Brown and Brenn Method for Gram Positive and Gram Negative Bacteria

For bacteria stain: Gram + / Gram – bacteria and filaments of nocardia, etc.

Stain results:

Gram + Bacteria, Nocardia and Actinomyces Filaments:   Blue
Gram – Bacteria, Nuclei:   Red
Additional tissue elements:   Yellow

Note: Also see the Taylor Modification of the Brown and Brenn +/- technique noted for the varying differentiation available. Over-differentiation in the B&B sep 6 is a problem with some sections; run the control slides at varying rates to determine the amount for the specific organism.

References:

  • Brown J.H. and Brenn, L. Bull. Johns Hopkins Hosp., 48:69 (1931)
  • AFIP Manual of Histologic Staining Techniques: 3rd. ed., ed. G. Luna; New York: McGraw-Hill Publications, c. 1968, p. 222
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