Many compounds have been added empirically to immunolabeling solutions for the purpose of minimizing background staining. Based on years of experience and controlled testing, Aurion has selected a group of compounds that are proven to be the most effective in their background reduction action. Researchers can obtain these items separately from Aurion and prepare blocking and incubation media to suit the needs of their own protocols.
The ready-to-use Blocking Solutions and the incubation media additive BSA-c™ are tuned for optimum background prevention and signal-to-noise ratio. In-depth information can be found in the respective product data sheets.
AURION also offers a number of components that allow researchers to formulate blocking, incubation and wash solutions according to the needs of their own experiments.
Aurion's Bovine Serum Albumin is obtained from healthy livestock. BSA should be dissolved in an appropriate buffer, such as phosphate buffered saline, taking care not to denature the protein by foaming. The addition of BSA may cause a drop in pH of the final solution and correction may be required. As a preservative the use of NaN3 or Kathon CG is recommended.
Normal sera are used to counteract the non-specific interaction between the sample and immunoglobulins. They can be added to the blocking solution and the incubation media.
As a rule the normal serum species should be the same as the secondary antibody species (e.g. use normal goat serum with goat-anti-rabbit conjugates).
Note: normal sera should not be used in combination with Protein A and Protein G gold reagents.
The products should be stored at 4-8°C.
Freezing is not recommended