Immersion Oil Type LDF

Immersion Oils For Fluorescence Microscopy

After years of development and field testing, we now have available two new PCB-Free Immersion Oils for Fluorescence Microscopy.

* Immersion Oil Type HF

Type DF has been discontinued by Cargille. As an alternative we are offering either a new Immersion Oil called HF which would provide low fluorescence and the correct optical properties or you can use our FF which has virtually no fluorescence but slightly less than ideal optical properties.

* Immersion Oil Type FF

Type FF has virtually no background fluorescence which makes it ideal for observing low levels of fluorescence. It is highly stable, water white, and non-hydroscopic. Type FF can be substituted for all other fluorescence microscopy immersion oils in nearly all fluorescence applications.

* Immersion Oil Type LDF

Extremely low fluorescence is achieved by Type LDF. It is the replacement of choice for the DF which is no longer being manufactured. The LDF possesses the highest resolution of the oils for fluorescence applications.

* Immersion Oil Type 37LDF

Low fluorescence immersion oil for use at 37°C. Distinct odor.

Refractive Index @ 37°C
F Line (486.1 nm) - 1.5239
E Line (546.1 nm) - 1.5181
D Line (589.3 nm) - 1.5150
C Line (656.3 nm) - 1.5115
Viscosity: 1250 cSt ± 10% @ 37°C (High)

OPTICAL & PHYSICAL PROPERTIES

Immersion Oil Type LDF Type FF Type HF
Refractive Index @ 23°C:
  F Line (4861 A) 1.5239 1.4850 1.5234
  E Line (5461 A) 1.5181 1.4811 1.5180
  D Line (5893 A) 1.5150 1.4790 1.5150
  C Line (6563 A) 1.5115 1.4766 1.5117
Dispersion:  
  nF-nC 0.0125 0.0084 0.0118
  Abbe VD 41.3 57.0 43.8
  Abbe Ve 41.0 56.5 43.6
Temperature Coefficient
(15-35°C)
-0.00036 -0.00037 -0.00038
Stability (change in nD25°C after 24 hrs. @ temp.)
  at 60°C: -0.0004 0 +0.0001
  at 100°C: -0.0043 0 +0.0006
Fluorescence (1):      
  Short UV: very low none very low
  Long UV: very low none very low
Color: (Gardner) <1 <1 <1
Viscosity: +/-10% @ 23°C (centistokes) 500
medium
170
low
700
medium
Density:      
  @ 23°C g/cc: 0.984 0.877 .9306
  (US) lb/gal 8.21 7.32 7.77
Cloud Point: <-14°C <-6°C <2°C
Flash Point: 171°F 420°F >340°F
Neutralization No.: (mg KOH/gm) <0.01 max 0.01 max 0.03 max

*Relative to Cedarwood Oil

Products specifications
Attribute name Attribute value
Hazard Hazard - Requires an additional shipping cost.
Storage Cat # Description MSDS Pack Price Quote Quantity
Storage:RT Cat #: 16919 Description: Immersion Oil Type LDF Pack: 1 oz Price: $16.00 Add to Quote:
Storage:RT Cat #: 16919-01 Description: Immersion Oil Type LDF Pack: 4 oz Price: $36.25 Add to Quote:
Storage:RT Cat #: 16919-16 Description: Immersion Oil Type LDF Pack: 16 oz Price: $75.00 Add to Quote:
Storage:RT Cat #: 16919-12 Description: Immersion Oil Type 37 LDF Pack: Each Price: $80.00 Add to Quote:
Storage:RT Cat #: 16919-13 Description: Immersion Oil Type 37 LDF Pack: Each Price: $34.00 Add to Quote:
Storage:RT Cat #: 16919-14 Description: Immersion Oil Type 37 LDF Pack: Each Price: $16.00 Add to Quote:
Storage:RT Cat #: 16919-15 Description: Immersion Oil Type 37 LDF Pack: Each Price: $11.00 Add to Quote: