Tannic Acid to Z6040
C76H52O46 F.W. 1701.28 CAS #1401-55-4
|RT||21700||100 g||13.00||Add to Cart|
Gallotannin; Chiefly (C14H10O9)n F.W. range 1000.00-1500.00 CAS #1401-55-4
Low Molecular Weight. Suitable for electron microscopy
- Takagi, M., Parmley, R.T., Denys, F.R. and Kageyama, M. (1983). Ultrastructural visualization of complex carbohydrates in epiphyseal cartilage with the tannic acid-metal salt methods. J. Histochem. Cytochem.,31:783
- Simionescu, N. el al., J. Cell Biol., 70:608 (1976) A Combination of Tannic acid Glutaraldehyde Osmium Tetroxide (TAGO) Method. improving membranes contrast.
- Roubos, E. W. and Van DerWal-Divendel, R.M. (1980). Ultrastructural analysis of peptide-hormone released by exocytosis. Cell Tiss. Res., 207:267
- Buma, P.,Roubos, E.W. and Buijs, R.M. (1984). Ultrastructural demonstration of exocytosis of neural, neuroendocrine, and endocrine secretions with an in vitro tannic acid (TARI) method. Histochemistry, 80:247
- Buma, P. and Nieuwenhuys, R. (1987). Ultrastructural demonstration of oxytocin and vascopressin release sites in the neural lobe and median eminence of the rat by tannic acid and immunogold methods. Neurosci. Lett., 74:151
- Brooks, J.C. and Carmicheal, S.W. (1987). Ultrastructural demonstration of exocytosis in intact and saponin-permeabilized cultured bovine chromaffin cells. Am. J. Anat., 178:85
|RT||21710||100g||62.00||Add to Cart|
Tannic Acid Solutions
Please see listing in Reagents for Histology.
Technovit: Glycol Methacrylate Embedding Kits
C16H20N2 F.W.240.35 CAS
A non-carcinogenic substitute for DAB and DAB-HCl. Used as a sensitive and specific reagent for the detection of blood.
- Standefer, J.C., Vanderjagt, D. (1977) Assay of Hemoglobin. Clin. Chem. 23, 749.
- Boss, E.S., et al., (1981). Assay of Peroxidases. J. of Immunoassay. 2,187.
- Holland, V.R., et al., (1974) Tetrahedron, 30, 3299.
|0-5°C||13070||1g||27.00||Add to Cart|
C16H20N2·2HCl F.W. 313.30 CAS #64285-73-0
Assay >98% (Cl)
|0-5°C||13074||TMB-HCl||1gm||89.00||Add to Cart|
Also available in tablet form. 1mg/ tablet, ready to use.
|0-5°C||13075||TMB-HCl tablets||10/bottle||32.00||Add to Cart|
|0-5°C||13076||TMB-HCl tablets||50/bottle||140.00||Add to Cart|
[H2C=C9CH3)CO2CH2CH2] 2 F.W. 226.68
Specific Gravity (H2O=1) : 1.023
b.p. @4 mmHg 132-134°C
Inhibited with 200 ppm hydroquinone monomethyl ether.
|RT||21750||100 ml||18.00||Add to Cart|
Si(CH3)4 M.W. 88.22
An organo-silicon compound tetramethylsilane (TMS) is used as an internal standard for nuclear magnetic resonance (NMR). It is chemically inert and has a boiling point of 26.6°C which is lower than acetone (53.6°C), and other highly volatile liquids, such as diethyl ether (34.5°C) and 1,2-epoxy propane (36.4°C). It is soluble in most organic solvents but insoluble in water. All of these properties make TMS an ideal solvent for tissue drying, which preserves excellent tissue surface details. Dey, Sudip. Et al. (1989). A new rapid method of air-drying for scanning electron microscopy using tetramethylsilane. J. of Microscopy, Vol 156, pp. 259-261.
|RT||21760||100 ml||185.00||Add to Cart|
C42H32Cl2N12O6S2 F.W. 935.8 CAS #36889-43-7
|RT||21800||100mg||11.00||Add to Cart|
Please see Nitro-Blue Tetrazolium.
