PP3010 Cryo-SEM/Cryo-FIB/SEM Preparation System

PP3010 Cryo-SEM/Cryo-FIB/SEM Preparation System

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Quick Overview

The PP3010 is a highly automated, easy to use, column-mounted, gas-cooled cryo preparation system suitable for most makes and models of SEM, FE-SEM and FIB/SEM.

Key Features

  • High resolution performance on SEM, FE-SEM and FIB/SEMs
  • Totally gas cooled, including cryo preparation chamber – no boiling nitrogen on the SEM
  • Efficient cooling (down to at least -190°C)
  • 24 hours plus run times on one fill of LN2 are typical – allowing unattended overnight operation (at typical operating temperatures)
  • Large recipe driven touch-screen interface
  • Automated sublimation, coating and system start up
  • Superb specimen visibility (including preparation chamber CCD camera)
  • Fully compatible with SEM beam deceleration/stage bias modes up to 5kV
  • Off column cooling and pumping systems – minimum mass on the SEM
  • On-screen data logging and diagnostics
  • Pumped storage of the cryo transfer device
  • Prepdek® workstation – self contained work area, extra bench space not required
  • Cryo workflow options
  • Specialist support and three-year warranty

Product Description

The PP3010 is a highly automated, easy-to-use, column-mounted, gas-cooled cryo preparation system suitable for most makes and models of SEM, FE-SEM and FIB/SEM.

The PP3010 has all the facilities needed to rapidly freeze, process and transfer specimens. The cryo preparation chamber is turbomolecular pumped and includes tools for cold fracturing, controlled automatic sublimation and sputter coating. After processing, the specimen is transferred from the cryo preparation chamber onto a highly stable SEM cold stage for observation. Cold trapping in the cryo preparation chamber and SEM chamber ensures the whole process is frost-free. Specimen process times are typically between five and ten minutes.

Mounting, Freezing and Transferring Specimens – Easy with the Prepdek® Workstation

The PP3010 Prepdek® workstation is fitted with a slushy nitrogen freezing station, connected to the pumping system. Rapid freezing reduces ice crystal damage and results in improved specimen preservation. For handling pre-frozen material the Prepdek® freezing system allows specimens that have been frozen by alternative freezing methods (or stored field specimens) to be manipulated – in or just above liquid nitrogen – and then transferred under vacuum into the PP3010 preparation chamber for subsequent processing and observation.

Additionally the TEM Prep Slusher and Glove Box Interface/Airlock options allow workflow amongst a range of other platforms, including cryo-TEM, cryoultramicrome, XPS and glove box.

Cryo Transfer Device – Including Vacuum Storage

The vacuum transfer device is compact (fits easily into one hand), reliably vacuum-tight and has a bayonet connection to the specimen shuttle to ensure rapid pick up and transfer.

Set into the Prepdek® work surface is a pumped storage tube for the cryo transfer device (see Prepdek® workstation section below).

Specimen Stubs, Shuttles

The PP3010 is supplied with universal 10 mm specimen stubs with surface slots, holes and a flat area – useful for most specimen types. Blank and slotted stubs are also included. In addition a range of optional holders is available, including shuttles for large specimens and top-loading holders for high pressure freezing, TEM Autogrids (for cryo-FIB/SEM applications) and clamping shuttles for hard specimens.

Cryo Preparation Chamber

The cryo preparation chamber is connected directly to the microscope and includes a highly efficient nitrogen gas cold stage, extensive cold trapping and facilities to fracture, sublimate and sputter coat specimens. The chamber is fitted with two fully integrated and interlocked gate valves. The outer load-lock valve includes a pumped airlock which accepts the cryo transfer device – the inner SEM valve ensures rapid high-vacuum to high-vacuum specimen exchange.

