Successful immuno-detection experiments depend upon the use of high-quality products that, used in combination, yield optimum results. Aurion reagent kits are specifically designed for this purpose.
Now you also have this option for double labeling experiments with this new series of kits containing different combinations of secondary antibody gold reagents commonly used in double labeling experiments. With the addition of Aurion blocking reagent and Aurion BSA-c (10%) incubation buffer additive, these kits have all you need to achieve the best signal-to-noise ratio possible.
Conventional Immunogold Double Labeling Kits contain:
- 1 x 10ml 6nm ImmunoGold Reagent
- 1 x 1.4ml 15nm ImmunoGold Reagent
- 30ml Blocking Solution
- 30ml BSA-c
Ordering Aurion products in kit form will give you a cost-savings on the total price of the individual components.
For ease of discrimination between the two antigenic sites, the combination of 6nm and 15nm gold nanoparticles has been depicted. A recent example of the combined use of Aurion 6nm and 15 nm gold conjugates can be found in: Munoz O., et al. (2022) Active PD-L1 incorporation within HIV virions functionally impairs T follicular helper cells. PLOS Pathogens 18(7); e1010673.