Cryo 35° Cryo 45°

Available for wet or dry sectioning with a 25°, 35° or a 45° angle.
Section thickness range 30-300 nm. Cutting edge sizes 1.5-4 mm.

DiATOME cryo diamond knives may be used for all low temperature situations such as sectioning of cryo-protected specimens, frozen hydrated specimens and industrial samples such as polymers and rubber.

There are numerous advantages to our cryo diamond knives as opposed to glass knives:

• They produce thinner sections.
• Ultrathin and semithin sections with the same knife.
• Easy to produce long ribbons of sections.
• Less compression and greater uniformity of sections.
• You gain time. No knife manufacturing, no knife changing, hence no waiting for temperature equilibration.

Cryo 25°

The Cryo 25° knife is designed for sectioning frozen hydrated specimens. The 25° angle results in the least possible compression and the best structure preservation.

Cryo 35° Cryo 45°

The 45° knife is well suited for routine cryo sectioning.

The 35° knife allows you to section with considerable reduction of mechanical damage to the specimen (up to 20% less compression as compared to the 45° knife) and, in turn, better structural preservation of most frozen samples (Ref 6, 7, 8).

The triangular holder for dry sectioning and the boat for the sectioning with a liquid (DMSO/water and others) are made of a special copper/nickel alloy. They both guarantee excellent cold or heat transmission.

The seal is made of a cold-resistant epoxy resin.

Styrene-butadiene block copolymer x 25'000, Ronald Walter, BASF Aktiengesellschaft, Polymer Physics, D-67056 Ludwigshafen.