Ready to Use:
UranyLess in 30% Ethanol is a reagent specifically developed for tissues that are difficult to contrast, such as very dense, fibrous, or lipid-rich tissues (e.g., cornea). The alcohol acts as a vector, allowing UranyLess to penetrate deep into these tissues and improve contrast.
It serves as an alternative to traditional uranyl acetate for biological sample preparation, providing high-resolution imaging without the use of hazardous materials.
Its ethanol-based formulation enhances contrast penetration while preserving the integrity of lipid structures.
Key Features:
- Prepared in a 30% ethanol solution in water.
- Ethanol enhances contrast penetration, particularly in collagen-rich tissues.
- The 30% ethanol concentration provides an optimal balance for deep tissue penetration while preserving lipid structures.
Sample Preparation Protocol with UranyLess in 30% Ethanol
- Contrast the Sample:
- Apply UranyLess in ethanol to the sample for 1 to 2 minutes.
- Wash the Sample:
- Rinse the sample with a drop of 30% alcohol for 1 minute.
- (Optional) Contrast Enhancement:
- To further enhance contrast, apply lead citrate (#22410) for 1 minute.
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Transverse and longitudinal sections of corneal fiber bundles.
Copyright Dr. Chantal Cazveille-INM Montpellier, France
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