Hydrochloric
to Mucicarmine
Hydrochloric Acid Solution 0.1N
HCl CAS #7647-01-0
Used to adjust the pH of buffers and fixative solutions.
| RT | 16760 | 100 ml | 5.00 | Add to Cart |
Hydrochloric Acid Solution 1.0N
HCl CAS #7647-01-0
Used to adjust the pH of buffers and fixative solutions.
| RT | 16770 | 100 ml | 7.00 | Add to Cart |
Hydrochloric Acid Solutions
For all our Hydrochloric Acid Solutions, please see listing in Reagents for Histology.
Hydrogen Peroxide 30% Solution-Stabilized, Reagent, A.C.S.
H2O2 F.W. 34.01 CAS #7722-84-1
| Assay | 29-32% | |
| Maximum of Impurities: | ||
| Chloride(Cl) | 0.0003% | |
| Nitrate | 0.0002% | |
| Phosphate | 0.0002% | |
| Sulfate | 0.0005% | |
| Heavy Metal | 0.0001% | |
| Iron | 0.00005% | |
| Titratable Acid | 0.0006meq/g |
| 0-5°C | 16790 | 100 ml | 72.00 | Add to Cart | |
| 0-5°C | 16792 | 450 ml | 149.50 | Add to Cart |
2-Hydroxyethyl Methacrylate
Please see (GMA) Glycol methacrylate.
| RT | 16800 | 450 ml | 37.00 | Add to Cart |
Hydroquinone Solutions
For all our Hydroquinone Solutions, please see listing in Reagents for Histology.
(HPMA), 2-Hydroxypropyl Methacrylate
(Propylene glycol monomethacrylate)
C7H12O3 F.W. 144.17 CAS #27813-02-1
b.p. 70°C/1mmHg
Specific Gravity @25°C 1.028
Solubility in Water 13%
Inhibited with approx. 300ppm MEHQ
A water miscible monomer
| 0-5°C | 16900 | 450 ml | 30.00 | Add to Cart | |
| 0-5°C | 16901 | 4x450 ml | 108.00 | Add to Cart |
Imidazole, Reagent
(Glyoxaline)
C3H4N2 F.W. 68.08 CAS #288-32-4
Assay min 99.5%
Imidazole is a lipid-enhancing buffer agent. When used with 4% osmium tetroxide solution in the post-fixation of tissue blocks, it produces a prominent electron-opaque staining of lipid droplets with no evidence of diffusion.
Reference: Angermuller, S. Fahimi, H., Histochem. Journal 14, 823-35 (1982)
| RT | 16905 | 100g | 36.00 | Add to Cart |
EMS Immersion Oils
EMS is proud to introduce a complete new line of immersion oils. This new family includes 5 unique types for
- Critical use
- Synthetic
- High viscosity
- Low viscosity with R.I 1.480 (Synthetic)
- Low refractive index (1.455) – suitable with aqueous media
A. EMS 255 Immersion Oil
Critical use – A synthetic immersion oil - for use with oil immersion lenses in microscopy. Manufactured and tested to a tight R.I. of 1.517 ± 0.001. The color is water-white to a light straw color. Comes complete with dispenser nozzle.
| RT | 16007-01 | EMS 255 | 30 ml | 105.00 | Add to Cart |
| RT | 16007-02 | EMS 255 | 100 ml | 195.00 | Add to Cart |
| RT | 16007-03 | EMS 255 | 10 L | 905.00 | Add to Cart |
| RT | 16007-04 | EMS 255 | 25 L | 2,000.00 | Add to Cart |
B. EMS 250 Immersion Oil
A medium viscosity oil formed from long chain hydrocarbons and polybutenes and terphenyls with a R.I of 1.518 ± 0.002 with water-white to a light straw color.
| RT | 16005-01 | EMS 250 | 30 ml | 74.00 | Add to Cart |
| RT | 16005-02 | EMS 250 | 100 ml | 150.00 | Add to Cart |
| RT | 16005-03 | EMS 250 | 10 L | 650.00 | Add to Cart |
| RT | 16005-04 | EMS 250 | 25 L | 1,325.00 | Add to Cart |
C. EMS 252 Immersion Oil
A high viscosity immersion oil to allow for work with slides mounted vertically to the lens, or in situations where slides are collected over a long period prior to being observed.
