Hirano-Zimmerman Method for Nerve Cells and Fibers (1962)

For the staining of nerofibrils, dendrites, axis cylinders, senile plaques, etc.

Stain results:

Nucleolus and nuclear membrane:   Black
Neurofibrils, dendrites and axis cylinders:   Black
Cytoplasm of astrocytes and the cytoplasmic
membranes of macrophages:
  Gray
Senile plaques:   Black

References:

  • AFIP Manual of Histological Staining Methods, 3rd ed., Ed. L.Luna: New York: McGraw-Hill Pub., c 1968, p. 198
  • Hirano, A. and Zimmerman, H.M., Arch. Neurol., 6:114-122, 1962
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