H2NNHCSNHNH2 F.W. 106.15 CAS
b.p. 171°C (decomposed)
An indefinite shelf life due to a special purification process.
- Seligman A.M. et al (1965).Histochemical demonstration of some oxidized macromolecules with thiocarbohydrazide (TCH) or thiosemicarbazide (TSC) and osmium tetroxide. J. Histochem. Cytochem., 13:629
- Seligman, A.M. et al (1966). A new staining method (OTO) for enhancing contrast of lipid-containing membranes and droplets in osmium tetroxide fixed tissue with osmiophilic thiocarbohydrazide (TCH). J. Cell Biol., 30:424
- Thiery, J.P. (1967). Mise en evidence des polysaccharides sur coupes fines en microscopie electronique. J. Microsc., 6:987
- Lo, H.K. et al (1987). A modified periodic acid-thiocarbo-hydrazide-silver proteinate staining sequence for enhanced contrast and resolution of glycogen depositions by transmission electron microscopy. J. Histochem. Cytochem., 35:393
- Neiss, W.F. (1988). Enhancement of the periodic acid-Schiff (PAS) and periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) reaction in LR White sections. Histochemistry, 88:603
- Hanker, J.S. et al (1964). Osmiophilic reagents: new cytochemical principles for light and electron microscopy. Science, N.Y., 146:1039
- Derenzini, M., et al (1986). An improved periodic acid-thiosemicarbazide-osmium technique to reveal glyco-conjugates at the molecular level in situ. J. Histochem. Cytochem.,34:1161
|RT||21900||5g||80.00||Add to Cart|
NH2CSNHNH2 F.W. 91.14 CAS
References for use-see Thiocarbohydrazide
|RT||22000||25g||12.00||Add to Cart|
Tissue Marking Dyes
C6H5CH3 FW 92.14 CAS #108-88-3
Specific Gravity (H2O=1): 0.866
|Residue after evaporation||0.001%|
|Subs. darkened by H2SO4||To pass test|
|Sulfur compound (as S)||0.003%|
|RT||22030||450 ml||10.50||Add to Cart|
|RT||22032||4x450 ml||38.00||Add to Cart|
(Basic blue; Methylene Blue T50 or T Extra)
C15H16N3SCl FW 305.83 CAS #92-31-9
Dye Content: approx. 85% min.
Solubility: 3.25% Water; 1.75% Alcohol; 3.5% Cellosolve; 5.5% Glycol; 0.0% Xylene
*-A general stain for epoxy thick sections. Barajas et al.(1981) Identification of renal neuroeffector junctions by electron microscopy of reembedded light microscopic autoradiograms of semithin sections. J. Ultrastruct. Res. 77: 379.Campbell, R.D., And Hermans, C.O. (1972). A rapid method for resectioning 0.5 4.0 micron epoxy sections for electron microscopy. Stain Technol. 47:115.
|RT||22050||50g||36.00||Add to Cart|
(CH3C6H4O)3PO F.W. 368.37 CAS #1330-78-5
Specific Gravity (H2O=1): 1.16
A mixture of isomeric Tritolyl Phosphates, usually excluding the very toxic group ortho-isomer. Used to prepare benzoyl peroxide paste, which acts as a catalyst for water miscible resins.
|RT||22150||225 ml||22.00||Add to Cart|
Please see DMP-30.
|RT||13600||25 ml||3.50||Add to Cart|
Please see Picric Acid .
|RT||19550||100g||94.00||Add to Cart|
White crystalline powder
C4H11NO3 F.W. 121.14 CAS #77-86-1
A 40% (w/v) aqueous solution. It is clear and colorless.
|RT||11700||100g||17.00||Add to Cart|
|RT||11720||500g||49.00||Add to Cart|
|RT||11730||250 ml||21.00||Add to Cart|
|RT||11740||500 ml||28.00||Add to Cart|
Storage: 2-8°C. DO NOT FREEZE
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 10 antigen retriever solution.
