Stockert,J.C. (1977). Sodium Tungstate as a stain in electron microscopy. Biol. Cellul., 29:211. Takeuchi, I.K. (1981). Differential staining of nucleoli and chromatin by sodium tungstate. J. Electron Microsc., 30:15.
Similar to Osmium tetroxide in use as fixation/staining agent for EM. Offers excellent staining of saturated & unsaturated polymer materials. Stabilizies thin sections under em.
Very low viscosity monomeric styrene penetrates rapidly into tissues. Soluble in ethanol and acetone. Infiltration and embedding can be done by styrene alone. Polymerize by UV or heat within 2-3 days without benzoyl peroxide.
Sym-Collidine is used in combination with osmium tetroxide to provide excellent fixation and stable buffering. pH does not change when distilled water or osmium tetroxide solution is added.
Thermoplastic adhesive for mounting powders and small particles for SEM. Does not contain solvents and is stable in high vacuum. It is not sticky at room temperature but becomes adhesive at 40°C and melts at 120°C.
Organo-silicon compound, tetramethylsilane (TMS) is an ideal solvent for tissue drying for SEM. It is chemically inert, has a bp of 26.6°C, and is soluble in organic solvents but insoluble in water. These properties result in excellent tissue detail.