Highly Efficient Gas Cooled Stage and Cold Traps

At the heart of the cryo preparation chamber is a nitrogen gas cooled specimen stage. The stage has a dovetail fitting to accept a cryo shuttle and can be precisely controlled over a temperature range from 100°C to -190°C or lower. Large gas cooled cold traps located above, below and behind the specimen stage ensure clean, high vacuum conditions in the chamber.

Both the cold stage and cold traps are cooled with the fully integrated CHE3010 off-column cooling system (see below), which at normal operating temperatures give typical hold times of at up to 24 hours between fills (provided the nitrogen gas is dry).

High Visibility – Plus CCD Camera

There is superb visibility into the preparation chamber. In addition to the large front window (75 x 150 mm), there are two top viewing ports. The chamber is lit by three LEDs and a CCD camera allows the specimen cold stage area to be viewed on the control screen and the images saved.

An optional stereo microscope can also be fitted to the cryo preparation chamber

Cold Fracturing

Twin fracturing tools manipulators (actively cooled) are available and allow a range of specimen types to be cold fractured.

The PP3010 is fitted as standard with a front mounted fracturing and manipulation device. The ball-jointed mount offers flexible movement of the blade which can be used both as a surface pick (probe) and a fracturing knife.

An optional micrometer-advanced fracturing tool with rigid blade is available, in addition to the standard front-mounted tool.

Fractured fragments are captured in the large cold trap located below the specimen stage.

Automatic Sublimation and Sputtering

Sublimation temperatures and times can be preset and stored for easy retrieval. The process is fully automatic and graphically displayed on the control screen, showing the actual verses the predicted temperature curves.

The high resolution sputter coater is based on the market leading series of bench top coaters. The coating system will give fine grain films essential for FE-SEM applications. A platinum target is fitted as standard – optional metals include gold, gold/palladium, chromium and iridium. An optional fully integrated carbon fiber evaporation head can also be fitted.

An optional terminating film thickness monitor is available.

Turbomolecular Pumping – High Vacuum Performance

The preparation chamber is pumped by a remotely-positioned 70 L/s turbomolecular pumping system. Typical preparation chamber vacuums when cold are in the region of 10-7mbar or better. Positioning the turbomolecular pump away from the SEM ensures total elimination of mechanical vibration and has the advantage of significantly reducing the total cryo system mass connected to the SEM. A vacuum buffer tank (remotely located in the Prepdek®) is automatically pumped when required. The pumping system is connected to the preparation chamber by flexible stainless-steel bellows, which allows flexible positioning of the pumping system.

A 5 m3/hr rotary vacuum pump is required to "back" the turbomolecular pump and for slushing and rough pumping operations. The rotary pump can be located up to five meters from the system, allowing remote location if required. Dry pumping alternatives are available – see Ordering Information.

Prepdek® Workstation

The Prepdek® workstation has been designed to allow specimen mounting, freezing (plus pre-frozen specimen manipulation) and transfer device storage on one ergonomically designed work surface. The control electronics are mounted in a sealed but accessible cabinet beneath the Prepdek®.

Set into the work surface is a pumped storage tube which allows the cryo transfer device to be stored under vacuum conditions when not in use.

Panel PC Touch Screen User Interface

The PP3010 is controlled using a large touch screen panel PC, mounted on the Prepdek® workstation. User-defined 'recipes' can be entered and stored for instant future access. The screen can be set to suit operator preferences; for example, vacuum measurement can be displayed in millibar, Pascal or Torr.

Many of the key steps in the specimen preparation process are automated (system set up, gas flow control, sublimation and sputter coating).

SEM Cold Stage, Cold Trap and Cooling System

A highly stable, thermally isolated, nitrogen gas-cooled stage attaches to the SEM stage. The SEM stage and cold trap are cooled by separate cold gas circuits – both capable of reaching temperatures of -190°C or lower. This configuration allows the operator to select stage and cold trap temperatures that are optimized for specific specimens. For example, for some non-biological materials, it is useful to hold the specimen at very low temperatures – for example, a cold stage temperature of -175°C. This is possible with the PP3010, as cold trap temperatures of -190°C or lower can be selected, but not possible with conduction cooled systems. The SEM cold stage has a temperature range of down to -190°C and a temperature stability of < 0.5°C.