Manufactured from phthalate esters with a refractive index of 1.51 and is water-white to a light straw color.
| RT | 16007-05 | EMS 252 | 100 ml | 30.00 | Add to Cart |
| RT | 16007-06 | EMS 252 | 5 L | 925.00 | Add to Cart |
D. EMS 245 Immersion Oil
A low viscosity immersion oil which is suitable with aqueous media and has a R.I of 1.455.
| RT | 16006-01 | EMS 245 | 30 ml | 8.00 | Add to Cart |
| RT | 16006-02 | EMS 245 | 100 ml | 20.00 | Add to Cart |
| RT | 16006-03 | EMS 245 | 5 L | 725.00 | Add to Cart |
E. EMS 265 Immersion Oil
A low viscosity immersion oil with a R.I of 1.480.
| RT | 16006-04 | EMS 265 | 30 ml | 72.00 | Add to Cart |
| RT | 16006-05 | EMS 265 | 100 ml | 148.00 | Add to Cart |
| RT | 16006-06 | EMS 265 | 10 L | 650.00 | Add to Cart |
| RT | 16006-07 | EMS 265 | 25 L | 1,325.00 | Add to Cart |
Immersion Oils For Normal Light Microscopy
Which Type - Which Application?
Cargille PCB-Free Immersion oils were introduced in 1972. Today, no other immersion oil meets FDA criteria. Now four standard types are available with the introduction of Type NVH, a very high viscosity oil. All Cargille Immersion Oils meet DIN 58 884 (Deutsche Institut Fur Normung e.V.)
For Normal Light Microscopy
Types A & B, with viscosities of 150 and 1250cSt respectively, meet these requirements. The greater the gap between cover glass and objective, or condenser and slide, the more desirable high viscosity becomes. For extremely large gaps, type NVH should be used. All Cargille Immersion Oils can be blended for intermediate viscosities.
37°C Immersion
Oils
A number of research projects require microscopic studies at internal body temperature. Since the index/temperature relationship is inverse, increasing temperature significantly reduces the index of the immersion oil and consequently the imagery deteriorates. This problem has been resolved by the formation of Cargille 37°C Immersion Oils, which have an index of 1.515 at 37°C.
Consideration of these oils is also suggested where stage temperatures are elevated by sub-stage illuminators and high wattage projection equipment.
Immersion Oil Type 300
Automated Hematology Systems: Use Type 300; Automated Hematology Systems depend on accurate, precisely controlled physical and optical properties of immersion oil for successful imaging and mechanical processing. Type 300 is designed and manufactured to meet the stringent requirements of this equipment, which include specialized viscosity and exacting controls for its consistency.
Immersion Oils Specifications
OPTICAL & PHYSICAL PROPERTIES
| Immersion Oil Type | A | B | 300 | |
| Refractive Index : | @23°C | @23°C | @23°C | |
| F Line (4861 A) | 1.5239 | 1.5236 | 1.5238 | |
| E Line (5461 A) | 1.5180 | 1.5180 | 1.5180 | |
| D Line (5893 A) | 1.5150 | 1.5150 | 1.5150 | |
| C Line (6563 A) | 1.5114 | 1.5115 | 1.5116 | |
| Dispersion: | ||||
| nF-nC | 0.0125 | 0.0119 | 0.0123 | |
| Abbe VD | 41.3 | 43.2 | 41.8 | |
| Abbe Ve | 41.0 | 42.8 | 41.5 | |
| Temperature Coefficient (15-35°C) | -0.00033 | -0.00031 | -0.00033 | |
| Stability (change in nD25°C after 24 hrs @ temp.) | ||||
| at 60°C: | 0 | 0 | 0 | |
| at 100°C: | 0 | 0 | 0 | |
| Fluorescence (1): Short UV: | low | low | low | |
| Fluorescence (1): Long UV: | low | low | low | |
| Color: (Gardner) | 1 | 1 | 1 | |
| Viscosity: cSt +/- 10% @ 23°C (centistokes) | 150 low |
1250 high |
300 medium |
|
| Density: @ 23°C g/cc: | .923 | .923 | .923 | |
| Density: (US) lb/gal | 7.70 | 7.70 | 7.70 | |
| Cloud Point: | <-13°C | <-13°C | <-13°C | |
| Flash Point: | 325°F | 325°F | 325°F | |
| Neutralization No.: (mg KOH/g) | 0.01 max | 0.01 max | 0.01 max | |
(1) Relative to Cedarwood Oil
| RT | 16907-01 | TYPE A | |
1 oz | 12.00 | Add to Cart |
| RT | 16907 | TYPE A | 4 oz | 26.00 | Add to Cart | |
| RT | 16907-16 | TYPE A | 16 oz | 52.50 | Add to Cart | |
| RT | 16908-01 | TYPE B | |
1 oz | 13.00 | Add to Cart |
| RT | 16908 | TYPE B | 4 oz | 26.00 | Add to Cart | |
| RT | 16908-16 | TYPE B | 16 oz | 52.50 | Add to Cart | |
| RT | 16917 | TYPE 300 | |
1 oz | 14.25 | Add to Cart |
| RT | 16917-01 | TYPE 300 | 4 oz | 28.75 | Add to Cart | |
| RT | 16917-16 | TYPE 300 | 16 oz | 61.25 | Add to Cart |
Special Immersion Oils
For Horizontal, Inverted and Inclined Instruments and Projection Equipment
Higher viscosities are generally required. Type NVH meets the requirement, having a viscosity of 21,000cST.
