CryO3 Microtome/Cryostat

Innovative Technology
Choose flexibility – Preserve tissue and cut complete sections

The Tissue-Tek Cryo3 Flex Cryostat is the 3rd generation of the established, reliable and trusted Cryo3 platform for fast sectioning of frozen tissue specimens. It was designed to decrease the time to get complete sections, and in parallel to preserve valuable tissue.

When cutting frozen sections time is of the essence. The Tissue-Tek Cryo3 Flex Cryostat is the first and only cryostat with a precision 3D chuck for precise, rapid, and complete sectioning, preserving valuable tissue.

The Tissue-Tek Cryo3 Flex Cryostat produces superior sections from 1 to 99 µm using a temperature-controlled blade holder and a 3D Precision Chuck to accurately align the block face to the blade, reducing operator's trimming time and preserving specimens. The sturdy chuck retains its position when locked and unlocked, eliminating any time and effort spent realigning the cutting angle.

Users can now leverage the newly developed Anti-Roll Rake that was designed to best mimic the preferred and widely accepted "Brush Technique" to prevent sections from rolling, which in addition, reduces the learning curve of new users making them proficient faster.

For the first time in the history of cryotomy, the advanced online remote diagnostics technology platform, iSupport, has been successfully integrated into the hardware and software of the Cryo3 Flex. This ensures faster instrument support and maximum uptime.

A color touch LCD screen provides a visual display of critical sectioning information, such as current chamber temperature, section thickness, and trimming thickness. The Cryobar offers 12 positions, allowing operators to use the Cryomold method or direct method at the same time. The Cryo+ area of the Cryobar freezes to -50° C, within 5 minutes after activation.

The motorized advance and retract keys allows smooth, precise orientation of the 3D Precision Chuck to the blade holder. The blade holder accommodates Accu-Edge® high and low-profile disposable blades.

The Tissue-Tek Cryo3 Flex D (Disinfection) and DM (Disinfection/Motorized) models offer innovative options like motorized sectioning, ozone disinfection, and a vacuum debris removal system. The Cryo3 now uses a more efficient and patented ozone technology to safely protect operators from pathogens in between weekly decontamination requested by CAP Accreditation Guidelines. The combination of ozone, UV and elevated temperatures provide unprecedented disinfection with a reduction of up to 5 logs for the SV40 virus.


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Safe, integral disinfection and productivity-enhancing features

  • 3D Precision Chuck
  • 1" Travel Distance
  • Utility Tray with Storage Space
  • 2 Optional Ergonomic Handles
  • Ozone Disinfection (D and DM models)
  • Anti-Roll Rake
  • iSupport: Online Report Diagnostics Technology


  • Obtain complete sections faster
  • Retain position when locked and unlocked
  • Quick and precise fine angle adjustments on all 3 axes (XYZ)
  • Cut thicker specimens
  • Store up to 32 specimen holders in the chamber to keep as many blocks frozen for immediate recuts
  • Section more comfortability
  • Help prevent Repetitive Motion Disorder (RMD)
  • Maximize instrument uptime
  • Get instrument support faster
  • Protect yourself from pathogens with an unprecedented disinfection of up to 5 logs for the SV40 virus
  • Prevent sections from rolling


Application Cryotomy
Models #6200 Tissue-Tek Cryo3 Flex Cryostat, Basic
#6201 Tissue-Tek Cryo3 Flex Cryostat, D (Disinfection and Vacuum)
#6202 Tissue-Tek Cryo3 Flex Cryostat, DM (Disinfection, Vacuum and Motorized Sectioning)
Dimensions (WxDxH) 25.8 x 29.7 x 47.0 inches (65.4 x 75.4 x 119.4 cm)
With Handle Extended:
28.7 x 29.7 x 47.0 inches (73.0 x 75.4 x 119.4 cm)
Weight 373 lbs (169.2 kg)
Power Requirement 115/120 VAC, 50/60 Hz, 11 Amps
The instrument must be connected to an electrical outlet which supplies the proper voltage (within ± 10%)
Data Interface USB and LAN
Operating Conditions 15 to 28°C (59.0 to 82.4°F) maintains a temperature of -35°C
29 to 35°C (84.2 to 95.0°F) maintains a temperature of
Chamber Temperature Set Point Range -35°C to 0°C; Chamber temperature is maintained within ±2°C
Specimen holder sizes Round 26 mm, 36 mm, or 55 mm (all including an alignment mark on the shaft)
Cryo+ Temperature When activated, Cryo+ is 20°C lower than the Chamber temperature within 5 minutes. Lowest Cryo+ temperature is -50°C
Maximum Specimen Thickness 25 mm
Section Thickness Settings 1 to 99 µm in 1 µm steps
Trimming Range 1 to 100 µm in 1 µm steps
Specimen Holder Sizes Round 26 mm, 36 mm, 55 mm (all including an alignment mark on the shaft)
Specimen Orientation ±8° (XY axis), 360° (Z axis)
Chuck 3D Precision Chuck secures the Specimen Holder to the Microtome. Dial indicators show the exact angle of the Chuck. The guide marks show the exact orientation of the Specimen Holders in the Chuck
Travel Range Vertical: 2.5 inches (63.0 mm)
Horizontal: 1.0 inch (25.4 mm)
Defrost Cycle Manual start: Defrosts the Chamber within 30 minutes when activated
Automatic start: Defrosts the Chamber within 1 hour; start time can be set within a 24 hour period
(D and DM only)
Manual start: Two Ozone Disinfections cycles are available:
  • Long: approximately 135 minutes (warm)
  • Short: approximately 45 minutes (cold)
Automatic start: Always performs a Long Disinfection Cycle. Start time can be set within a 24 hour period
Cat # Description Pack Price Quote Quantity
Cat #: 62548 Description: Tissue-Tek® Cryo3 Flex Cryostat, Basic Pack: Each Price: $40,751.50 Add to Quote:
Cat #: 62548-D Description: Tissue-Tek® Cryo3 Flex Cryostat, D Pack: Each Price: $45,474.00 Add to Quote:
Cat #: 62548-DM Description: Tissue-Tek® Cryo3 Flex Cryostat, Dm Pack: Each Price: $48,972.00 Add to Quote: