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- TEM Grids
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UranyLess Applications
All photos taken with TEM.
Reconstituted Epidermis
Preparation of the sample using the following protocol:
- Fixing Classic Glutaraldehyde, Osmium, Epon / Araldite
- Cutting Ultra-Thin, Double UranyLess Contrast and Lead Citrate
Mouse Muscle, Nerve
Preparation of the sample using the following protocol:
- Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
- Ultrafine Cup - UranyLess Contrast 1 minute followed lead Citrate 1 minute
Mouse Ovarian Follicle
Preparation of the sample using the following protocol:
- Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
- Ultrafine Cup - Contrast UranyLess Lead -Citrate
Mouse Kidney
Preparation of the sample using the following protocol:
- Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
- Ultrafine Cup - UranyLess Contrast 1 minute followed lead Citrate 1 minute
Mouse Cardiac Muscle
Preparation of the sample using the following protocol:
- Classic - Fixing Glutaraldehyde, Osmium, Epon Ultrafine
- Cups, Double UranyLess Contrast and Lead Citrate
Mouse Cardiac Muscle
Preparation of the sample using the following protocol:
- Classic - Fixing Glutaraldehyde, Osmium, Epon Ultrafine
- Cups, Double UranyLess Contrast and Lead Citrate
Plant Tissue
Preparation of the sample using the following protocol:
- Glutaraldehyde Fixation Classic - Osmium - Included in Epon
- Contrast the UranyLess monitoring Lead Citrate
Drosophila Larva
Preparation of the sample using the following protocol:
- Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
- Ultrafine Cup - Contrast UranyLess lead -citrate
Phage T6
Preparation of the sample using the following protocol:
- Staggering Phage T6 on a G300-Cu grid Covered with a Carbon Formvar Film. Ionization 1 minute
Cross-Sectional Bacteria
Preparation of the sample using the following protocol:
- Fixing Classic Glutaraldehyde, Osmium, EPON
- Cutting Ultrafine, Double Contrast UranyLess and Lead Citrate.
Bacteria E. Coli
Negative Staining for 2 Minutes UranyLess Bacteria Like E. Coli (Adherent and Invasive (ACSI) LF82) Which Have Pili and Flagella.
Sacculina Crustaceans (Small Parasitic Crustacean)
Preparation of the sample using the following protocol:
- Classic Glutaraldehyde Fixation, Osmium, Epoxy Inclusion
- Fine Cups - Contrast to the Aqueous UranyLess to 60°C on a Hotplate without Lead Citrate Post Coloring
Intestine
Preparation of the sample using the following protocol:
- Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
- Ultrafine Cup - Contrast UranyLess Lead -Citrate
Mouse Liver
Preparation of the sample using the following protocol:
- Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
- Ultrafine Cup - Contrast UranyLess Lead - Citrate
Adrenal Gland Gerbil Sahara
Preparation of the sample using the following protocol:
- Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
- Ultrafine Cup - Contrast UranyLess lead -citrate
Parsley and Rosebush
Preparation of the sample using the following protocol:
- Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
- Ultrafine Cup - Contrast UranyLess lead -citrate
Culture Cells
Preparation of the sample using the following protocol:
- Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
- Ultrafine Cup - Contrast UranyLess lead -citrate
PLC Contrast Leica EM Stain
Preparation of the sample using the following protocol:
- Classic-Glutaraldehyde Fixation PFA, Osmium, Epon
- Ultrafine Cup - contrast Uranyless Lead -Citrate
Chantal Cazevieille CRIC / IURC INSERM Montpellier tested aqueous UranyLess in the Leica brand grid contrast controller on different tissues, Drosophila heart atrium, retina, cochlea and ileum (Gut). The tissues were fixed according to the standard protocol 2.5% Glutaraldehyde in PHEM buffer, the post fixation in 0.5% osmium in 0.8% potassium ferrocyanide in RT for 2 hours. The sections are collected on single-hole or 200 mesh grids.
The treatment of the grids is UranyLess 7mn lead citrate followed 7 minutes.
We present here some images made by Hitachi transmission electron microscope with a digital camera AMT.
You will notice that the combined action of potassium ferrocyanide and UranyLess reveal a marked way the cyto-membranes in the ileum.
Yeasts
Preparation of the sample using the following protocol:
- Classic Fixation Glutaraldehyde - Osmium - Included in Epon
- Contrast the UranyLess monitoring Lead Citrate
Trematodes
Preparation of the sample using the following protocol:
- Classic Glutaraldehyde Fixation, Osmium, Inclusion in Spurr Resin
- Contrast the UranyLess monitoring Lead Citrate
