500 ml | 22.00 | Add to Cart |
| RT | 15733-10 | Carson's Phosphate Buffered Paraformaldehyde | 1 L | 36.00 | Add to Cart |
CytoPrep™ Fixative
CytoPrep Fixative forms a clear protective coating over specimens while fixing cells. It is ideal for cytological smears and it dries within 4 to 6 minutes. Good for safe storage and transportation of slides. It is water soluble and contains no chlorofluorocarbons. Comes in a 4 oz. spray can.
| RT | 64145-01 | CytoPrep Fixative | 4 oz | 40.00 | Add to Cart |
| RT | 64145-12 | CytoPrep Fixative | 12x4 oz | 410.00 | Add to Cart |
CytoFix
Cytology fixative spray designed for slide based cytology specimens for cellular preservation and transport.
Quickly spray a fine mist over the slide (covering the entire specimen) for cellular fixation and reduce air drying of the material.
CytoFix forms a thin, tough, clear protective coating over the specimen with a water and alcohol solution of Polyethylene glycol for cellular fixation and does not require rehydration by the laboratory prior to staining. Allows safe storing or transporting of slides.
Allow specimens to dry before sending to lab or for storage.
- Dries in 4 to 6 minutes
- Water soluble
- Won't cloud or obscure specimen
- Contains no chlorofluorocarbons
Available in pump bottle or aerosol can.
| 22805-01 | CytoFix (Non-Aerosol) | 100 ml | 14.50 | Add to Cart |
| 22805-02 | CytoFix Can | 4 oz | 14.50 | Add to Cart |
Davidson's Fixative (Hartmann's/ Davidson's Fixative)
This is rapid fixative. Results with good nuclear detail and minimal formalin pigment. Davidson's (Hartmann's) Fixative is useful for preparing tumors, bone marrow, gynecologic specimen, fatty breast, medical biopsies. Fixation of small specimen is rapid. Exposure to the fixative should be limited to 24 hours (tissue may then be transferred to neutral buffered formalin or 70% alcohol for storage).
Based on the volume of 1125 ml solution, there are 2 parts Formalin (35-39%), 3 parts Ethanol, 1 part Glacial Acetic acid, and 3 parts tap water.
| RT | 64133-10 | Davidson's (Hartmann's) Fixative | 1 L | 82.00 | Add to Cart |
| RT | 64133-14 | Davidson's (Hartmann's) Fixative | 4 L | 232.00 | Add to Cart |
Modified Davidson's Fixative
(14% Ethyl Alcohol, Denatured, 37.5% Formalin 37-39%, 6.25% Glacial Acetic Acid, and D.I. Water).
| RT | 64133-50 | Modified Davidson's Fixative | 1L | 39.00 | Add to Cart |
Dietrich's Fixative
Ethanol, formalin, glacial acetic acid, distilled water.
| RT | 15958-10 | Dietrich's Fixative | 10x10 ml | 44.00 | Add to Cart |
| RT | 15958-20 | Dietrich's Fixative | 100 ml | 43.00 | Add to Cart |
Fixative/Decalcifier
- A solution that fixes and decalcifies in one step.
- Prevents loss of cellular structure.
- Enhances hematoxylin staining.
| RT | 64148-01 | Fixative/Decalcifier | 1 L | 52.00 | Add to Cart |
| RT | 64148-06 | Fixative/Decalcifier | 6x1 L | 310.00 | Add to Cart |
Formalde-Fresh Low Odor Solution; Histology Grade
A specially formulated odor-mask which neutralizes the formalin odor without affecting subsequent tissue processing, handling, or staining. Low odor, ready-to-use 10% formalin solution. May be used as a fixative or preservative of specimens. Its shelf life is 24 months.
| RT | 15678-01 | Formalde-Fresh | 4 L | 94.00 | Add to Cart |
| RT | 15678-04 | Formalde-Fresh | 4x4 L | 360.00 | Add to Cart |
Buffered Formalde-Fresh, Low-Odor 10% Formalin Solution
Low odor, prepared w/v, pH 6.9 to 7.1 at 25°C. 10% formalin in Phosphate buffer according to the A.F.I.P. Manual of Histology. This solution allows for long-term storage of tissue specimens.
