IHC Antigen Retrievers
EMS is proud to introduce a broad selection of Antigen Retrievers in IHC...
I. Trypsin Reagent
Description: Trypsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin; mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Trypsin digestion improves immunoreactivity of some antigens in FFPE tissue sections. For cytokeratin clone AE3 and AE1/AE3, this enzyme works much better than boiling the tissue with citrate buffer.Storage: 2-8°C. DO NOT FREEZE
Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: This enzyme is supplied as concentrated form along with buffer. The kit comes in 2 sizes. Small: Reagent B (Buffer) is 15 ml and the Reagent E (Enzyme) is 2 ml.
Large: Reagent B (Buffer) is 100 ml and the Reagent E (Enzyme) is 10 ml.
| Cat. # | Description | Qty. | Price | |
| 64142-01 | Trypsin Kit Small | kit | 75.00 | Add to Cart |
| 64142-02 | Trypsin Kit Large | kit | 145.00 | Add to Cart |
II. Pronase Reagent, Ready To Use
Description: Pronase is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pronase digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.Storage: 2-8°C. DO NOT FREEZE
Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: Ready-to-use Pronase reagent available in 2 sizes: 15 and 100 ml.
| Cat. # | Description | Qty. | Price | |
| 64142-03 | Pronase Reagent | 15 ml | 75.00 | Add to Cart |
| 64142-04 | Pronase Reagent | 100 ml | 145.00 | Add to Cart |
III. Pepsin Reagent, Ready To Use
Description: Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pepsin digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.Storage: 2-8°C. DO NOT FREEZE
Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: Ready-to-use Pepsin reagent available in 2 sizes: 15 and 100 ml.
| Cat. # | Description | Qty. | Price | |
| 64142-05 | Pepsin Reagent | 15 ml | 75.00 | Add to Cart |
| 64142-06 | Pepsin Reagent | 100 ml | 145.00 | Add to Cart |
IV. Citrate Buffer pH 6.0 (10x)
Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.Storage: 2-8°C. DO NOT FREEZE
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 6 antigen retriever solution.
Preparation of Working Solution: Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C.
| Cat. # | Description | Qty. | Price | |
| 64142-07 | Citrate Buffer pH 6.0 | 100 ml | 275.00 | Add to Cart |
| 64142-08 | Citrate Buffer pH 6.0 | 1000 ml | 125.00 | Add to Cart |
V. EDTA Buffer pH 8.5 (10x)
Description: This buffer is intended for heat-induced antigen retriever on
formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.Storage: 2-8°C. DO NOT FREEZE
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 8.5 antigen retriever solution.
Preparation of Working Solution: Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C.
| Cat. # | Description | Qty. | Price | |
| 64142-09 | EDTA Buffer pH 8.5 | 100 ml | 75.00 | Add to Cart |
| 64142-10 | EDTA Buffer pH 8.5 | 1000 ml | 125.00 | Add to Cart |
VI. Tris Buffer pH 10.0 (10x)
Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.Storage: 2-8°C. DO NOT FREEZE
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 10 antigen retriever solution.
Preparation of Working Solution: Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C.
| Cat. # | Description | Qty. | Price | |
| 64142-11 | Tris Buffer pH 10.0 | 100 ml | 75.00 | Add to Cart |
| 64142-12 | Tris Buffer pH 10.0 | 1000 ml | 125.00 | Add to Cart |
VII. ImmunoHistoZyme™, Ready-to-Use
Description: ImmunoHistoZyme is used for proteolytic digestion of
formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. ImmunoHistoZyme digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.Storage: 2-8°C. DO NOT FREEZE
Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: Ready-to-use ImmunoHistoZyme Reagent.
| Cat. # | Description | Qty. | Price | |
| 64142-13 | ImmunoHistoZyme | 15 ml | 75.00 | Add to Cart |
| 64142-14 | ImmunoHistoZyme | 100 ml | 145.00 | Add to Cart |
