Many compounds have been added empirically to immunolabeling solutions for the purpose of minimizing background staining. Based on years of experience and controlled testing, Aurion has selected a group of compounds that are proven to be the most effective in their background reduction action. Researchers can obtain these items separately from Aurion and prepare blocking and incubation media to suit the needs of their own protocols.
The ready-to-use Blocking Solutions and the incubation media additive BSA-c™ are tuned for optimum background prevention and signal-to-noise ratio. In-depth information can be found in the respective product data sheets.
AURION also offers a number of components that allow researchers to formulate blocking, incubation and wash solutions according to the needs of their own experiments.
Tween-20™ is a non-ionic detergent with a molecular weight of about 600 and a critical micelle concentration (CMC) of 0.06-0.07% in water at room temperature. Its working mechanism may in part be based on its action as a detergent, binding to the hydrophobic moieties of water insoluble compounds, rendering them hydrophilic. In addition, immuno-compounds may become incorporated into micelles when the Tween-20™ concentration is higher than the CMC, for instance at 0.1 % in PBS at pH 7.4.
The products should be stored at 4-8°C.
Freezing is not recommended.