Preparation of Working Solution: Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C.
|4-8°C||64142-11||Tris Buffer pH 10.0||100 ml||75.00||Add to Cart|
|4-8°C||64142-12||Tris Buffer pH 10.0||1000 ml||145.00||Add to Cart|
(Polyethylene glycol tert-octylphenyl ether)
Specific Gravity (H2O=1): 1.065
|RT||22140||100 ml||10.00||Add to Cart|
|RT||22142||500 ml||39.00||Add to Cart|
Prepared 1% Triton X-100 in Deionized-Distilled water. Used for whole-cell preparations. Marek, L.F., and Kelly, R.O. (1983). A simple technique for the visualization of whole mount cytoskeletons with transmission electron microscopy. Anat. Rec. 207, 365. Pudney, J., and Singer, R.H. (1980). Intracellular filament bundles in whole mounts of chick and human myoblasts extracted with Triton X-100. Tissue cell 12, 595.
|RT||22145||500 ml||41.00||Add to Cart|
A prepared solution for the cleaning of diamond knives.
|RT||22146||500 ml||39.00||Add to Cart|
Extract from Porcine Pancreas.
Activity >100 NF units/mg
Used for whole cell preparations. (see references in Triton X-100)
|RT||22200||25g||22.00||Add to Cart|
Storage: 2-8°C. DO NOT FREEZE
Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: This enzyme is supplied as concentrated form along with buffer. The kit comes in 2 sizes:
Small: Reagent B (Buffer) is 15 ml and the Reagent E (Enzyme) is 2 ml
Large: Reagent B (Buffer) is 100 ml and the Reagent E (Enzyme) is 10 ml
|2-8°C||64142-01||Trypsin Kit Small||kit||75.00||Add to Cart|
|2-8°C||64142-02||Trypsin Kit Large||kit||145.00||Add to Cart|
H4[SiO4.(W3O9)4]·xH2O M.W. 2878.29+aq CAS #11130-20-4
|RT||22240||25g||62.00||Add to Cart|
Ingredients: NaCl; KCl; CaCl2.6H2O; MgCl2.6H2O; NaHCO3; NaH2PO4; Glucose; and Distilled water.
|RT||11760-05||Tyrode Solution||500 ml||24.00||Add to Cart|
|RT||11760-10||Tyrode Solution||1 L||33.00||Add to Cart|
|RT||14660||kit||300.00||Add to Cart|
UO2(OCOCH3)2·2H2O F.W. 424.14
|Alkalies (as SO4)||0.05%|
A universal EM stain for thin sections, en-block staining, and negative staining.
|RT||22400||25g||155.00||Add to Cart|
Substitute for Uranyl Acetate
EMS is proud to introduce UranyLess, a new contrast stain solution for TEM, for all of your negative staining applications. It is an amazing substitute for Uranyl Acetate with similar results.
After only a minute of contact, UranyLess' fast-acting, non-radioactive lanthanide mix is finished staining your sections or deposits (see protocols below). If needed, lead citrate is recommended to increase the contrast. TIP: To prevent precipitates, use room temperature distilled water.
UranyLess's pH level is about 6,8 to 7. The 30ml airless bottle will stain approximately 1500 grids. The airless bottle increases the shelf life, eliminates CO2 contamination, and produces less waste - the solution pumps out in perfect amounts without leaking or spilling. UranyLess is also available in a larger amount for use in automated staining equipment. When using UranyLess for automated staining, do not wash longer than 10 minutes or you run the risk of losing all contrast.
UranyLess has been tested on many biological tissue (animal and plant): intestine, skeletal and cardiac muscle, liver, kidney, adrenal gland, nerve, cell culture, plant tissue, and also on negative staining of bacteriophage, bacteria, and polymers. UranyLess is ideal because of its ability to stain any kind of material and results are reproducible. To see the effectiveness of Uranyless, please visit the UranyLess Image Gallery.