Compatibility with SEM Stage Bias Mode

The PP3010 cold stage is fully compatible with SEM stage bias/beam deceleration modes of up to 5kV.

CHE3010 Off-Column Cooling

The CHE3010 is a fully integrated, remotely mounted cooling system which comes as standard with every PP3010. The CHE3010 is used to cool the SEM stage, SEM cold trap and cryo preparation chamber cold stage and cold traps and will typically reach temperatures down to -190°C or lower.

The CHE3010 is remotely positioned (typically on the floor behind the microscope) and at normal operating temperatures can run for up to 24 hours between fills. This greatly simplifies the cryo process (no more checking on dewar status and topping off), but also allows overnight, unattended operation – particularly useful for some automated FIB/SEM "slice and view" protocols.

Single Port Interface to the SEM or FIB/SEM

Where SEM geometry allows, both the cryo preparation chamber and the SEM cooling system can be fitted to a single chamber port (the minimum port diameter is 38 mm). This gives a tidy installation and frees up a valuable chamber port.

Options and Accessories

CHE3010 Rapid Warming Tower

This device is meant to facilitate a rapid warming of the CHE3010 (Cryo-Heat Exchanger) associated with the PP3010 Cryo-SEM preparation system. The Rapid Warming Tower in conjunction with a 1 liter/min Nitrogen gas flow rate dramatically reduces the lag time before the SEM or Prep chamber components return to room temperature so venting can occur. A process that normally takes hours can be completed in minutes, allowing maintenance or reconfiguring the SEM back to room temperature operation. Accepts many common, locally obtained hair dryers. (Hair Dryer not included).

Specimen shuttles and stubs

The PP3010 is supplied with a selection of holders, and a range of additional specimen shuttles and stubs is also available. (See Ordering Information for details).

Carbon Evaporation and Film Thickness Monitor

A carbon evaporation attachment and a terminating film thickness monitor can be fitted. Both are fully integrated (no external control boxes required).

Cryo Rotate Stage

Offering 360° rotation, the innovative Cryo Rotate Stage allows for ion milling and observation/analysis of samples at cryogenic temperatures down to -160 °C. 
The unique Cryo Rotate Stage facilitates cryo-FIB lamella preparation, cryo-FIB lift out and cryo-tomography. The Cryo Rotate Stage is compatible with most makes and models of SEM and FIB/SEM. 

Pressurized LN2 Dewar

The PP7450/60L is a highly recommended option that generates dry nitrogen gas used for cooling the SEM cold stage & cold trap and cryo preparation chamber and cold shields. In addition, LN2 can be decanted for slushing (freezing). During normal operation the PP7450/60L will generate dry nitrogen gas for up to eight days usage.

If the PP7450/60L is not included, appropriate, locally sourced, nitrogen gas cylinders can be used. It is important to ensure that it has low moisture content – if in doubt, please contact us.

Cryoflow Work Options

The airlock is connected to a glove box using a generic NW fitting and for most applications requires a suitable pumping system (rotary pump or turbomolecular plus diaphragm pumping system).

The airlock is designed to accept the PP3010 vacuum transfer device.

For full details of this and other accessories please see Ordering Information.

Pre-Frozen Sample Loading Jig for PP3010

This loading jig allows you to comfortably load pre-frozen samples, ie: HPF or Rivets, into the PP3010 specimen shuttles (not included). The loaded shuttles can then be transferred to the PP3010 slushing pot on the Prepdek®. Includes the LN2 container, Lexan cover, loading jig, and a short transfer rod.

