NIGHTSEA SFA Fluorescence Adapter for Hirox HR-1020E Lens

Overview

The NIGHTSEA Model SFA Fluorescence Adapter system can add a versatile fluorescence imaging capability to the Hirox digital microscope. Visit our website for a full gallery of images made with the NIGHTSEA adapter and the Hirox microscope.

Our adaptations to the Hirox system are concerned with the lens. We now offer variants of our fluorescence adapter system that work with the HR-2500E and HR-1020E lenses. (We may be able to support older lenses – contact NIGHTSEA for more information.)

Fluorescence solution

The key elements of any fluorescence system are:

• A light source that produces sufficient energy in the appropriate 
  wavelength range to excite fluorescence in the sample of interest 
• A barrier filter in the viewing path that blocks reflected excitation light 
  while transmitting the fluorescence emitted by the sample

NIGHTSEA implements these for the Hirox system with:

• high intensity LED light sources available in five excitation wavelength 
  ranges (see list on next page) 
• emission barrier filters that can be easily added to the Hirox lenses
  • The filters for the HR-2500E and HR-1020E lenses simply insert into the Analyzer slot.

Comprehensive Solution Kit

The components of a NIGHTSEA system that adds a single excitation/emission wavelength combination on a Hirox microscope are:

• Flexible gooseneck lamp base with LED drive circuit and intensity control
• Universal power supply (120/240VAC, 50/60Hz) with international plug set
• Excitation light source
• Emission barrier filter
• Barrier filter glasses for fluorescence viewing and eye safety
• Padded carrying case

Additional wavelength sets are implemented by adding a matched set (light plus filter set) consisting of a light source, barrier filter, and filter glasses.

We are currently working on adapters for the full range of Hirox lenses, for use with all of our wavelength options. Please contact Hirox or NIGHTSEA to discuss your application and compatible solutions.

Wavelength Sets

There are five excitation/emission wavelength combinations available for the system.