Controlled Tissue Bath

The 7000smz-2 unit with a Z-axis deflection of Sub-Micron has a blade advance controllable to 10 microns/second. The 5100mz-Plus and 5100mz are very competitively priced high precision units with a Z-axis deflection of only 1-2 microns and a blade advance controllable to 10 microns/sec. On all units the section thickness step size is 0.001 mm. The 7000smz-2 and 5100mz-Plus are supplied with their own Z-axis calibration verifier.

All types of sectioning are possible including sectioning for visual patching of neurological tissue, heart, and lung, and much more...

The effect of excessive Z-axis deflection on the health and viability of the tissue preparation has been much discussed since the publication of Jonas et al. (2003) and the 7000smz-2 will now deliver perfect sections every time with sub-micron Z-axis deflection across a wide range of vibration speeds and amplitudes. The affordable 5100mz-Plus offers almost the same features with Z-axis deflection of 1-2 microns at all vibration speeds and amplitudes, which is less than most other microtomes on the market.

The all-new user interface is both easy to use and versatile. The 7000smz-2 and both 5100mz models offer similar operation at the press of a button. The versatility includes simple operation at the push of a button or a range of changeable and programmable parameters and a menu enables the settings of your own preferences.

The 7000smz-2 also gives you longevity of performance; in other words it not only gives submicron performance out-of-the-box, but the advance vibrating mechanism does not contain bearings and other components subject to wear. Consequently it will retain the sub-micron Z-axis deflection performance for years to come, giving you consistency in your biological preparation.

Tissue cooling is accomplished by the use of either an ice-water bath or an electronically controlled thermo-electric cooler. Other options include a LED cold light source and a magnifier or a stereoscope for clear observation whilst slicing.

The new 5100mz series Vibrating Microtomes share the user interface with the premium 7000smz-2, placing a wide range of adjustable parameters at the user's fingertips. Use in fully manual or the semi-automated "slice window" mode which automatically remembers the start and finish position of the slice.

Features at a Glance

7000smz-2:

  • Includes a Z-axis calibration unit
  • Minimal Z-axis deflection (less than 1µm) at all speeds and amplitudes
  • Z-axis blade adjust minimizer
  • Blade holder angle to user requirement
  • Set start and stop position of blade travel
  • Tissue sample automatically retracted before blade returns to start point
  • Full range of adjustable parameters with 8 customizable user profiles
  • Vibration speeds from 50 to 120Hz
  • Amplitudes from 0.5 mm to 2.5 mm
  • Controlled blade advance at 10 microns per sec
  • "Auto" programming by storage of the first slicing
  • Ice water bath easily removed for cleaning
  • Optional LED light and scope for clear observation
  • Leaf spring vibratory mechanism for optimal longevity and accuracy
Specifications 7000smz-2
Section thickness step size 1µm
Vertical (Z-Axis) calibration device
'Opti-cal' is calibrated with metrology equipment traceable to National Standards
'Opti-cal' included as standard
Optimal Z-axis Deflection Sub-µm (± 0.1 µm)
Max specimen size 33 x 50 x 19
Maximum (vertical) travel of bath table 19 mm
Memory to store section thickness Yes
Multiple user settings 8 different
Cutting head advance speed while slicing From a minimum of -1.00 mm/sec, through zero to a maximum of +1.00 mm/sec
Cutting head advance speed resolution while slicing 0.01 mm/sec
Cutting head advance speed max (fast mode when not slicing) +/- 4.0 mm/sec
Specimen retraction as blade retracts Yes
Cutting head retraction speed (fast mode) 4.0 mm/sec
Operating Modes Manual, semi-auto 'slice window' or fully automated 'profile repeat'
Blade oscillation frequency range (dependent on amplitude) 50-120 Hz
Frequency step size 5 Hz
Blade oscillation amplitude Minimum: 0.5 mm (nominal)
Maximum: 2.5 mm (nominal)
Amplitude step size 0.25 mm (nominal)
Cooling options Ice bath or optional Model 7610A Tissue Bath Cooler
Power requirements 115VAC 60 Hz or 230VAC 50 Hz
(selectable)
Power rating 100W
Fuse rating (115v or 230v) T2A 250VAC
Light source 100-240VAC 3W mains connection
Dimensions 420 mm W x 400 mm D x 270 mm H (excluding light source and microscope)
Weight 35 kg
Packaged weight & dimensions Wooden crate 70 x 54 x 48 cm, 55 kg

 

Options

1. Temperature Controlled Standard Tissue Bath

Product Description

The 7610-A Tissue Bath Cooler Unit is intended for use with our 7000 and 5100 range of vibrating microtomes. Unfixed brain slices sectioned at 4°C give better tissue preservation and are viable for longer in-vitro recordings. Additionally, some enzyme histochemical techniques give better staining results when sectioned at low temperatures.

The 7610 Series coolers use "Peltier" thermoelectric elements. The stainless steel tissue bath and mount are detachable to allow sterilization by autoclave if required.

The equipment comprises of a mains operated control unit, cooling element, tissue bath and specimen holder. The control unit houses a power supply and temperature control circuitry. The cooling element assembly consists of thermoelectric "Peltier" elements, temperature feedback sensors and a cold water fed heat exchanger.

Current from the power supply flows through the thermoelectric elements, which act as heat transfer units. Heat is drawn off, cooling the solution in the tissue bath. The heat generated by this process is removed by the water supply fed through the heat exchanger. The unit uses a proportional temperature control algorithm to maintain temperature stability. This will hold the bath temperature to within 0.5°C of the temperature set point. There will, however, be a small variation in temperature vertically through the bath. Experience will show the best temperature to be set for any given requirement and ambient temperature.

