UranyLess has a strong contrasting power, however, we recommend a Lead Citrate counterstain to enhance the contrast. You can follow the protocol of the UranyLess / Lead Citrate double contrast by watching the video. EMS recommends Lead Citrate 3%, ready to use. The special pump delivers the product without letting in air, thus preserving the solution and preventing CO2 dissolution. It is convenient to use because it performs in any position, even upside down. Comes in either a 30 ml Airless bottle or a 30 ml Airless Syringe. The Syringe is meant to be used exclusively with the RMC TEM Stainer QG3100
References:
"Easier and Safer Biological Staining: High Contrast UranyLess Staining of TEM Grids using mPrep/g Capsules." Benmeradi N1,2, Payre B2 and Goodman SL3.
- Delta Microscopies, 22 bis, route de Saint Ybars, 31190 Mauressac, France
- Université Toulouse, CMEAB Faculté Medecine, 118 route Narbonne, 31062, Toulouse, France
- Microscopy Innovations LLC, 213 Air Park Rd, Suite 101, Marshfield, WI, 54449, USA
"C-Nap1 mutation affects centriole cohesion and is associated with a Seckel-like syndrome in cattle." Nature Communications. Published 23 Apr 2015. Sandrine Floriot, all.
* in airless bottle