| RT | 15741-01 | Low-Odor Formalin 10% Buffered | 4 L | 64.00 | Add to Cart |
| RT | 15741-04 | Low-Odor Formalin 10% Buffered | 4x4 L | 229.00 | Add to Cart |
Formalin, Neutral, Buffered 10% w/v in Acetate Buffer
Buffered with Sodium Acetate in accordance with the A.F.I.P. manual of Histology. Filtered and ready-to-use.
pH 6.0 to ph 7.1 at 20°C.
| RT | 15738-01 | Formalin 10% Buffered in Acetate | 1L | 23.00 | Add to Cart |
| RT | 15738-04 | Formalin 10% Buffered in Acetate | 4L | 55.00 | Add to Cart |
Formalin, Neutral, Buffered 10% w/v in Phosphate Buffer
Buffered with phosphate in accordance with the A.f.I.P. manual of Histology.Filtered and ready-to-use.
pH 6.9 to 7.1 at 25°C.
| RT | 15740 | Formalin 10% Buffered in Phosphate | 450 ml | 16.00 | Add to Cart |
| RT | 15740-01 | Formalin 10% Buffered in Phosphate | 1 L | 23.00 | Add to Cart |
| RT | 15740-04 | Formalin 10% Buffered in Phosphate | 4 L | 61.00 | Add to cart |
TissuePrep Buffered 10% Formalin
Prefilled with 10% formalin Cat# 15740. Ready-to-use in pre-filled cups. Labeled in accordance to OSHA requirements, which gives you immediate information regarding handling and first aid procedures. Pre-measured in wide-mouth, leak-proof, opaque polypropylene containers which limits your exposure to formaldehyde and saves you time.
| Catalog # | Cup Size | Filled | Pack/cs | Price | ||
| RT | 15742-10 | 15 ml | 10 ml | 50 | 77.00 | Add to Cart |
| RT | 15742-20 | 30 ml | 20 ml | 50 | 98.00 | Add to Cart |
| RT | 15742-30 | 60 ml | 30 ml | 20 | 44.00 | Add to Cart |
| RT | 15742-60 | 120 ml | 60 ml | 20 | 48.00 | Add to Cart |
Formalin Prefilled HistoTainer™
The HistoTainer™ (Empty Vials, see page 482) is half filled with 10% Neutral Buffered Formalin as a fixative. These ready-to-use prefilled containers are timer saving and make your work more efficient.
| EMS # | Description | Vial Size | Prefilled | Qty | |||
| RT | 15743-20 | Formalin Prefilled HistoTainer | 20 ml | 10 ml | 24 | 26.00 | Add to Cart |
| RT | 15743-40 | Formalin Prefilled HistoTainer | 40 ml | 20 ml | 24 | 30.00 | Add to Cart |
| RT | 15743-60 | Formalin Prefilled HistoTainer | 60 ml | 30 ml | 24 | 36.00 | Add to Cart |
| RT | 15743-90 | Formalin Prefilled HistoTainer | 90 ml | 45 ml | 24 | 42.00 | Add to Cart |
| RT | 15743-120 | Formalin Prefilled HistoTainer | 120 ml | 60 ml | 24 | 43.00 | Add to Cart |
Formalin Non Buffered
Formalin 1% Non Buffered
| RT | 15734-01 | Formalin 1% Non-Buffered | 1L | 21.00 | Add to Cart |
| RT | 15734-04 | Formalin 1% Non-Buffered | 4L | 56.00 | Add to Cart |
Formalin 2%, Non-Buffered
| RT | 15735-01 | Formalin 2% Non-Buffered | 1L | 21.00 | Add to Cart |
| RT | 15735-04 | Formalin 2% Non-Buffered | 4L | 56.00 | Add to Cart |
Formalin 3%, Non-Buffered
| RT | 15735-10 | Formalin 3% Non-Buffered | 1L | 22.00 | Add to Cart |
| RT | 15735-20 | Formalin 3% Non-Buffered | 4L | 57.00 | Add to Cart |
Formalin 4%, Non-Buffered
| RT | 15735-30 | Formalin 4% Non-Buffered | 1L | 22.00 | Add to Cart |
| RT | 15735-40 | Formalin 4% Non-Buffered | 4L | 56.00 | Add to Cart |
Formalin 5%, Non-Buffered
| RT | 15735-50 | Formalin 5% Non-Buffered | 1L | 23.00 | Add to Cart |
| RT | 15735-60 | Formalin 5% Non-Buffered | 4L | 57.00 | Add to Cart |
Glyoxal 3% Fixative, Ready to Use
An alternative fixative to Formalin and Formaldehyde.
