Bismuth specifically stains Golgi beads and interchromatin granules after fixation with glutaraldehyde, whereas it stains nucleoli, basic protein, and biogenic amines after formaldehyde fixation. Bismuth staining of sections is relatively nonspecific: it can stain glycogen, lysosomes, ribosomes, polysaccharides, ferritin, and DNA. M.A. Hayat, "Basic Techniques for TEM" p. 184 (1986).
1. Specific En Bloc Staining (Locke & Huie, 1977)
Enhancing Contrast in General (Riva, 1974). Kit contains 5x10 ml Solution A(Sodium tartrate in NaOH) 5x200mg Ingredient B (Bismuth subnitrate).