For easily handling and mounting of section ribbons. The mounting of the manipulator is easy. The original plate on the cryochamber is removed, the manipulator is fixed in 5 minutes.
Applications
- Frozen hydrated biological samples (CEMOVIS)
- Room temperature sectioning of water sensitive samples
- Dry resin sectioning of biological samples for chemical analysis
- Dry sectioning of industrial samples such as polymers
Functionality
- The left manipulator moves a conductive fibre on which the sections are attached by electrostatic force; the growing ribbon is guided.
- The right manipulator guides the grid very precisely below the section ribbon, the sections are attached to the carbon film with electrostatic force.
Compatibility
- Leica cryochambers FC6 and FC7 equipped with a Crion ionizer/charger
- adaption on other cryochambers upon request
References
The cryosectioning approach is described by Struder et al.:
Struder, D., Klein A., Iacovache, I, Gnaegi, H., and Zuber B. (2014). A new tool based on two micromanipulators facilitates the handling of ultrathin cryosection ribbons. Journal of Structural Biology, 185, 125-128.
What services can we offer you?
- Technical assistance in all fields of ultramicrotomy.
- Free sectioning tests for all types of samples.
- Make use of our many years of experience in perfecting our knives.
- With a telephone call, an e-mail or a fax message we can inform you of any details you require.