EMS is proud to introduce an alternative to Formalin and Formaldehyde in many different applications.
Our unique kit comes premixed and measured and ready to use. The kit consists of 2 vials: Solution A & Solution B (Absolute Ethanol, Glyoxal Solution, Acetic Acid and water).
Instructions:
Add solution A to solution B. Vortex the two solutions together and bring to a pH of 4.5 with the addition of NaOH 1M. The solution needs to be used immediately once mixed.
References
Sabatini DD. et al (1963) "The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation". J. Cell Biology. 17:19-58
Janigan DT. et al (1965) "The effects of aldehyde fixation on acid phosphatase activity in tissue blocks". J. Histochemistry. Cytochemistry. 13: 476-483
Dapson RW. et al (2006) "Glyoxal fixation and its relationship to immunocytochemistry." J. Histotechnology. 29: 65-76
Dapson RW. Et al (2007) "Glyoxal fixation: how it works and why it only occasionally needs antigen retrieval" lnforma healthcare, Dapson & Dapson, LLC
Richter K. et al (2018) "Glyoxal as an alternative to formaldehyde in immunostaining and super-resolution microscopy" The EMBO Journal. 37: 139-159
| 2-8°C | 16525 | Glyoxal 3% Fixative | 100 ml | 49.50 | Add to Cart |
Hollande's Fixative
Please see Hollande's Fixative
Karnovsky's Fixative:
Please see Paraformaldehyde-Glutaraldehyde Solution Karnovsky's Fixative.
| RT | 15720 | kit | 27.00 | Add to Cart | |
| RT | 15730 | box of 5 kits | 120.00 | Add to Cart |
Fixative Reagent Grade (Karnovsky's)
This fixative contains 3% glutaraldehyde and 2% formaldehyde in 0.1M phosphate buffer, pH 7.4.
Karnovsky's with formalin 2% and Biological glutaraldehyde 3% in 0.1m Phosphate Buffer.
| RT | 15731-10 | Karnovsky Fixative Reagent Grade | 1 L | 258.00 | Add to Cart |
Karnovsky's with paraformaldehyde solution and EM grade glutaraldehyde
Please Note: This kit can be formulated to fit your requirements. Please contact us.
| 0-5°C | 15732-10 | Karnovsky Fixative EM Grade | 1 L | 284.00 | Add to Cart |
Paraformaldehyde, Prepared Fixatives
In addition to our complete line of Formaldehyde solutions we have now added to the line.
Paraformaldehyde 0.5% in 0.1M phosphate buffer pH7.4
| RT | 15951-10 | Paraformaldehyde, 0.5% each in 0.1M Phosphate Buffer | 10x10 ml | 38.00 | Add to Cart |
| RT | 15951-15 | Paraformaldehyde, 0.5% each in 0.1M Phosphate Buffer | 500 ml | 104.00 | Add to Cart |
Paraformaldehyde 1% Aqueous
| RT | 15953-10 | Paraformaldehyde, 1% each Aqueous | 10x10 ml | 26.00 | Add to Cart |
| RT | 15953-15 | Paraformaldehyde, 1% each Aqueous | 500 ml | 96.00 | Add to Cart |
Paraformaldehyde 1% in 0.1M Phosphate Buffer pH, 7.4
| RT | 15952-10 | Paraformaldehyde, 1% each in 0.1M Phosphate Buffer | 10x10 ml | 40.00 | Add to Cart |
| RT | 15952-15 | Paraformaldehyde, 1% each in 0.1M Phosphate Buffer | 500 ml | 104.00 | Add to Cart |
Paraformaldehyde 1% Glut 2% in 0.1M Phosphate buffer, pH 7.4
| RT | 15949-30 | Paraformaldehyde 1% Glutaraldehyde 2% in 0.1M Phosphate Buffer | 10x10 ml | 40.00 | Add to Cart |
| RT | 15949-35 | Paraformaldehyde 1% Glutaraldehyde 2% in 0.1M Phosphate Buffer | 500 ml | 107.00 | Add to Cart |
Paraformaldehyde 3% Glutaraldehyde 0.35% in 0.1M Sodium Cacodylate, pH 7.4
| RT | 15949-50 | Paraformaldehyde 3% Glutaraldehyde 0.35% in 0.1M Sodium Cacodylate Buffer | 10x10 ml | 43.00 | Add to Cart |
| RT | 15949-60 | Paraformaldehyde 3% Glutaraldehyde 0.35% in 0.1M Sodium Cacodylate Buffer | 500 ml | 114.00 | Add to Cart |
Paraformaldehyde 4% and Glutaraldehyde 1% in Phosphate Buffer, pH 7.0
| RT | 15949-70 | Paraformaldehyde 4% Glutaraldehyde 1% in 0.1M Phosphate Buffer | 10x10 ml | 47.00 | Add to Cart |
| RT | 15949-80 | Paraformaldehyde 4% Glutaraldehyde 1% in 0.1M Phosphate Buffer | 500 ml | 114.00 | Add to Cart |
Paraformaldehyde 4% in 0.1M Sodium Cacodylate, pH7.4
| RT | 15952-10S | Paraformaldehyde 4% in 0.1M Sodium Cacodylate Buffer | 10x10 ml | 50.00 | Add to Cart |
| RT | 15952-15S | Paraformaldehyde 4% in 0.1M Sodium Cacodylate Buffer | 500 ml | 130.00 | Add to Cart |
Paraformaldehyde 4% in 0.1M Phosphate Buffer, pH 7.4
| RT | 15735-10S | Paraformaldehyde 4% in 0.1M Phosphate Buffer | 10x10 ml | 38.00 | Add to Cart |
| RT | 15735-20S | Paraformaldehyde 4% in 0.1M Phosphate Buffer | 500 ml | 108.00 | Add to Cart |
Paraformaldehyde 4% in 0.2M Phosphate Buffer, pH 7.4
| RT | 15735-30S | Paraformaldehyde 4% in 0.2M Phosphate Buffer | 10x10 ml | 37.00 | Add to Cart |
| RT | 15735-40S | Paraformaldehyde 4% in 0.2M Phosphate Buffer | 500 ml | 106.00 | Add to Cart |
Paraformaldehyde 4% in 0.1M Phosphate Buffer, pH 7.2
| RT | 15735-50S | Paraformaldehyde 4% in 0.1M Phosphate Buffer | 10x10 ml | 43.00 | Add to Cart |
| RT | 15735-60S | Paraformaldehyde 4% in 0.1M Phosphate Buffer | 500 ml | 111.00 | Add to Cart |
Paraformaldehyde 4% in 0.2M Phosphate Buffer, pH 7.2
| RT | 15735-70 | Paraformaldehyde 4% in 0.2M Phosphate Buffer | 10x10 ml | 43.00 | Add to Cart |
| RT | 15735-80 | Paraformaldehyde 4% in 0.2M Phosphate Buffer | 500 ml | 109.00 | Add to Cart |
Paraformaldehyde 4% in 0.1M Phosphate BufferSaline, pH7.4
| RT | 15735-85 | Paraformaldehyde 4% in 0.1M Phosphate Buffer Saline | 10x10 ml | 44.00 | Add to Cart |
| RT | 15735-90 | Paraformaldehyde 4% in 0.1M Phosphate Buffer Saline | 500 ml | 113.00 | Add to Cart |
Paraformaldehyde 4% in 0.2M Phosphate Buffer Saline, pH 7.4
| RT | 15735-95 | Paraformaldehyde 4% in 0.2M Phosphate Buffer | 10x10 ml | 45.00 | Add to Cart |
| RT | 15735-100 | Paraformaldehyde 4% in 0.2M Phosphate Buffer | 500 ml | 114.00 | Add to Cart |
Trump's Fixative
A combination of Sodium Cacodylate, Formalin, and Glutaraldehyde. Ready to use.
| 0-5°C | 11750 | 500 ml | 45.00 | Add to Cart | |
| 0-5°C | 11752 | 4x500 ml | 179.00 | Add to Cart |
