NanoSuit® Aqueous Solution for SEM

Forms a thin, conductive, moisture barrier between specimens and a vacuum environment

Taking electron microscope images of biological objects in their natural state

What is the NanoSuit®

  • A novel technology which enables us to observe cells, microorganisms, etc. in a living state using scanning electron microscopy (SEM).
  • NanoSuit forms a very thin barrier layer on the surface of an object and the barrier layer holds moisture in the object under vacuum condition in electron microscopy. Also, the barrier layer is electrically conductive.
  • Therefore, NanoSuit makes it possible to observe biological objects with their natural image texture.
  • NanoSuit is very easy to use. Only dropping the NanoSuit solution onto the objects, then you can observe the objects using SEM. (You don't need any other fixation procedures.)

History

Inventor Professor Takahiko Hariyama tried to observe various microorganisms using SEM, but almost all organisms died due to losing moisture under the vacuum condition in the electron microscopy.

However, he discovered a kind of fly larva was able to keep alive under the vacuum condition.

Prof. Hariyama investigated that phenomenon and revealed that a very thin barrier layer was formed on the surface of the larva by electron beam irradiation. The barrier layer held moisture and kept the larva alive.

Hariyama and his colleagues had tried to mimic this phenomenon artificially and invented "NanoSuit" technology.

Science

The "NanoSuit"

  • Aqueous solution of the biocompatible polymer. (biologically safe material)
  • Just drop onto the objects.
  • Polymerize by electron beam or plasma irradiation, then a thin barrier layer is formed on the surface of the objects.
  • The barrier layer holds moisture contained in the objects and its conductivity provides clear SEM images.

References

Kawasaki, H., Itoh, T., Takaku, Y. et al. The NanoSuit method: a novel histological approach for examining paraffin sections in a nondestructive manner by correlative light and electron microscopy. Lab Invest 100, 161-173 (2020). https://doi.org/10.1038/s41374-019-0309-7

Shinmura K, Kawasaki H, Baba S, et al. Utility of Scanning Electron Microscopy Elemental Analysis Using the 'NanoSuit' Correlative Light and Electron Microscopy Method in the Diagnosis of Lanthanum Phosphate Deposition in the Esophagogastroduodenal Mucosa. Diagnostics (Basel). 2019;10(1):1. Published 2019 Dec 18. doi:10.3390/diagnostics10010001

Takaku Yasuharu, Suzuki Hiroshi, Kawasaki Hideya, Ohta Isao, Ishii Daisuke, Hirakawa Satoshi, Tsutsui Takami, Matsumoto Haruko, Takehara Sayuri, Nakane Chinatsu, Sakaida Kana, Suzuki Chiaki, Muranaka Yoshinori, Kikuchi Hirotoshi, Konno Hiroyuki, Shimomura Masatsugu and Hariyama Takahiko 2017A modified 'NanoSuit®' preserves wet samples in high vacuum: direct observations on cells and tissues in field-emission scanning electron microscopyR. Soc. open sci.4160887.

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NanoSuit Solution I

Solution used with the NanoSuit technology that allows you to observe cells, microorganisms, etc. in a living state using SEM (scanning electron microscopy).
Cat # Description MSDS Pack Price Quote Quantity
Cat #: 11111-01 Description: NanoSuit Solution I Pack: 5 mL Price: $1,320.00 Add to Quote:
Cat #: 11111-03 Description: NanoSuit Solution III Pack: 5 mL Price: $1,320.00 Add to Quote: