NanoSuit® is an innovative aqueous solution for SEM that enables researchers to observe cells, microorganisms, and other similar materials that may otherwise deform or deteriorate in the high-vacuum environment of traditional SEMs. This novel technology forms a very thin layer of hydrophobic material on the surface of an object, which holds moisture in the object and is electrically conductive. In doing so, the NanoSuit aqueous solution makes it possible to analyze biological objects and other liquid samples with their natural image texture.
How to Use NanoSuit Aqueous Solution for SEM
Extremely easy to use, the NanoSuit solution is simply dropped onto an object or sample, which is then ready for observation. Samples coated with the NanoSuit aqueous solution don’t require any other ﬁxation procedures or dehydration, expediting the preparation process. For a more detailed look at sample preparation and observation using the NanoSuit aqueous solution, check out the following video.
History of NanoSuit Aqueous Solution
The idea of a NanoSuit aqueous solution was first proposed by inventor Professor Takahiko Hariyama and colleagues in 2013. At the time, the professor was attempting to observe various microorganisms using SEM but quickly encountered a troubling recurrence – nearly all of the microorganisms died during observation due to losing moisture under the vacuum condition within the electron microscope.
During this process, however, he also discovered a type of fly larva that was able to stay alive under the vacuum condition, which led him to further investigation. In doing so, Professor Hiriyama determined that a very thin, natural aqueous solution barrier layer was formed on the surface of the larva through electron beam irradiation. This barrier layer retained moisture and kept the larva alive during the process.Hariyama and his colleagues then tried to mimic this phenomenon artificially, inventing "NanoSuit" technology: an aqueous solution of biocompatible polymer that becomes polymerized by electron beam or plasma irradiation to create a thin, moisture-protecting barrier on the surface of samples. Now, researchers can utilize these SEM solutions to create high-quality, accurate imaging.
Kawasaki, H., Itoh, T., Takaku, Y. et al. The NanoSuit method: a novel histological approach for examining paraffin sections in a nondestructive manner by correlative light and electron microscopy. Lab Invest 100, 161-173 (2020). https://doi.org/10.1038/s41374-019-0309-7
Shinmura K, Kawasaki H, Baba S, et al. Utility of Scanning Electron Microscopy Elemental Analysis Using the 'NanoSuit' Correlative Light and Electron Microscopy Method in the Diagnosis of Lanthanum Phosphate Deposition in the Esophagogastroduodenal Mucosa. Diagnostics (Basel). 2019;10(1):1. Published 2019 Dec 18. doi:10.3390/diagnostics10010001
Takaku Yasuharu, Suzuki Hiroshi, Kawasaki Hideya, Ohta Isao, Ishii Daisuke, Hirakawa Satoshi, Tsutsui Takami, Matsumoto Haruko, Takehara Sayuri, Nakane Chinatsu, Sakaida Kana, Suzuki Chiaki, Muranaka Yoshinori, Kikuchi Hirotoshi, Konno Hiroyuki, Shimomura Masatsugu and Hariyama Takahiko 2017A modified 'NanoSuit®' preserves wet samples in high vacuum: direct observations on cells and tissues in field-emission scanning electron microscopyR. Soc. open sci.4160887.
|Cat #: 11111-01||Description: NanoSuit Solution I||MSDS||Pack: 5 mL||Price: $1,320.00||Add to Quote:||
|Cat #: 11111-02||Description: NanoSuit Solution II||Pack: 5 ml||Price: $1,320.00||Add to Quote:||
|Cat #: 11111-03||Description: NanoSuit Solution III||MSDS||Pack: 5 mL||Price: $1,320.00||Add to Quote:||