The signal-to-noise ratio determines the quality of any detection experiment. This ratio is in principle determined by the characteristics of specimen and detection reagents. With adequate blocking and incubation media, background reactions are minimized whereas specific reactions are not hampered.
Aurion has developed Blocking Solutions which effectively block ‘sticky’ surfaces by multi-point hydrophobic and charge-based interactions. The Blocking Solutions are specifically designed to meet the characteristics of each type of secondary conjugate.
The AURION Blocking Solutions are used to prevent immunoreagents from binding non-specifically to specimens with “sticky” surface properties.
Procedures to eliminate background comprise three main steps are:
- To suppress residual aldehyde activity,
- To saturate multipoint hydrophobic moieties and positive charges with high molecular weight compounds such as those present in the AURION Blocking Solutions,
- To reduce aspecific binding of immunoreagents caused by hydrophilic interaction with competing molecules in the incubation and washing solution. AURION BSA-c™ is a particularly effective reagent for this purpose.
These steps should be balanced for optimum results.
AURION Blocking Solutions are prepared using specially selected compounds. All ruminant proteins are obtained from healthy livestock.
AURION Blocking solutions contain Bovine Serum Albumin and Cold Water Fish Skin Gelatine in phosphate buffered saline with sodium azide as preservative. Normal serum may have been added as indicated on the label. The blocking capacity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods 74, (1984), 353.
Each package contains 30 ml of solution. It accommodates 300 specimens for light microscopy at 100 µl/specimen (~ 3 drops), or 1000 EM grids at 30 µl/specimen (~ 1 drop).