TEM Prep Slusher Option

Offers an easy and convenient way of transporting pre-frozen specimens to and from the PP3010

  • Conveniently locates into the Prepdek® workstation of the PP3010
  • Freely transportable, e.g. between high pressure freezer, cryoultramicrotome and the PP3010
  • Ideal for loading/unloading TEM grids and grid holders
  • Tilting holder for cryo shuttles (holders). Allows the easy transfer of specimens from external freezers (e.g. high pressure, jet, slam etc)
  • Option for PP3010 and previous PP3000 and PP2000/PP2000 models

Additional Information

Specifications

Cryo Preparation Chamber (Column-Mounted)
  Standard?
Gas-cooled preparation chamber with a twenty-four hour run time between fills Yes
Two integral gate valves (loading and SEM) with appropriate electrical interlocks. Yes
Variable temperature gas-cooled specimen stage Yes
Large cold shield above, below, behind the cold stage Yes
Robust micrometer-fed fracturing knife (actively cooled) Option
Side-mounted surface knife/probe (actively cooled). A range of scalpel blades can be fitted to suit different specimen requirements Yes
Automatic sublimation (controlled and viewed on the touch screen) Yes
Fully automatic, high resolution sputter coater with platinum (Pt) target. (Other targets, including gold (Au), gold/palladium (Au/Pd), chromium (Cr) and iridium (Ir), are available as options.) Sputtering controlled and viewed on the user touch screen. Yes
Carbon fiber evaporation head and power supply Option
Large front viewing window (150 x 78 mm) plus top viewing ports Yes
Preparation chamber camera (CCD) Yes
Vacuum transfer device Yes
Chamber illumination – three LEDs Yes
Pumping System and Controls
Remotely-mounted turbomolecular-pumping system (70L/s). Includes: vacuum buffer tank, vacuum valves and stainless-steel bellows connection to the preparation chamber. Typical preparation chamber vacuum when cold: 10-7mbar. Yes
Single 50L/m rotary pump required Order separately
SEM Cooling Dewar, SEM Cold Stage and Cold Trap (Anticontaminator)
Gas-cooled nitrogen cold stage assembly (-190°C). Temperature stability of >0.5°C Yes
Separate gas-cooling circuits for SEM stage and SEM anti-contaminator Yes
21L capacity, off-column cooling dewar with run time between fills of up to 24 hours Yes
SEM CCD camera – fitted when space allows Yes
LED lighting (interlocked) Yes
System Control and Specimen Handling
Control via a color user touch screen monitor (15") mounted on the Prepdek®
  • Multi-ability user interface screen
  • Quick, easy overview of system status
  • User-definable 'recipes' can be stored
  • Quick access to videos outlining preparation techniques and system maintenance
  • Fully automatic sputtering
  • Automatic sublimation
  • Quick, easy overview of system status
  • CCD camera image of preparation chamber and microscope chamber
Yes
Twin liquid nitrogen slushing and specimen handling system – ideal for handling pre-frozen specimens. Mounted on the Prepdek® Yes
System electronics stored in a ventilated, sealed unit under the Prepdek® Yes
Specimen shuttles (x2). E7449-9 multi-specimen stubs (pack of 10) and E7402 blank aluminum (Al) stubs (pack of 10). Other shuttles and stubs available – see Ordering Information Yes
Specimen Shuttles and Stubs (Others available – see Ordering Information)
  • (2) Specimen shuttles (to hold 10 mm diameter cryo stubs)
  • Blank 10 mm stubs – pack of 10
  • Multi-stubs 7 mm high (with holes and slots) – pack of 5
  • Multi-stubs 5 mm high (with holes and slots) – pack of 5
  • Dovetail holder shuttle
  • Brass rivets for fracturing liquids – pack of 100
  • Copper (Cu) stub with 3 mm x 3 mm slot – pack of 5
  • Copper (Cu) stub with 1 mm x 3 mm slot – pack of 5
Yes
Installation and Training
Installation and training at the customer site Contact EMS
Support and Other Information
Comprehensive start-up kit with key spares Yes
Three-year warranty Yes
SEM column interfaces and SEM stage adapter (tailored to each microscope) Yes
Some Options and Accessories (see Ordering Information for full list)
Terminating film thickness monitor (FTM) Option
Self-pressurizing LN2 dewar and regulator (for storage and venting) Option
Carbon fiber evaporation head Option
Wide range of specimen holders and specimen stubs Option

Ordering Information

For a full quotation, including on-site installation and customer training, please contact us.

For a full quotation, including on-site installation and customer training, please contact us.

Cat # Description Pack Price Quote Quantity
Cat #: PP3010T Description: PP3010 Cryo-SEM/Cryo-FIB/SEM Preparation System Pack: Each Price: $0.00 Add to Quote:

Pumping
PP3010 has an integrated turbomolecular pump, but also requires a 13034 rotary pump or 20063 oil-free scroll pump

Cat # Description Pack Price Quote Quantity
Cat #: 20063 Description: Edward NXDS6i oil-free scroll pump Pack: Each Price: $0.00 Add to Quote:

Shipping

Cat # Description Pack Price Quote Quantity
Cat #: 13064 Description: Wooden Crate And Packing For Export Pack: Each Price: $0.00 Add to Quote:

Installation and Training

Cat # Description Pack Price Quote Quantity
Cat #: PP3010T-I Description: Four Day On Site Installation And Customer Training Pack: Each Price: $0.00 Add to Quote:
Cat #: PP3010T-T Description: Travel From Pa To Customer Site, Including Hotels Pack: Each Price: $0.00 Add to Quote:

PP3010 Options and Accessories

Cat # Description Pack Price Quote Quantity
Cat #: 31354 Description: Cryo Rotate Stage Pack: Each Price: $0.00 Add to Quote:
Cat #: PP3050-SLJ Description: Pre-Frozen Sample Loading Jig For PP 3010 Pack: Each Price: $0.00 Add to Quote:
Cat #: PP7450 Description: Pressurized Dewar (60L) For Ln2 Storage And Venting Gas Supply Pack: Each Price: $0.00 Add to Quote:
Cat #: 10996 Description: Glove Box Valve/Airlock Interface Pack: Each Price: $0.00 Add to Quote:
Cat #: 10997 Description: Tem Prep Slusher Pack: Each Price: $0.00 Add to Quote:
Cat #: 13297 Description: Sircal Nitrogen Gas Dryer 220-240V Pack: Each Price: $0.00 Add to Quote:
Cat #: 13298 Description: Sircal Nitrogen Gas Dryer 100-110V Pack: Each Price: $0.00 Add to Quote:
Cat #: PP7424 Description: Binocular Stereo Microscope With Fitting To The PP3010T Pack: Each Price: $0.00 Add to Quote:
Cat #: 20996 Description: Carbon Fiber Evaporation Head And Power Supply Pack: Each Price: $0.00 Add to Quote:
Cat #: 12147 Description: Terminating film thicknesss monitor (FTM) Pack: Each Price: $0.00 Add to Quote:
Cat #: 12145 Description: Micrometer Controlled Fracturing Steel Knife Pack: Each Price: $0.00 Add to Quote:
Cat #: PP3050-RWT Description: Che 3010 Rapid Warming Tower Pack: Each Price: $577.50 Add to Quote:
Cat #: 12340-SPARE Description: Cryo Transfer Device (Spare) Pack: Each Price: $0.00 Add to Quote:

Specimen Shuttles (dovetail). Some will accept 10 mm dia.