OPTICAL & PHYSICAL PROPERTIES
| Immersion Oil Type | 37** | NVH | OVH | |
| Refractive Index : | @37°C | @23°C | @23°C | |
| F Line (4861 A) | 1.5238 | 1.5230 | 1.5230 | |
| E Line (5461 A) | 1.5180 | 1.5178 | 1.5178 | |
| D Line (5893 A) | 1.5150 | 1.5150 | 1.5150 | |
| C Line (6563 A) | 1.5115 | 1.5118 | 1.5118 | |
| Dispersion: | ||||
| nF-nC | 0.0123 | 0.0113 | 0.0111 | |
| Abbe VD | 42.0 | 45.7 | 46.3 | |
| Abbe Ve | 41.7 | 45.4 | 46.0 | |
| Temperature Coefficient (15-35°C) | -0.00035 | -0.00034 | -0.00034 | |
| Stability (change in nD25°C after 24 hrs @ temp.) | ||||
| at 60°C: | 0 | 0 | 0 | |
| at 100°C: | 0 | 0 | 0 | |
| Fluorescence (1): Short UV: | low | low | low | |
| Fluorescence (1): Long UV: | low | low | low | |
| Color: (Gardner) | 1 | 1 | <3 | |
| Viscosity: cSt +/- 10% @ 23°C (centistokes) | 1250 high |
21,000 very high |
46,000 very high |
|
| Density: @ 23°C g/cc: | 0.917 | 0.919 | 0.918 | |
| Density: (US) lb/gal | 7.73 | 7.67 | 7.66 | |
| Cloud Point: | <-13°C | <-13°C | <-13°C | |
| Flash Point: | 325°F | 325°F | 325°F | |
| Neutralization No.: (mg KOH/gm) | 0.01 max | 0.01 max | 0.04 max | |
(1) Relative to Cedarwood Oil
**All values standardized at 37°C
| RT | 16914-01 | TYPE 37 | |
1 oz | 15.00 | Add to Cart |
| RT | 16909-04 | TYPE 37 | 4 oz | 32.00 | Add to Cart | |
| RT | 16909-01 | TYPE NVH | |
1 oz | 14.50 | Add to Cart |
| RT | 16909 | TYPE NVH | 4 oz | 32.00 | Add to Cart | |
| RT | 16918 | TYPE OVH | |
1 oz | 15.50 | Add to Cart |
| RT | 16918-01 | TYPE OVH | 4 oz | 33.25 | Add to Cart | |
| RT | 16918-16 | TYPE OVH | 16 oz | 90.25 | Add to Cart |
Immersion Oils For Fluorescence Microscopy
After years of development and field testing, we now have available two new PCB-Free Immersion Oils for Fluorescence Microscopy.
* Immersion Oil Type HF
Type DF has been discontinued by Cargille. As an alternative we are offering either a new Immersion Oil called HF which would provide low fluorescence and the correct optical properties or you can use our FF which has virtually no fluorescence but slightly less than ideal optical properties.
* Immersion Oil Type FF
Type FF has virtually no background fluorescence which makes it ideal for observing low levels of fluorescence. It is highly stable, water white, and non-hydroscopic. Type FF can be substituted for all other fluorescence microscopy immersion oils in nearly all fluorescence applications.
* Immersion Oil Type LDF
Extremely low fluorescence is achieved by Type LDF. It is the replacement of choice for the DF which is no longer being manufactured. The LDF possesses the highest resolution of the oils for fluorescence applications.
* Immersion Oil Type 37LDF
Low fluorescence immersion oil for use at 37°C. Distinct odor.
