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Electron Microscopy Sciences

Chemicals for Microscopy

Certificates of Analysis

arrow13Glycerine to HPMA

msdsarrow12Fluorescein Isothiocyanate, Isomer I, Certified (FITC)

C21H11NO5S   F.W. 389.39   CAS #3326-32-7
Assay >90%
Water soluble.

0-5°C 15670   ½g 142.00 Add to Cart

arrow12Fluoromount-G™, Slide Mountant

Please see listing in Adhesives and Mountants

msdsarrow12Formamide, Ultra High Purity

HCONH2   F.W. 45.04   CAS #75-12-7
Specific Gravity: 1.128-1.132
Glass Distilled. Exceeds A.C.S. Specifications
Specifications:

Assay   99.9%
Iron.   0.5ppm
Copper   0.1ppm
Lead   0.5ppm
Zinc   0.5ppm
RT 15745 Formamide, Ultra High Purity 100 ml 44.00 Add to Cart
RT 15746 Formamide, Ultra High Purity 500 ml 126.00 Add to Cart
RT 15747 Formamide, Ultra High Purity 4x500 ml 360.00 Add to Cart

msdsarrow12Formic Acid 96%, Reagent, A.C.S.

HCOOH   F.W. 46.03   CAS #64-18-6
Specific Gravity: 1.22

RT 15750 Formic Acid 96% 225 ml 17.00 Add to Cart
RT 15760 Formic Acid 96% 1 liter 47.00 Add to Cart

msdsarrow12Formvar 15/ 95 Resin, Powder

CAS #63450-15-7
Polyvinyl formvar desiccated. Used to prepare Support films.
Soluble in ethylene dichloride, chloroform or dioxane.

RT 15800 Formvar 15/ 95 Resin 100g 14.00 Add to Cart

arrow12Formvar Solution in Ethylene Dichloride

Microfiltered-ready to use.

RT 15810 0.25% msds 100 ml 13.00 Add to Cart
RT 15820 0.50% msds 100 ml 13.00 Add to Cart
RT 15830 1.00% msds 100 ml 14.00 Add to Cart
RT 15830-25 1.00% msds 4x25 ml 17.00 Add to Cart

*Any of the above percentages are packed in 25 ml bottles to avoid its classification as a hazardous chemical for shipping purposes.  Please specify % upon ordering.

arrow12Gach Kit

Please see listing in Embedding Media Kits.

RT 15920 Gach (Glutaraldehyde/Carbohydrazide) kit 36.00 Add to Cart

Technical Tip:

Preparation of Gelatin Adhesive

msdsarrow12Gelatin, Powder, U.S.P.

CAS #9000-70-8
Solubility: 12% in H2O at 20°C
pH (1% in H2O): 3.5-5.0
Residue after ignition: <2%
Type A-from Porcine skin (acid cured tissue)
Type B-from Bovine skin (base cured tissue)
The strength of the gel is based on the bloom number. The higher the bloom number the stronger the gel.

  • Used as a combination Acrylamide-Gelatin-Jung resin for cryosectioning of large and soft tissues (eggs, fishes, insects) Hartmann, R. (1984). A new embedding medium for cryosectioning eggs of high yolk and lipid content. Eur. J. Cell Biol., 34:206
  • Used to prepare gelatin-chrome alum adhesive to coat glass slides for mounting semi-thin sections for staining.
RT 16560 Gelatin, Type A; × 60 Bloom msds 100g 52.00 Add to Cart
RT 16562 Gelatin, Type A; × 175 Bloom msds 100g 48.00 Add to Cart
RT 16564 Gelatin, Type A; × 300 Bloom msds 100g 48.00 Add to Cart
RT 16566 Gelatin, Type B; × 75 Bloom msds 100g 48.00 Add to Cart
RT 16568 Gelatin, Type B; × 225 Bloom msds 100g 48.00 Add to Cart

msdsHistocryl Resin arrow12Giemsa Solution, C.N.#DcGe-19

Glycerin (CAS#: 56-81-5): 61%
Methyl Alcohol (CAS#: 67-56-1): 38.3%
Azur II (CAS#: 37247-10-2): 0.16%
Azur II Eosin (CAS#: 53092-85-6): 0.06%

Specific Gravity: 0.89
Differentiates types of leucocytes, bacteria and inclusion bodies.
Giemsa, Centralbl. F. Publ., Abt. 1,37, 308 (1904) Stain for thick blood films; shows malarial parasites. Barber and Komp, Publ. Health Rep., 44, 2330 (1929) Bone marrow and stain for showing cell morphology; together with May-Grunwald Solution for pap staining. Pappenheim, Folia Haemat., 13, 338 (1912)

RT 15940 Giemsa Stain Solution 100 ml 20.00 Add to Cart

msdsarrow12Giemsa Stain, C.N.#DcGe-19, Powder

CAS #51811-82-6
See the listing in Certified Dyes/Stains

RT 15942   10g 40.00 Add to Cart

arrow12For our Giesma Stain Solution

See the listing in Histology: Certified Dyes/Stains

arrow12For our Differential Quick Staining Kit (Modified Giesma) Stain Solution

See the listing in Histology: Prepared Staining Solutions

arrowGill’s Hematoxylin Staining Solution

Gill's Hematoxylin is a specially formulated solution for use as a biological stain for the nuclei and nuclear chromatin of normal and abnormal cells whether in whole or sliced cells. Our Gill's Hematoxylin is designed to use at its full strength. It is microfiltered prior to shipment; therefore, filtration before use is not required. For repeated use further filtration is recommended. At the discretion of the user, the Gill's Hematoxylin No. 2 and No. 3 may be diluted with ethylene glycol (25% is recommended) to produce a weaker stain solution.

RT 26030-10 Gill's Hematoxylin No. 1 msds 1 L 66.00 Add to Cart
RT 26030-14 Gill's Hematoxylin No. 1 4 L 238.00 Add to Cart
RT 26030-20 Gill's Hematoxylin No. 2 msds 1 L 67.00 Add to Cart
RT 26030-24 Gill's Hematoxylin No. 2 4 L 246.00 Add to Cart
RT 26030-30 Gill's Hematoxylin No. 3 msds 1 L 72.00 Add to Cart
RT 26030-34 Gill's Hematoxylin No. 3 4 L 279.00 Add to Cart

msdsarrow12Glycerine, Reagent, A.C.S.

(Glycerol)
C3H5(OH)3   F.W. 92.10   CAS #56-81-5

Specific Gravity: 1.23    
Assay   <99.5%
Residue after Ignition   0.005%
Chlorinated Compounds(as Cl)   0.003%
Sulfate   To Pass Test
Heavy Metals (as Pb)   0.0002%
RT 16550   450 ml 37.00 Add to Cart

msdsarrow12(GMA), Glycol-Methacrylate, Low Acid

(2-Hydroxyethyl methacrylate monomer, inhibited;HEMA)
C6H10O3   F.W. 130.14   CAS#868-77-9
Specific Gravity: 1.07
b.p. 67°C
Inhibited with approximately 330ppm MEHQ
A water soluble monomer.

RT 16800   450 ml 37.00 Add to Cart

Article of Interest

Preparation of Glycol Methacrylate

GMA, Water Solublearrow12GMA Embedding Kit

Please see listing in Embedding Media Kits.

  14200   kit 50.00 Add to Cart

GMA, Water Solublearrow12GMA-PEG Embedding Kit Polyethylene Glycol- Glycol methacrylate mixture.

Please see listing in Embedding Media Kits.

RT 14250 GMA-PEG Embedding Kit consists of: kit 50.00 Add to Cart
450 ml Glycol methacrylate, GMA msds
225 ml n-Butyl methacrylate msds
50 ml Ethylene glycol dimethacrylate msds
20 ml Polyethylene Glycol 400 msds
15g Benzoyl Peroxide Paste msds

msds arrow12Gold Chloride, Hydrate(Chloroauric Acid; Hydrogen Tetrachloroaurate)

H(AuCl4)·H2O   F.W. 357.79   CAS #16961-25-4
Assay 99.9985%
Gold contents 49%+
A highly purified gold compound for the preparation of Colloidal Gold sols. Preweighed to facilitate your work. (Preparation instructions for Gold Sols included)

RT 16580   10x1/10g 92.00 Add to Cart
RT 16582   10x1/2g 420.00 Add to Cart
RT 16583   1g 89.00 Add to Cart
RT 16584   10x1g 730.00 Add to Cart

arrow12Gold Chloride Solutions

Please see listing in Reagents for Histology .

arrow12Tannic Acid, used to prepare gold sols

Please see listing in Chemicals.

msds arrowGram's Iodine Solution

For use as a bacterial, fungal and inclusion body stain.

RT 26054-05 Gram's Iodine Solution 500 ml 23.00 Add to Cart
RT 26054-06 Gram's Iodine Solution 1 L 40.00 Add to Cart

arrow12Graphite Adhesives

Please see listing in Colloidal Compounds.

arrow12Gum Mastic

Please see listing in Reagents for Histology .

arrow12HANKS's Balanced Salt Solution (HBSS)

All of our Hanks’ Balanced Salt Solutions contain: Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Use the table below as a quick reference for identifying the one that best suits your application. All solutions are prepared in 1X strength.

Ingredients F.W. Formula I Formula II Formula III
KCl 74.56 X X X
NaCl 58.44 X X X
Na2HPO4. 7H2O 268.07 X X X
D-Glusose 180.16 2 g/L 1 g/L ----
MgSO4. 7H2O 246.48 X X X
CaCl2, 2H2O 147.02 X X X
KH2PO4 136.09 X X X
NaHCO3 84.01 X X X
Phenol Red   0.02 g/L 0.02 g/L 0.015 g/L
RT 11768-05 HBSS, Formula I, 0.179M msds 500 ml 21.00 Add to Cart
RT 11768-10 HBSS, Formula I, 0.179M 1 L 26.00 Add to Cart
RT 11769-05 HBSS, Formula II, 0.155M msds 500 ml 21.00 Add to Cart
RT 11769-10 HBSS, Formula II, 0.155M 1 L 26.00 Add to Cart
RT 11770-05 HBSS, Formula III, 0.147M msds 500 ml 21.00 Add to Cart
RT 11770-10 HBSS, Formula III, 0.147M 1 L 26.00 Add to Cart

arrowHarris’ Hematoxylin

Stain selectivity for nuclei, fat and lipids. Ready-to-use.

