Forms a thin, conductive, moisture barrier between specimens and a vacuum environment
Inventor Professor Takahiko Hariyama tried to observe various microorganisms using SEM, but almost all organisms died due to losing moisture under the vacuum condition in the electron microscopy.
However, he discovered a kind of fly larva was able to keep alive under the vacuum condition.
Prof. Hariyama investigated that phenomenon and revealed that a very thin barrier layer was formed on the surface of the larva by electron beam irradiation. The barrier layer held moisture and kept the larva alive.
Hariyama and his colleagues had tried to mimic this phenomenon artificially and invented "NanoSuit" technology.
Kawasaki, H., Itoh, T., Takaku, Y. et al. The NanoSuit method: a novel histological approach for examining paraffin sections in a nondestructive manner by correlative light and electron microscopy. Lab Invest 100, 161-173 (2020). https://doi.org/10.1038/s41374-019-0309-7
Shinmura K, Kawasaki H, Baba S, et al. Utility of Scanning Electron Microscopy Elemental Analysis Using the 'NanoSuit' Correlative Light and Electron Microscopy Method in the Diagnosis of Lanthanum Phosphate Deposition in the Esophagogastroduodenal Mucosa. Diagnostics (Basel). 2019;10(1):1. Published 2019 Dec 18. doi:10.3390/diagnostics10010001
Takaku Yasuharu, Suzuki Hiroshi, Kawasaki Hideya, Ohta Isao, Ishii Daisuke, Hirakawa Satoshi, Tsutsui Takami, Matsumoto Haruko, Takehara Sayuri, Nakane Chinatsu, Sakaida Kana, Suzuki Chiaki, Muranaka Yoshinori, Kikuchi Hirotoshi, Konno Hiroyuki, Shimomura Masatsugu and Hariyama Takahiko 2017A modified 'NanoSuit®' preserves wet samples in high vacuum: direct observations on cells and tissues in field-emission scanning electron microscopyR. Soc. open sci.4160887.