Refractive Index @ 37°C
F Line (486.1 nm) - 1.5239
E Line (546.1 nm) - 1.5181
D Line (589.3 nm) - 1.5150
C Line (656.3 nm) - 1.5115
Viscosity: 1250 cSt ± 10% @ 37°C (High)
OPTICAL & PHYSICAL PROPERTIES
| Immersion Oil | Type LDF | Type FF | Type HF | |
| Refractive Index @ 23°C: | ||||
| F Line (4861 A) | 1.5239 | 1.4850 | 1.5234 | |
| E Line (5461 A) | 1.5181 | 1.4811 | 1.5180 | |
| D Line (5893 A) | 1.5150 | 1.4790 | 1.5150 | |
| C Line (6563 A) | 1.5115 | 1.4766 | 1.5117 | |
| Dispersion: | ||||
| nF-nC | 0.0125 | 0.0084 | 0.0118 | |
| Abbe VD | 41.3 | 57.0 | 43.8 | |
| Abbe Ve | 41.0 | 56.5 | 43.6 | |
| Temperature Coefficient (15-35°C) |
-0.00036 | -0.00037 | -0.00038 | |
| Stability (change in nD25°C after 24 hrs. @ temp.) | ||||
| at 60°C: | -0.0004 | 0 | +0.0001 | |
| at 100°C: | -0.0043 | 0 | +0.0006 | |
| Fluorescence (1): | ||||
| Short UV: | very low | none | very low | |
| Long UV: | very low | none | very low | |
| Color: (Gardner) | <1 | <1 | <1 | |
| Viscosity: +/-10% @ 23°C (centistokes) | 500 medium |
170 low |
700 medium |
|
| Density: | ||||
| @ 23°C g/cc: | 0.984 | 0.877 | .9306 | |
| (US) lb/gal | 8.21 | 7.32 | 7.77 | |
| Cloud Point: | <-14°C | <-6°C | <2°C | |
| Flash Point: | 171°F | 420°F | >340°F | |
| Neutralization No.: (mg KOH/gm) | <0.01 max | 0.01 max | 0.03 max | |
*Relative to Cedarwood Oil
| RT | 16919 | Immersion Oil Type LDF | |
1 oz | 16.00 | Add to Cart |
| RT | 16919-01 | Immersion Oil Type LDF | 4 oz | 36.25 | Add to Cart | |
| RT | 16919-16 | Immersion Oil Type LDF | 16 oz | 75.00 | Add to Cart | |
| RT | 16915-01 | Immersion Oil Type HF | |
1 oz.* | 16.00 | Add to Cart |
| RT | 16915-04 | Immersion Oil Type HF | 4 oz | 34.25 | Add to Cart | |
| RT | 16916-01 | Immersion Oil Type FF | |
1 oz.* | 14.75 | Add to Cart |
| RT | 16916-04 | Immersion Oil Type FF | 4 oz | 32.25 | Add to Cart | |
| RT | 16919-15 | Immersion Oil Type 37 LDF | 1/4 oz | 11.00 | Add to Cart | |
| RT | 16919-14 | Immersion Oil Type 37 LDF | |
1 oz | 16.00 | Add to Cart |
| RT | 16919-13 | Immersion Oil Type 37 LDF | |
4 oz | 34.00 | Add to Cart |
| RT | 16919-12 | Immersion Oil Type 37 LDF | |
16 oz | 80.00 | Add to Cart |
* 1 oz. Applicator bottles have a glass ball rod in the cap.
Immuno-Bed™ Embedding Kit
Please see listing in Embedding Media Kits.
ImmunoHistoZyme™, Ready-to-Use
Description: ImmunoHistoZyme is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. ImmunoHistoZyme digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.Storage: 2-8°C. DO NOT FREEZE
Intended Use:Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: Ready-to-use ImmunoHistoZyme Reagent.
| 4-8°C | 64142-13 | ImmunoHistoZyme | 15 ml | 82.00 | Add to Cart |
| 4-8°C | 64142-14 | ImmunoHistoZyme | 100 ml | 155.00 | Add to Cart |
Indigo Carmine, Certified, BSC Code #DcI-7
(Acid Blue 74; Carmine Blue; Indigotine 1, 1A)
C16H8N2O8S2Na2 F.W.466.36 CAS #860-22-0
Dye content: approx. 85%
Solubility: 1.3% Water; 0.0% Alcohol; 4.0% Cellosolve; 2.85% Glycol; 0.0% Xylene
Shunway's stain for animal embryos. Used with Acid Fuchsin for staining Negri bodies. Stain Tech., 5, 34 (1928)
Counterstain to Hematoxylin for staining vaginal smears. Am. J. Anat., 61, 505 (1937).
| RT | 16910 | 25 g | 60.00 | Add to Cart |
Technical Tip
Achieving Maximum Contrast with Stain.
Indium Trichloride, Anhydrous
InCl3 F.W. 221.18 CAS #10025-82-8
Chloride Content: 98%
A metal stain for nucleic acid
J. Biophys Biochem Cytol, 11,257 (1961)
| RT | 16920 | 5 g | 68.00 | Add to Cart |
Ingrain Blue, BSC Code #DcAn-25
Please see Alcian Blue 8GX.
| RT | 10350 | 25 g | 232.00 | Add to Cart |
Isopropyl Alcohol, Reagent, A.C.S.
