The 2100 Retriever allows the use of up to 6 different buffers in one cycle, each of the Slide Chambers accommodating one buffer. Each individual Slide Chamber can accommodate up to 18 slides. You can optimize the recovery buffer (and/or test the usability of buffer for recovery) for 18 individual antibodies in one go.
Such runs allow the establishing of the best/acceptable epitope recovery conditions for all antibodies that can later be used in experiments, which is especially important if you plan an IF multicolor staining of tissue sections.
We advise to do the primary test for individual antibodies with immunoenzyme staining, as this allows for better analysis of the pattern of staining and (eventual) non-specific staining of some areas/structures in section (i.e. blood vessels, etc.).
For your convenience, we offer a complete set of different recovery buffers for the tests, containing 125 ml of each 10x buffer stock.