Ready-to-Use Incubation Buffer Solutions
Based on AURION BSA-c™
For your convenience, Aurion has developed Ready-to-Use Incubation Buffer Solutions with long-term stability, that significantly reduce the risk of background.
The main component is AURION BSA-c™, a unique buffer additive that prevents aspecific binding of antibodies and immunodetection reagents to polycationic sites in the specimen, such as histone proteins in the nucleus.
The Ready-to-Use Incubation Buffer Solutions are applicable in all types of immuno(cyto)chemical procedures, not only in ImmunoGold Silver Staining, and also in immunofluorescence and immuno-enymatic techniques in microscopic and macroscopic applications.
Introduction
The ready-to-use Blocking Solutions and the incubation media additive BSA-c™ are tuned for optimum background prevention and signal-to-noise ratio. In-depth information can be found in the respective product data sheets.
AURION also offers a number of components that allow researchers to formulate blocking, incubation and wash solutions according to the needs of their own experiments. The ready-to-use Blocking Solutions and the incubation media additive BSA-c™ are tuned for optimum background prevention and signal-to-noise ratio. In-depth information can be found in the respective product data sheets.
AURION also offers a number of components that allow researchers to formulate blocking, incubation and wash solutions according to the needs of their own experiments.
Product Description
AURION BSA-c™ is an acetylated and (partly) linearized form of Bovine Serum Albumin, having an increased net negative charge by acetylation of amino groups of basic amino acids. AURION BSA-c™ will improve signal-to-noise ratios independent of the immunodetection system being used.
TWEEN-20® is a non-ionic surfactant, a detergent with hydrophobic alkyl side chains. When other hydrophobic components (proteins, antibodies, gold conjugates etc.) are present these components are likely to get embedded in micellar structures. One of the main effects is the suppression of hydrophobic (background) interactions. It acts as a surfactant and thus will enhance reagent spreading in (automated) immuno incubation procedures
Features
- For immuno histo- and cytochemistry, bio-assays, immunogold (silver) stain-ing, immunofluorescent applications, and immuno-enzymatic methods
- Ready-to-use
- Long-term stability
Ordering Information
AURION ready-to-use incubation buffer solutions are available in two compositions:
EMS Cat. No. 25561
PBS (10mM Phosphate buffer, 150mM NaCl) + 0.1% BSA-c™ + 15mM NaN3, pH 7.4. It is recommended to include a protein block step in the immuno incubation procedure (See protocol no. 1 in Technical Tips).
EMS Cat. No. 25561
PBS (10mM Phosphate buffer, 150mM NaCl) + 0.1% BSA-c™ + 0.1% TWEEN-20® + 15mM NaN3, pH 7.4. A protein block step may be omitted from the immuno incubation procedure (See protocol no. 2 in Technical Tips and the Important Note).
AURION ready-to-use buffer solutions are supplied in 1000 ml quantities.
Technical Tips
Application Instructions
Protocol no. 1
Protocol no. 2