The CEMOVIS 25° and 35° knife is designed for sectioning frozen hydrated specimens. The 25° angle results in the least possible compression and the best structure preservation (H.M. Han et al., Journal of Microscopy, Vol. 230, Pt. 2, pp. 167 – 171, 2007).
Please note: best results are achieved at low humidity, when the cryo-ultramicrotome is placed in a glovebox and the sections attached by electrostatic force (J. Pierson et al., Journal of Structural Biology 169, pp. 219 – 225, 2010).