Product Specifications

Display Resolution:   0.1°C
Temperature Accuracy:   +/- .5°C
*Temperature Range:   +8°C to 0°C
Voltage Requirements:   230V 50 Hz or 115V 60 Hz
Power Rating:   60W
Inlet Fuse Rating:   2A

*Note that the actual temperatures achievable will be dependent upon the solutions used and local temperature conditions.

2. Integrally Mounted Cold Light Source

Model 7000-2-1

Product Description

To facilitate the careful slicing operation, it is most important that the progress of the blade through the tissue be clearly observed. This observation is used to ensure control of the speed of advance and of the oscillation of the blade. Two elements are required for clear observation, light and magnification.

Product Features

      • Ability to direct the light where needed with two flexible fiber optic light guides
      • Maintains temperature stability of the tissue or surrounding a.c.s.f.
      • Light Intensity is adjustable by a rotary dial knob
      • 20W/12V Halogen lamp
      • 2000 hour life approx
      • Fan and electronic power supply
      • Adjustable with potentiometer
      • Twin 500 mm fiber optic light guides

3. Integrally Mounted Magnifying Glass

Model 7000-1-3

Product Description

For general global slices a magnifying glass offering approx 2x magnification is sufficient. However, if specific loci are under study then an inspection microscope is desirable. The binocular inspection microscope with 10x-40x magnification, a zoom of 1x-4x and a working distance of 80 mm is ideal for the task.

Product Features

      • 115 mm Diameter
      • 305 mm Focal Length
      • 2x Magnification

4. Integrally Mounted Inspection Microscope

Model 7000-1-1
(x20-x40)

5. Integrally Mounted Inspection Microscope

Model 7000-1-2
(x5 and x10)

Consumables

1. Ceramic Blades

Model 7550-1-C

Product Description

The new blade holder design is now included with all Integraslice and Vibroslice instruments. 7550-1-C and 7550-1-SS are designed for new Integraslice instruments.

Product Features

      • Made from ultra hard zirconium
      • Honed by a process of "lapping" both sides of the single bevel to micron flatness
      • Great rigidity, hence a straight cutting edge
      • Prolonged slice life, especially in the most difficult of tissues such as young brain or very old brain
      • Inert and impervious to corrosion
      • Initial higher cost is offset by its longevity
      • Fits into a special blade holder now standard on both Integraslice and Vibroslice instruments

2. Stainless Steel Blades

Model 7550-1-SS

Product Features

      • Double beveled on both faces, honed to an acute cutting edge and hardened to 56 Rockwell.
      • Nevertheless, stainless steel is relatively soft and these blades are usually used once or at a maximum changed every day.
      • These blades are for use in specially designed blade holders for Vibroslice instruments manufactured prior to 2003.

Blade Specifications

  7550-1-C 7550-1-SS
Material Ceramic Stainless Steel
Dimensions 38 mm x 7 mm x 0.5 mm 38 mm x 9 mm x 0.5 mm
Geometry Single sided Double sided
Bevel Single bevel Double bevel
Honing Lapped edge Ground edge
Packaging 5 per pack 50 per pack
Cat # Description Pack Price Quote Quantity
Cat #: 7610-A Description: Controlled Tissue Bath Pack: Each Price: $2,995.00 Add to Quote:
Cat #: 7000-1-3 Description: Magnifying Glass, Mounted to Machine Pack: Each Price: $482.00 Add to Quote:
Cat #: 7000-1-2 Description: Inspection Microscope fixed x5 & x10 Pack: Each Price: $997.00 Add to Quote:
Cat #: 7000-1-1 Description: Inspection Microscope zoom x10-40 Pack: Each Price: $1,495.00 Add to Quote:
Cat #: 7000-2-1 Description: Cold Light Source Pack: Each Price: $1,237.00 Add to Quote:
Cat #: 7000-3-2 Description: Stainless steel inner specimen bath Pack: Each Price: $495.00 Add to Quote:
Cat #: 7000-4-1 Description: Specimen Mount Only Pack: Each Price: $127.00 Add to Quote:
Cat #: 7000-4-2 Description: Tilting Tissue Mount Pack: Each Price: $225.00 Add to Quote:
Cat #: 7000-5-3 Description: Blade holder- special angle Pack: Each Price: $795.00 Add to Quote:
Cat #: 7000-6-1 Description: Opti-Cal' Z-axis measurement, traceable Pack: Each Price: $1,235.00 Add to Quote:
Cat #: 7000-7-1 Description: Blade handling tool Pack: Each Price: $46.00 Add to Quote:
Cat #: 7000-50-1 Description: Tool kit Pack: Each Price: $60.00 Add to Quote:
Cat #: 7000-60-1 Description: Transit crate, packaging expansion bags Pack: Each Price: $325.00 Add to Quote:
Cat #: 7550-1-C Description: CERAMIC BLADES 5/PK Pack: 5 Pack Price: $395.00 Add to Quote:
Cat #: 7550-1-SS Description: Stainless Steel Blades for Vibrating Microtome Pack: 50 Pack Price: $175.00 Add to Quote:
Cat #: 7000-3-1 Description: Tissue Bath Mount Pack: Each Price: $675.00 Add to Quote:
Cat #: EMS7000smz-2 Description: 7000smz-2 Programable Vibrating Microtome Pack: Each Price: $15,995.00 Add to Quote: