For bacteria stain: Gram + / Gram – bacteria and filaments of nocardia, etc.
Stain results:
| Gram + Bacteria, Nocardia and Actinomyces Filaments: |
|
Blue |
| Gram – Bacteria, Nuclei: |
|
Red |
| Additional tissue elements: |
|
Yellow |
Note: Also see the Taylor Modification of the Brown and Brenn +/- technique noted for the varying differentiation available. Over-differentiation in the B&B sep 6 is a problem with some sections; run the control slides at varying rates to determine the amount for the specific organism.
References:
- Brown J.H. and Brenn, L. Bull. Johns Hopkins Hosp., 48:69 (1931)
- AFIP Manual of Histologic Staining Techniques: 3rd. ed., ed. G. Luna; New York: McGraw-Hill Publications, c. 1968, p. 222