(2-Propanol)
CH3CH(OH)CH3 F.W. 60.10 CAS #67-63-0
A.C.S. Specifications:
| Color (APHA) | . | 10 |
| Density @ 25°C | . | 0.781-0.783 |
| Boiling Range (including 82.3°C) | . | 1°C |
| Titratable Acid or Base | . | 0.0001 meq/g |
| Residue after Evaporation | . | 0.001% |
| Solubility in water | . | To pass test |
| Water | . | 0.2% |
| RT | 16930 | Isopropyl Alcohol, Reagent, A.C.S. | 1 qt | 14.00 | Add to Cart |
| RT | 16931 | Isopropyl Alcohol, Reagent, A.C.S. | 4x1 qt | 50.00 | Add to Cart |
JB-4™ Embedding Kit
Please see listing in Embedding Media Kits.
Karnovsky's Fixative
Please see Paraformaldehyde-Glutaraldehyde Solution.
Technical Tip
Elimination of Section Contamination During Staining
Kinyoun's Solution
Kinyoun's carbol fuchsin staining solution for acid-fast bacteria.
| RT | 26087-05 | Kinyoun's Solution | 500 ml | 61.00 | Add to Cart |
| RT | 26087-06 | Kinyoun's Solution | 1 L | 108.00 | Add to Cart |
Lanthanum Nitrate, Hexahydrate, Reagent
La(NO3)3·6H20 F.W. 433.03 CAS #10277-43-7
| m.p. | 40°C | |
| Assay | Min 99.9% | |
| Calcium | 0.001% | |
| Magnesium | 0.0001% |
A metal stain, intracellular tracer.
- Lane, N,J. and Treherne, J.E. (1970). Lanthanum staining of neurotubules in axons from cockroach ganglia. J. Cell Sci., 7:217
- Lane, N.J. (1972) Fine structure of a lipidopteran nervous system and its accessibility to peroxidase and lanthanum. Z. Zellforsch., 131:205
| RT | 17300 | 50g | 104.00 | Add to Cart |
Lead Acetate, Trihydrate Reagent, A.C.S.
Pb(CH3COO)2·3H20 F.W. 379.33 CAS #6080-56-4
A.C.S. Specifications:
| Assay | . | 99.0-103.0% |
| Chloride | . | 5ppm |
| Copper | . | 0.002% |
| Insoluble Matter | . | 0.01% |
| Iron | . | 0.001% |
| Nitrate and Nitrite (as NO3) | . | To pass test |
| 0-5°C | 17600 | 100g | 25.00 | Add to Cart | |
| 0-5°C | 17600-25 | 25g | 10.00 | Add to Cart | |
*We are now offering Lead Acetate in 25 ml bottles to avoid its classification as a hazardous chemical for shipping purposes.
Lead Citrate, Trihydrate
Pb(C6H5O7)2·3H2O FW 1053.82 CAS #512-26-5
Minimum assay 99.%
The most widely used metal stain for ultra thin sections.
Reynolds, E.S., J. Cell Bio., 17, 208 (1963)
Sato, T., J. Electron Micro., 116, 133 (1976)
Venable, J.H. and Coggeshall, R. (1965). A simplified lead citrate stain for use in electron microscopy. J. Cell Biol., 25:407
Famy, A. (1967). An extemporaneous lead citrate for electron microscopy. Proc. 25th Ann. EMSA Meet., p.148. Claitor's Pub. Division, Baton Rouge, LA.
| RT | 17800 | 25g | 28.00 | Add to Cart | |
| RT | 17810 | 100g | 72.00 | Add to Cart |
Technical Tips
Handling of Lead Solutions
Lead Staining
Common Lead Stock Solutions
A Stable Lead Staining Solution
Lead Nitrate, Reagent, A.C.S.
Pb(NO3)2 F.W. 331.21 CAS #10099-74-8
| Minimum Assay | 99.5% | |
| Insoluble matter | 0.005% | |
| Reaction | Not less than pH 3.5 | |
| Chloride | 0.0005% | |
| Calcium | 0.01% | |
| Copper | 0.0005% | |
| Iron | 0.0005% | |
| Potassium | 0.002% | |
| Sodium | 0.002% |
A metal stain for ultra thin sections.
Sato, T., (1967) J. Electron Micro., 16, 733
| RT | 17900 | 25g | 14.00 | Add to Cart |
Light Green SF Yellowish, Certified, BSC Code #DcL-38
(Acid Green 5; Light Green 2G, S or 2GN; Acid Green F; Fast Acid Green N)
C37H34N2Na2O9S3 F.W. 792.86 CAS #5141-20-8
Solubility: 20.35% Water; 4.0% Alcohol; 5.25% Cellosolve™; 12.0% Glycol; 0.0% Xylene
| RT | 17920 | 10 g | 17.00 | Add to Cart |
Light Green SF Yellowish Solution
| RT | 26085-05 | Light Green SF Yellowish, 0.2% in 0.2% Acetic Acid |
500 ml | 33.00 | Add to Cart |
| RT | 26085-06 | Light Green SF Yellowish, 0.2% in 0.2% Acetic Acid |
1 L | 62.00 | Add to Cart |
| RT | 26085-10 | Light Green SF Yellowish, 1% Aqueous |
500 ml | 42.00 | Add to Cart |
(SPURR), Low Viscosity, Embedding Kit
Please see listing in Embedding Media Kits.
| RT | 14300 | Low viscosity kit (Dr. Spurr) | |
kit | 74.00 | Add to Cart |
Technical Tips
Embedding Resin Stain Permability
Lowicryl®, Embedding Kit
Please see listing in Embedding Media Kits.
| RT | 14330 | Lowicryl® K4M | |
kit | 578.00 | Add to Cart |
| RT | 14340 | Lowicryl® HM20 | |
kit | 600.00 | Add to Cart |
| RT | 14350 | Lowicryl® K11M | |
kit | 588.00 | Add to Cart |
| RT | 14360 | Lowicryl® HM23 | |
kit | 588.00 | Add to Cart |
Technical Data Sheets and Newsletter
Lowicryl® MonoStep™ Single Component Embedding Media
The same as our Lowicryl complete kits but this new formulation allows you one stop embedding without all of the cumbersome preparation and mixing. This pre-mixed, ready to use resin, saves you time and minimizes chemical contact. MonoStep Lowicryl is ideal for use in immunohistochemistry and immunolabeling. Store at room temperature.
| RT | 14335 | Lowicryl® K4m Polar Embedding Medium; MonoStep™ |
|
225 g | 264.00 | Add to Cart |
| RT | 14345 | Lowicryl® HM-20 Polar Embedding Medium; MonoStep™ |
|
225 g | 268.00 | Add to Cart |
LR White And LR Gold Resin
Please see listing in Embedding Media Kits.
Articles of Interest
Lucifer Yellow, CH, Dilithium Salt
C13H9Li2N2O9S2 F.W. 457.25 CAS #67769-47-5
A technique producing an electron dense osmiophilic polymer when Lucifer Yellow is injected into cells. Lucifer Yellow also can be viewed in living cells as a dye marker and is more sensitive than any other dye.
Cell Tissue Res., 204, 37 (1979) J. Histochem Cystochem, 30, 189 (1982) Science, 217, 953 (1982); 215, 1641 (1982); 214, 801 (1981); 225, 851 (1984); Cell, 14, 741 (1978). A fluorescent dye. Nature 292, 17 (1981)
| 0-5°C | 17950 | 25 mg | 158.00 | Add to Cart |
Lugol's Iodine
For use as a bacterial, fungal and inclusion body stain.
| RT | 26055-05 | Lugol's Iodine Solution | 500 ml | 23.00 | Add to Cart |
| RT | 26055-06 | Lugol's Iodine Solution | 1L | 44.00 | Add to Cart |
Magnesium Chloride, Hexahydrate, Reagent, A.C.S.
MgCl2·6H2O F.W. 203.30 CAS #7791-18-6
Assay: 99.0-102.0%
| RT | 18010 | 100 g | 22.00 | Add to Cart |
Malachite Green 4, Certified C.N.#DcMg-11
Please see Basic Green Oxalate.
| RT | 18100 | 25 g | 42.00 | Add to Cart |
Maleic Acid, Crystalline, Repurified, A.C.S.
(Toxilic Acid; cis-Butenediodic Acid)
HOOCCH:CHCOOH F.W. 116.08 CAS #110-16-7
m.p. 132-134°C
Assay 99.5%
| Insoluble Matter | 0.005% | |
| Fumaric Acid | 0.15% | |
| Chloride | 0.01% | |
| Sulfate. | 0.001% |
| RT | 18150 | 250 g | 40.00 | Add to Cart |
Mammalian Ringer Solution
Ingredients: NaCl; KCl; CaCl2·6H2O; NaHCO3; MgCl2·6H2O; Glucose and Distilled water.
| RT | 11763-05 | Mammalian Ringer Solution | 500 ml | 27.00 | Add to Cart |
| RT | 11763-10 | Mammalian Ringer Solution | 1 L | 35.00 | Add to Cart |
May-Grunwald Staining Solution
A ready-to-use stain for bone marrow staining.
| RT | 26064-05 | May-Grunwald Stain Solution | 500 ml | 36.00 | Add to Cart |
| RT | 26064-06 | May-Grunwald Stain Solution | 1 L | 64.00 | Add to Cart |
Mayer's Hematoxylin
Stain selectivity for nuclei. Ready-to-use.
| RT | 26043-05 | Mayer's Hematoxylin | 500 ml | 50.00 | Add to Cart |
| RT | 26043-06 | Mayer's Hematoxylin | 1 L | 92.00 | Add to Cart |
| RT | 26043-10 | Mayer's Hematoxylin | 4 L | 315.00 | Add to Cart |
Modified Mayer's Hematoxylin
Stock Solution. Ready-to-use.