Cat # Description Pack Price Quote Quantity
Cat #: AL200077B Description: Standard Specimen Shuttle with 10mm Hole for Cryo Stub Pack: Each Price: $0.00 Add to Quote:
Cat #: 12434 Description: Blank Specimen Shuttle for Large Specimens Pack: Each Price: $0.00 Add to Quote:
Cat #: 20718 Description: Blank Specimen Shuttle with Extended Length Pack: Each Price: $0.00 Add to Quote:
Cat #: 20720 Description: Shuttle For Large, Flat Specimens Pack: Each Price: $0.00 Add to Quote:
Cat #: 13524 Description: Shuttle For Clamping Hard, Flat Specimens Pack: Each Price: $0.00 Add to Quote:
Cat #: 12013 Description: Shuttle for Clamping Larger, Flat Specimens Pack: Each Price: $0.00 Add to Quote:
Cat #: 25276-Q Description: Clamping Shuttle for Large, Hard Specimens Pack: Each Price: $0.00 Add to Quote:
Cat #: 10245 Description: Top Loading Freeze-Facture “Balzers” Planchette Holder Shuttle Pack: Each Price: $0.00 Add to Quote:
Cat #: 13054 Description: Shuttle for HPF “Top Hat” (3mm) Specimen Holders Pack: Each Price: $0.00 Add to Quote:
Cat #: 20529 Description: Shuttle for 10mm Stub, Top Loading Pack: Each Price: $0.00 Add to Quote:
Cat #: 13419 Description: Tilt-Rotate Shuttle Pack: Each Price: $0.00 Add to Quote:
Cat #: 20530 Description: Vice-Style Specimen Shuttle, Top Loading Pack: Each Price: $0.00 Add to Quote:
Cat #: 12406 Description: Special Shuttle for Cryo-FIB/SEM of TEM Autogrid™ Pack: Each Price: $0.00 Add to Quote:
Cat #: 12922 Description: Cryo Shuttle for Fei Polara™ Cartridge Pack: Each Price: $0.00 Add to Quote:
Cat #: 13359 Description: Stem Shuttle for 3mm Tem Grid Pack: Each Price: $0.00 Add to Quote:

Specimen holders stubs (10 mm dia). For use with appropriate shuttles

Cat # Description Pack Price Quote Quantity
Cat #: E7433 Description: Specimen Stub for Freeze Fracture Rivets Pack: Each Price: $0.00 Add to Quote:
Cat #: E7402 Description: Specimen Stubs, Blank, Aluminum Pack: 10 Pack Price: $0.00 Add to Quote:
Cat #: E7449-5 Description: Universal Cryo Stubs, 10mm x 7.5mm Pack: 5 Pack Price: $0.00 Add to Quote:
Cat #: 11541 Description: Universal Cryo Stubs, 10mm x 5mm Pack: Each Price: $0.00 Add to Quote:
Cat #: E7403 Description: Specimen Stubs, Blank, Copper Pack: 10 Pack Price: $0.00 Add to Quote:
Cat #: E7405 Description: Clamping Stubs for Thin Flat Specimens Pack: Each Price: $0.00 Add to Quote:
Cat #: E7406 Description: Specimen Stub, 3mm x 3mm Slot, Copper Pack: 5 Pack Price: $0.00 Add to Quote:
Cat #: E7407 Description: Specimen Stub, 1mm x 3mm Slot, Copper Pack: 5 Pack Price: $0.00 Add to Quote:
Cat #: 328116510 Description: Brass Rivets for Fracturing Liquids Pack: Each Price: $0.00 Add to Quote:
Cat #: 10246 Description: Top-Loading Specimen Holder Shuttle Pack: Each Price: $0.00 Add to Quote:
Cat #: 10247 Description: Top Loading Rivet Holder Shuttle, Vice Style Pack: Each Price: $0.00 Add to Quote:
Cat #: E7401 Description: Specimen Shuttle Stub, Spare Pack: Each Price: $0.00 Add to Quote:

Spares Kit

Cat # Description Pack Price Quote Quantity
Cat #: 13061 Description: Two-Years Spare Kit For PP3010T Pack: Each Price: $0.00 Add to Quote: