Description: Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pepsin digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: Ready-to-use Pepsin reagent available in 2 sizes: 15 and 100 mL.