The Ready-to-Use Incubation Buffer Solutions are applicable in all types of immuno(cyto)chemical procedures, not only in ImmunoGold Silver Staining, and also in immunofluorescence and immuno-enymatic techniques in microscopic and macroscopic applicatio.
AURION BSA-c™ is an acetylated and (partly) linearized form of Bovine Serum Albumin, having an increased net negative charge by acetylation of amino groups of basic amino acids. AURION BSA-c™ will improve signal-to-noise ratios independent of the immunodetection system being used.
TWEEN-20® is a non-ionic surfactant, a detergent with hydrophobic alkyl side chains. When other hydrophobic components (proteins, antibodies, gold conjugates etc.) are present these components are likely to get embedded in micellar structures. One of the main effects is the suppression of hydrophobic (background) interactions. It acts as a surfactant and thus will enhance reagent spreading in (automated) immuno incubation procedures.
Features
- For immuno histo- and cytochemistry, bio-assays, immunogold (silver) stain-ing, immunofluorescent applications, and immuno-enzymatic methods
- Ready-to-use
- Long-term stability
PBS (10mM Phosphate buffer, 150mM NaCl) + 0.1% BSA-c™ + 0.1% TWEEN-20® + 15mM NaN3, pH 7.4. A protein block step may be omitted from the immuno incubation procedure (See protocol no. 2 in Technical Tips and the Important Note).