Fluoro-Gel III Mounting Medium is similar to Fluoro-Gel, however, Fluoro-Gel III with the addition of PI (Propidium Iodide) which is a counterstain for DNA, makes this a unique mounting medium, which prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa Fluoro 488, Alexa Fluoro 594, Green Fluorescent protein (GFP), Tetramethylyrhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC). The fluorescence is retained during prolonged storage at 4°C in the dark. This medium does not contain Phynylenediamine, which destroys immunofluoresccence of Cy dyes, RP-E, PC, and APC. This product is to be used in in situ hybridization techniques or other method where fluorescence of DNA staining is required. PI excites at 535 nm, emits at 615 nm, producing a red fluorescence. RNA is also stained with PI.
Application: Immunofluorescence, confocal microscope.