Methenamine Silver Method for Argentaffin Cells
For the staining of argentaffin granules of enterochromaffin cells.
Stain results:
| Argentaffin Cells: |
|
Black |
| Coarse connective tissue of the submucosa: |
|
Variable amount of
blackening by 3–31 ⁄2 hr. of impregnation |
Granules of eosinophil leukocytes, nuclei,
smooth muscle & surface epithelium: |
|
show additional blackening
after incubation beyond 3–31 ⁄2 hr. |
| Granules of mast cells: |
|
Remain red after nuclei &
reticulum are blackened |
References:
- Clark, G.: Staining Procedures, Williams and Wilkins Co., Baltimore, 3rd Ed., c. 1973, p. 161.
- Gomori, G., Arch. Path., 45:48, 1948.
- Burtner, H.J. and Lillie, R.D., Stain Techn., 24:225-7, 1949.