Ingredients: Hematoxylin 0.4%, Ammonium Aluminum Sulfate 0.5%,
Sodium Iodate: 0.03%, Citric Acid 0.1%, Chloral Hydrate 5%
| RT | 26043-18 | Modified Mayer's Hematoxylin | 500 ml | 46.00 | Add to Cart |
Methacrylic Acid, Glacial
(2-Methylpropenoic Acid)
CH2:C(CH3)COOH F.W. 86.09 CAS #79-41-4
Assay: >98%
Stab. with 0.25% Hydroquinone monomethyl ether
| RT | 18500 | 450 ml | 39.50 | Add to Cart |
Methanol, Reagent, A.C.S.
(Methyl Alcohol)
CH3OH F.W. 32.04 CAS #67-56-1
Assay: 99.8%
Specific Gravity : 0.79
A.C.S. Specifications:
| Residue after Evaporation | . | 0.001% |
| Boiling Range (Boiling point 64.6°C) | . | 2°C max |
| Appearance | . | Clear |
| Color (A.P.H.A.) | . | 10 max |
| Acetone & Aldehydes (0.001%) | . | To pass test |
| Titratable Acid | . | 0.0003 meq/g |
| Titratable Base | . | 0.0002 meq/g |
| Water | . | 0.2% max |
| RT | 18510 | 1 qt | 16.00 | Add to Cart | |
| RT | 18511 | 4x1 qt | 58.00 | Add to Cart |
Methenamine
Please see Hexamethylenetetramine.
| RT | 16710 | 50 g | 14.00 | Add to Cart |
Methenamine Solution
A stock solution for use in bacterial and fungal stains (Grocott's Method/GMS).
| RT | 26083-05 | Methenamine, 3% Aqueous Solution | 500 ml | 26.00 | Add to Cart |
| RT | 26083-06 | Methenamine, 3% Aqueous Solution | 1 L | 46.00 | Add to Cart |
2-Methyl Butane, Reagent
(Isopropentane)
CH2H5CH(CH3)2 F.W. 72.15 CAS #78-78-4
b.p. 27-31°C
Specific Gravity @ 20°C: 0.62
| RT | 18550 | 450 ml | 45.00 | Add to Cart |
Methyl Cellulose, 400 cPS,USP/NF
(Methylcel MC; Cellulose Methyl Ether)
CAS #9004-67-5
Methoxyl (-CH3) content : 27.5-31.5%
Medium viscosity (2% in water, @20°C): 230-550 cps
Used with polyethylene glycol in the presence of acidic uranyl acetate to stain cryo sections.
| RT | 18560 | 100 g | 50.00 | Add to Cart |
MethyleneBlue Chloride, BSC Code #DcA-37
(Basic Blue 9; Swiss Blue)
C16H18N3SCl •3H2O F.W. 319.86+aq CAS #7220-79-3
Solubility: 9.5% Water; 6.0% Alcohol; 6.5% Cellosolve™; 10.0% Glycol; 0.0% Xylene.
- Used to distinguish autonomic nerves. Richardson, K.C., (1969) Anat. Rec., 164, 231. General stain for animals, histology.
- A simple rapid staining procedure for methacrylate embedded tissue sections using chromotrope 2R and Methylene Blue. Dougherty, M.M., and King, J.S., (1984) Stain Tech., 59, 149
- An established routine method for differential staining of epoxy-embedded tissue sections. Schroeder, H.E., Rossinsky, K., and Muller, W., (1980) Microsc. Acta 83, 111
- Used with Basic Fuchsin for stain epoxy-embedded tissue. Sato, T. and Shamoto, M. (1973). A simple rapid polychrome stain for epoxy-embedded tissue. Stain Technol., 48:223
| RT | 18600 | 25 g | 39.00 | Add to Cart |
Technical Tips
A Methylene Blue-Azure II Stain
Methylene Blue Solutions
Please see listing in Prepared Staining Solutions
Methyl Ethyl Ketone, Reagent
(2-Butanone)
CH3COCH2CH3 F.W. 72.11 CAS #78-93-3
| Assay | >98% | |
| Specific Gravity (H2O=1) : | 0.799-0.802 | |
| Acidity (as CH3COOH) | 0.003% | |
| Water | 0.2% |
| RT | 18650 | 450 ml | 34.00 | Add to Cart |
Methylene Chloride, Reagent, A.C.S.
(Dichloromethane; Plastisolve)
CH2Cl2 F.W. 84.93 CAS #75-09-2
| RT | 19600 | 1qt | 39.75 | Add to Cart | |
| RT | 19610 | 4x1qt | 150.00 | Add to Cart |
Technical Tips
Methyl Green, Certified, BSC Code #DcG-20
(Zinc Chloride Salt; Ethyl Green)
C27H35N3C12·ZnC12 F.W. 608.79 CAS #7114-03-6
Solubility: 8.0% water; 3.0% Alcohol; 1.8% Cellosolve; 3.5% Glycol; 0.0% Xylene.
- Used as a counterstain in Almann's aniline acid fuchsin technique for mitochrondria; used in Kurnick's Methyl Green-Pyonin stain for nuclei and cystoplasmic granules of liver cells. Marker for DNA at acid and neutral conditions. J.B. Boyd, H.R. Mitchell, (1965) Anal. Biochem., 13, 28.
- Used for epoxy-embedded thick sections. Sievers, J. (1971).
- Basic two-dye stains for epoxy-embedded 0.3-1 micron sections. Stain Technol., 46:195
| RT | 18700 | 25g | 60.00 | Add to Cart |
Methyl Methacrylate, Monomer
(Methacrylic Acid Methyl Ester)
CH2·C(CH3)COOCH3 F.W. 100.12 CAS #80-62-6
b.p. 99-100°C
Specific Gravity @20°C : 0.943
Inhibitor (Hydroquinone): 0.0025% (25ppm)
| 0-5°C | 18800 | 225 ml | 9.00 | Add to Cart |
Methyl Methacrylate/Butyl Methacrylate Embedding Kit,
Please see listing in Embedding Media Kits
Methyl Violet 2B, Certified, BSC Code #DcMv-10
(Basic Violet 1; Dahlia B; Gentian Violet; Paris Violet; Pyoktanium Coeruleum)
C24H28N4Cl F.W. 393.96 CAS #8004-87-3
Solubility: 9.0% Water; 8.25% Alcohol; 9.0% Cellosolve™; 8.5% Glycol;0.01% Xylene
Dye content: 75% min
Used with Methyl Green to stain Epoxy-embedded sections.
| RT | 18850 | 25g | 48.00 | Add to Cart |
Michel's Fixative
Please see listing in Reagents for Histology
Microbed Embedding Kit
Please see listing in Embedding Media Kits
Millonig's Phosphate Buffer
Millonig (1964); Karlsson and Schultz (1965)
Ingredients:
Sodium Phosphate, monobasic (NaH2PO4xH2O)
Sodium Phosphate, dibasic (Na2HPO4xH2O)
0.5% Sodium Chloride (NaCl)
Shipping Conditions: Ambient Temperature
The pH of this buffer is 7.4 (0.1M); the osmolarity is 440 mosmols, so this buffer is hypertonic to most body fluids. Millonig recommended to use this buffer for the fixation of very hydrated tissues with osmium tetroxide. For marine organisms, a higher concentration of sodium chloride (3%) is recommended. We supply it in a double strength (0.2M), pH 7.4. Dilution is needed before use.
| 2-8°C | 11582-05 | Millonig's Phosphate Buffer 0.2M | 500 ml | 23.50 | Add to Cart |
| 2-8°C | 11582-10 | Millonig's Phosphate Buffer 0.2M | 1 L | 33.00 | Add to Cart |
| 2-8°C | 11582-10-1G | Millonig's Phosphate Buffer 0.2M | 1 Gal. | 120.00 | Add to Cart |
| 2-8°C | 11582-10-5G | Millonig's Phosphate Buffer 0.2M | 5 Gal. | 525.00 | Add to Cart |
Molybdic Acid, Ammonium Salt
Please see ammonium molybdate
| RT | 10500 | 100g | 70.00 | Add to Cart |
MOPS, Biological Buffer
(3-(N-Morpholino) propanesulfonic acid)
C7H15NO4H F.W.209.26 CAS #1132-61-2
Assay: >99.%
| RT | 18900 | 100g | 74.00 | Add to Cart |
Mucicarmine/Metanil
For use with hematoxylin and metanil yellow for the determination of carbohydrates and mucosubstances.
| RT | 26080-05 | Mucicarmine Stock Solution | 500 ml | 64.00 | Add to Cart |
| RT | 26080-06 | Mucicarmine Stock Solution | 1 L | 110.00 | Add to Cart |
| RT | 26081-05 | Metanil, 0.25% Stock Solution |
500 ml | 27.00 | Add to Cart |
| RT | 26081-06 | Metanil, 0.25% Stock Solution | 1 L | 46.00 | Add to Cart |
