16-Well Glass Slide

The growth of various cell types on plastic, soda lime glass and borosilicate coverglass were examined. The surfaces were either unmodified, coated with polylysine, or stably surface modified with non-biological reagents as described by Kleinfield, et al. (1988). All substrates were assembled into Lab-Tek® and Lab-Tek®II Chamber Slide™ products.

Product Features Lab-Tek® Lab-Tek® II Lab-Tek® II CC2
Configurations 1, 2, 4, 8, 16 well, flask style 1, 2, 4, 8 well 1, 2, 4, 8 well
Media chamber material Proprietary cell culture grade polystyrene (PS) Proprietary cell culture grade polystyrene (PS) Proprietary cell culture grade polystyrene (PS)
Slide Materials Soda lime glass Permanox® plastic Polystyrene plastic
Soda lime glass Soda lime glass
Cover Clear polystyrene, no handle, facilitates microscopic viewing Clear polystyrene, with handle for aseptic manipulations Clear polystyrene, with handle for aseptic manipulations
Sealant Non-toxic medical grade silicone
• Remains on slide after detachment
Biocompatible acrylic adhesive
• Remains with media chamber after detachment
Biocompatible acrylic adhesive
• Remains with media chamber after detachment
Reagent ‘isolator’ Created by removable
silicone gasket
Created by hydrophobic border Created by hydrophobic border

Chamber separation
No separation tool required Requires separation tools Requires separation tools
Growth Surface For adherent cell culture Glass slides
• 6-stage high purity water wash
Permanox® plastic
• Cell culture treated
Polystyrene
• Cell culture treated
For adherent cell culture RS treated glass slide
Proprietary wash
For adherent cell culture chemically modified surface that’s optimal for fastidious cells
Mimics a poly-lysine coated.
Biological testing BHK-21, Hep-2 cells BHK-21, Hep-2 cells Primary chick brain neurons, Hep-2 cells
Leak tested Yes Yes Yes
Slide background fluorescence Glass: Minimal fluorescence Permanox®: Minimal fluorescence Polystyrene: Autofluorescence at <485nm Glass:
• No endogenous fluorescence
Glass:
• No endogenous fluorescence
Coverslip compatibility Yes, following gasket removal Yes Yes
Writing surface “Sand-blasted” frosted end Super Frost® white printed end SuperFrost® blue printed end

Lab-Tek® Chamber Slide Specifications: Well Visible Working

Growth Surface Well Size (cm2) Visible Area (cm2) Working Volume (ml)
1 Chamber, Glass/Permanox 9.4 9.4 2.5-4.5
2 Chamber, Glass/Permanox 4.2 4.2 1.2-2.0
4 Chamber, Glass/Permanox 1.8 1.8 0.5-0.9
8 Chamber, Glass/Permanox 0.8 0.8 0.2-0.4
16 Chamber, Glass 0.4 0.4 0.1-0.2
Flaskette, Glass 10.0 10.0 2.5-5.0

Lab-Tek® II Chamber Slide Specifications

Growth Surface Well Size (cm2) Visible Area (cm2) Working Volume (ml)
1 Chamber, Glass, CC2 10.0 8.6 4.0-5.0
2 Chamber, Glass, CC2 4.2 4.0 2.0-2.5
4 Chamber, Glass, CC2 2.4 1.7 1.0-1.3
8 Chamber, Glass, CC2 1.2 0.7 0.5-0.6

Lab-Tek® Chamber Slide™ System

  • Developed for microscopy of in situ cell culture
  • Cells grow on a standard microscope slide
  • Media chamber can be removed when culturingis complete
  • Useful for viral and mycoplasma testing, chromosome studies toxicity tests and immunocytology
  • Permanox® plastic and glass do not interfere with fluorescent labels

Permanox® is a special molding resin, which meets the growing requirements of most anchorage dependent cells; also excellent chemical resistance allows for cell fixation and the staining of organic solvents for EM and other techniques. Low oxygen permeation makes Permanox® ideal for anoxic cell growth.

Chamber slides combine a media-containing chambered super-structure with a standard soda lime glass slide or Permanox® plastic slide, sealing the two together with a biologically inert silicone gasket. After cells are grown, the chamber and gasket are easily removed. The slide may then be fixed, stained, cover slipped, and filed with the usual convenience. These slides, once fixed and stained, can be embedded using our Slide Duplicating Mold (Cat. 70170).

Lab-Tek® Chamber Slide™ System all come packed in a sterile sealed tray, 8 units per tray, 2 trays per pack and 12 trays per case.

arrow12Lab-Tek® Glass Chamber Slide™

Soda lime glass slide does not interfere with fluorescent label.

SKU: 70422
Pack: 12 Tray
$1,298.50
Products specifications
Attribute name Attribute value
Material Glass