UranyLess has a strong contrasting power, however, we recommend a Lead Citrate counterstain to enhance the contrast. You can follow the protocol of the UranyLess / Lead Citrate double contrast by watching the video. EMS recommends Lead Citrate, Ready to use, available in a 30 ml Airless bottle. The special pump delivers the product without letting in air, thus preserving the solution and preventing CO2 dissolution. It is convenient to use because it performs in any position, even upside down.
"Easier and Safer Biological Staining: High Contrast UranyLess Staining of TEM Grids using mPrep/g Capsules." Benmeradi N1,2, Payre B2 and Goodman SL3.
- Delta Microscopies, 22, B route de saint Ybars, La côte blanche, 31190, Mauressac, France
- Université Toulouse, CMEAB Faculté Medecine, 118 route Narbonne, 31062, Toulouse, France
- Microscopy Innovations LLC, 213 Air Park Rd, Suite 101, Marshfield, WI, 54449, USA
"C-Nap1 mutation affects centriole cohesion and is associated with a Seckel-like syndrome in cattle." Nature Communications. Published 23 Apr 2015. Sandrine Floriot, all.
|RT||22409||UranyLess EM Stain*||30 ml||50.00||Add to Cart|
|RT||22409-20||UranyLess EM Stain||200 ml||225.00||Add to Cart|
|RT||22410||Lead Citrate*, Ready to use||30 ml||40.00||Add to Cart|
A non-radioactive substitute for uranyl acetate with comparable results. Avoids the tedious authorization, shipping and disposal issues involving uranyl acetate without sacrificing performance. Two lanthanide salts, samarium and gadolinium triacetate, effectively used for staining plastic-embedded animal and plant tissues. Also for negative staining of macromolecules. Not recommended as a fixative.
|RT||22405||UAR-EMS Stain||25 ml||70.00||Add to Cart|
IBI Blue Platinum Stain Pt4N8H6O24C20
An EM stain that may have the ability to replace Uranyl Acetate in some instances. If you have issues with having Uranium salts that are radioactive (depleted or not) in the lab this may be the answer for you. An alternative for staining TEM thin sections
|RT||22407||Platinum Blue||1 ml||225.00||Add to Cart|
We are now offering Uranyl Acetate pre-made and ready to use solution for making your work easier. The following percentages are avaialble off the shelf (1, 2, 3, 4). Other percentages are avaialble upon request.
|RT||22400-1||1% Uranyl Acetate Solution||100 ml||36.00||Add to Cart|
|RT||22400-2||2% Uranyl Acetate Solution||100 ml||43.00||Add to Cart|
|RT||22400-3||3% Uranyl Acetate Solution||100 ml||62.00||Add to Cart|
|RT||22400-4||4% Uranyl Acetate Solution||100 ml||74.00||Add to Cart|
For ultra fine grain structure, our Uranyl Formate offers amazing advancement in negative staining for microscopy.
|RT||22450||Uranyl Formate||1 gram||102.00||Add to Cart|
|RT||22451||Uranyl Formate||5 grams||490.00||Add to Cart|
CAS # 20596-93-4
Used as a substitute for uranyl acetate in either double-staining techniques and also as a solo stain.
|RT||22500||Uranyl Magnesium Acetate||5 grams||190.00||Add to Cart|
|RT||22501||Uranyl Magnesium Acetate||1 gram||40.00||Add to Cart|
Reagent Grade, A.C.S.
|Alkalies % Alkaline Earths (Sulfates)||<0.1%|
|Heavy Metals (as Pb)||<0.002%|
|Substances Reducing Permanganate (as UIV)||<0.06%|
A Universal EM Negative Stain used for viruses. In tissue samples it stabilizes nucleic acids and cell membranes. These solutions are more stable than uranyl acetate and they react primarily with negatively charged groups in the absence of phosphate ions.
|RT||22600||Uranyl Nitrate||5 grams||120.00||Add to Cart|
(COO)2 UO2 ·.3H2O
To adjust the pH of Uranyl compounds as well as a negative stain for electron microscopy.
|RT||22510||Uranyl Oxalate||1 gram||225.00||Add to Cart|
CAS # 10138-94-0
Used as a laboratory reagent in the determination of sodium concentrations of solutions.
|RT||22610||Uranyl Zinc Acetate||5 grams||190.00||Add to Cart|
To use with Weigert's Iodine or Weigert's Iron as a connective tissue stain.
|RT||26046-05||Van Gieson's Solution||500 ml||32.00||Add to Cart|
|RT||26046-06||Van Gieson's Solution||1 L||54.00||Add to Cart|
H2O F.W. 18.02 CAS #7732-18-5
EMS Reagent Grade Water is typically prepared at 18 megohm/cm specific resistance using a reverse osmosis, mixed deionization, activated filtration and final filtration at 0.2 microns.
Color (APHA) <+/-5
Bacteriological purity 0 CFUs/L
Residue after evaporation 10ppm
|RT||22800-01||Deionized Water||1 gal||10.00||Add to Cart|
|RT||22800-05||Deionized Water||5 gal||42.00||Add to Cart|
A histological stain. Demonstration of connective tissue is useful in cases of emphysema, arteriosclerosis and other vascular diseases
|RT||26045-05||Weigert's Iodine Solution||500 ml||32.00||Add to Cart|
|RT||26045-06||Weigert's Iodine Solution||1 L||57.00||Add to Cart|
Stain selectivity for nuclei, fungal, and general applications. Ready-to-use.
|RT||26044-05||Weigert's Iron Hematoxylin A||500 ml||38.00||Add to Cart|
|RT||26044-06||Weigert's Iron Hematoxylin A||1 L||71.00||Add to Cart|
|RT||26044-15||Weigert's Iron Hematoxylin B||500 ml||28.00||Add to Cart|
|RT||26044-16||Weigert's Iron Hematoxylin B||1 L||48.00||Add to Cart|
Solubility: 0.091% Water; 0.23% Alcohol
|RT||22720||25 g||28.00||Add to Cart|
For use as a blood stain. Ready-to-use.
|RT||26060-05||Wright Stain Solution||500 ml||32.00||Add to Cart|
|RT||26060-06||Wright Stain Solution||1 L||59.00||Add to Cart|
C6H4(CH3)2 FW 106.27 CAS #1330-20-7
Specific Gravity (H2O=1): 0.865
This reagent is a mixture of isomers (ortho, meta, and para) and may contain Ethylbenzene.
|Subs. darkened by H2SO4||To pass test|
|Sulfur Compounds (as S)||0.003%|
|Residue after evaporation||0.002%|
|RT||23400||450 ml||8.00||Add to Cart|
|RT||23402||Xylene:Peanut oil, 2:1||500 ml||43.00||Add to Cart|
A xylene substitute for use in histology and cytology. It is safer and a more preferable alternative to xylene. It can be used as a solvent and clearing agent, as well as for dissolving paraffin waxes, glues and adhesives.
- Minimal tissue shrinkage.
- Dries slowly with no residue.
- Less toxic than xylene.
- Soluble with alcohol, paraffin embedding media, and mounting media.
It can be used in all procedures that require xylene. Biodegradable.
|RT||23410-01||Xylene Substitute||1 gal||102.00||Add to Cart|
|RT||23410-04||Xylene Substitute||4x1 gal||375.00||Add to Cart|
|RT||23410-05||Xylene Substitute||5 gal||390.00||Add to Cart|
An intensive study from 1992 throughout 2000 has shown that Hemo-De® (d-Limonene) a terpene-based chemical with a pleasant citrus fragrance, was able to replace many of the clinical laboratories toxic reagents, such as carbol-xylene, xylene, ethyl acetate and formalin.
In Parasitology – Hemo-De replaces ethyl acetate in the concentration procedure; Hemo-De replaces carbol-xylene, xylene and formalin in the trichrome procedure.
In Histology – Hemo-De replaces xylene in most all procedures. Hem-De is a superior solvent and Clearing agent that can be used in all tissue processing, deparaffiniating and slide preparation.
- Soluble with alcohol and mounting media
- Biodegradable, non-corrosive, non-flammable (Combustible)
- Contains no benzene and no toluene
- Low toxicity levels
- Minimal tissue shrinkage
- Reasonably fast drying and leaves no residue
In Research and Medical – Hematology, Cytology, Pathology, Microbiology, Histology, Pathology, Hospital
In Mechanical Applications – Cleaning of machine parts, cleaning tools, engines etc.
In Electronic Applications – Circuits boards, electronic components, tools.
General Applications – Clean metal and glass surfaces. Dilute with water can be used to clean all plastic surfaces. Removal of adhesives, glues, tars…
|Chemical name:||d-Limonene (Terpene Hydrocarbon, n.o.s.)|
|Composition:||d-Limonene, CAS [5989-27-5]98.0%
Butylated Hydroxyanisolve (BHA), CAS
|Flash Point (TCC):||121°F (49.4°C)|
|Boiling Point:||349°F (176°C) @ 760mmHg|
|Shipping:||Terpene Hydrocarbons, n.o.s. ID No.
04858003, Flammable Liquid, Group III
- W.E. Aldeen and D. Hale. "Use of Hem-De to eliminate toxic agents used for concentration and trichrome staining of intestinal parasites" J. of Clinical Microbiology, July 1992, 1893- 1895, Vol. 30, No. 7
- Metzger, Zvi DMD et al. "Gutta-Percha Softening: 'Hem-De' as a Xylene Substitute" J. of Endodontics". 26(7):385-388, July 2000.
- Devon C. Hale, MD; et al. "Use of a Single Slide Trichrome- Stained Concentrate for the detection of intestinal Parasites Stained Concentration Procedure for Ova and Parasites" American Journal of Clinical Pathology, Vol. 106, N0. 2. Aug. 1996.
- J. M. Miller et al. "Biodegradable, effective substitute for xylene in the Ehrlich indole procedure". J. of Clinical Microbiology, Aug. 1994, 2028-2030, Vol 32, No 8.
|RT||23412-01||Xylene Substitute||1 gal||110.00||Add to Cart|
|RT||23412-04||Xylene Substitute||4x1 gal||399.00||Add to Cart|
|RT||23412-05||Xylene Substitute||5 gal||450.00||Add to Cart|
*Hemo-De® is a product of Scientific Solvents.
Histo-Clear® (Distilled essential oils –food grade) and Histo-Clear II® (A mixtureof Aliphatic Hydrocarbon and Distilled essential oils – food grade, reduced citrus odor) are excellent non-toxic clearing agents replacing Xylene.
|RT||64110-01||Histo-Clear®||1 L||30.00||Add to Cart|
|RT||64110-04||Histo-Clear®||1 gal||80.00||Add to Cart|
|RT||64110-10||Histo-Clear®||5 gal||395.00||Add to Cart|
|RT||64111-01||Histo-Clear II®||1 L||19.00||Add to Cart|
|RT||64111-04||Histo-Clear II®||1 gal||64.00||Add to Cart|
|RT||64111-10||Histo-Clear II®||5 gal||295.00||Add to Cart|
Histosol™ is the original high flash point (114°F TCC) histological clearing agent. It is intended to be used as a replacement for xylene where the hazards associated with aromatic hydrocarbon vapors are to be reduced. Museum-quality tissue slides can be prepared with Hitosol without change in protocol or procedure. Histosol is manufactured from petrochemical products and is miscible in all proportions with ethanol, isopropanol, and t-butanol. It is also miscible with all paraffin-based tissue embedding media and all permanent mounting materials.
|RT||23405-01||Histosol – Xylene Substitute||1 gal||59.00||Add to Cart|
|RT||23405-04||Histosol – Xylene Substitute||4 x 1 gal||216.00||Add to Cart|
This unique primer affords you a better adhesion between your resin and specimen that you are embedding and prevents the sample from pulling away from the resin during sectioning.
|RT||50440-10||Z6040 Primer||100 ml||44.00||Add to Cart|