Dane's Method for Prekeratin, Keratin, and Mucin

Keratin stain method for the staining of keratin, prekeratin, acid mucopolysaccharides and nuclei.

Stain results:

Acid Mucopolysaccharides:   Blue
Prekeratin and Keranin:   Orange to Red
Nuclei:   Brown

References:

Dane, E.T., and Herman, D.L.: Stain Technology, 38:97, 1963.
AFIP Manual of Histological Staining Methods, 3rd Ed., Ed L. Luna: New York: McGraw Hill Publications, c 1968, p.83.

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