RT 26041-05 Harris' Hematoxylin msds 500 ml 34.00 Add to Cart
RT 26041-06 Harris' Hematoxylin 1 L 58.00 Add to Cart
RT 26041-20 Harris' Hematoxylin without Mercury msds 500 ml 34.00 Add to Cart

msdsarrow12Hematoxylin, Certified, C.N.#DcH-48

(Natural Black I, Hydroxybrasilin)
C16H14O6   F.W. 302.29   CAS #517-28-2
Solubility: 10.0% Water; 10.0% Alcohol; 9.5% Cellosolve; 10% Glycol; 0.0% Xylene. A certified biological stain used in histology and cytology and plastic embedded tissues.
Chen, Chang, S., J Arch. of Path., 93,334 (1972).

RT 16620   10g 28.00 Add to Cart

Technical Tip

Hematoxylin Staining Tips

msdsarrow12Hepes 0.2M Buffer Solution

Please see listing in Prepared Buffers.

RT 11494   500 ml 21.00 Add to Cart

msdsarrow12Hepes, Crystal, Biological Buffer

(N-2-Hydroxyethylpiperazine-N'-2-Ethanesulfonic acid)
C8H18O4N2S   F.W. 238.30   CAS #7365-45-9

Assay   >99%
pKa @20°C   7.55
pH Range   7.0-8.0
A(260) (1.0M in H2O)   <0.05
Ash   <0.1%
Good's buffer.    
RT 16780   25g 14.50 Add to Cart
RT 16782   100g 52.00 Add to Cart

msdsarrow12(HMDS), Hexamethyldisilazane, Reagent

(CH3)3SiNHSi(CH3)3   F.W. 161.40   CAS #999-97-3
b.p. 126.7°C
Assay >97.0%
Specific Gravity @25°C 0.774
Refractive Index @20°C: 1.407
* For fast preparation of soft insect tissues for SEM. A good substitution for critical point drying. Int. J. Insect Morh. Embryol., 12(4), 201 (1983) Stain Tech., 58(6), 347 (1983)
* Bray, D.F., Et-al. (1993). Micros. Res. and Technique. 26:489-495. A comparison of HMDS, Peldri II, and critical point drying methods for SEM of biological specimens.

RT 16700   100 ml 18.00 Add to Cart

msdsarrow12Hexamethylenetetramine A.C.S. Reagent, Granular

(Urotropine; Methenamine)
C6N4   F.W. 140.19   CAS #100-97-0
Assay: 99.0-100.5%
Specifications:

Residue after Ignition   0.1%
Chloride   0.014%
Sulfate   To Pass Test
Heavy Metals.   0.001%

Used in silver stains.
Swift, J.A. (1968) Microsc. Soc. 88, 449.

RT 16710   50g 14.00 Add to Cart
RT 16710-25*   25g 8.50 Add to Cart

*We are now offering Hexamethylenetetramine in 25 ml bottles to avoid its classification as a hazardous chemical for shipping purposes.

msdsarrow12Hexylene Glycol, Reagent, A.C.S.

(2-Methyl-2,4-pentanediol)
(CH3)2C(OH)CH2CH(OH)CH3   F.W. 118.18   CAS #107-41-5
Specific Gravity @25°C 0.923
A.C.S. Specifications:

Assay   99%
IR Spectrum   To Pass Test
Refractive Index @20°C   0.921-0.923

Mixed with water in all proportions. Used as a dehydrating agent for fat tissues.

RT 16740   450 ml 24.00 Add to Cart
RT 16750   4x450 ml 89.00 Add to Cart

GMA, Water Solublearrow12HPMA Embedding Kit

Please see listing in Embedding Media Kits.

RT 14220   kit 42.00 Add to Cart

msdsarrow12HRP Conjugate Diluent

Specifically designed for preparing solution of your HRP-conjugate used as the detection reagent. It is the Antibody-dilutent buffer with additional component for stabilizing your HRP-conjugate. Allows you to further standardize the assay preparing ready-to-use conjugate solutions in advance and store them in refrigerator without loss of activity.

4-8°C 62716 HRP Conjugate Diluent 50 ml 102.00 Add to Cart
4-8°C 62717 HRP Conjugate Diluent 125 ml 320.00 Add to Cart
4-8°C 62718 HRP Conjugate Diluent 500 ml 1,150.00 Add to Cart

Certificates of Analysis


Hydrochloric to Mucicarmine arrow12arrow13