Ultra Small Immunogold Reagents
Significantly higher sensitivity and penetration can be obtained with Ultra Small Immunogold Reagents, which are prepared with subnanometer gold particles. Such particles have far less influence on the adsorbed antibodies or detecting compounds and consequently the reagents behave more as if uncoupled. The smallest possible reagents should always be chosen for those applications where penetration can or should be fully exploited (in pre-embedding labeling, in hydrated specimens like cryosections and in labeling for light microscopy), or where maximum detection levels need to be achieved (low antigen levels). In conjunction with the highly efficient and easy-to-use R-Gent SE-LM and SE-EM silver enhancement reagents, the Ultra Small Immunogold conjugates are the best choice for any application.

Aurion Ultra Small Immunogold Reagents are prepared with subnanometer gold particles. These particles have far less influence on the adsorbed antibodies or detecting molecules, and consequently the conjugates behave as though they are uncoupled. In conjunction with the highly efficient and easy-to-use R-Gent SE-LM and SE-EM silver enhancement reagents, the Ultra Small Immunogold Reagents are the best choice for any application.

Introduction

Reduction of the gold particle size provides Ultra Small Immunogold Reagents with fundamentally different characteristics when compared with conjugates built around larger particles. While the Conventional Immunogold Reagents can be thought of as particles coated with proteins, Ultra Small Immunogold Reagents are proteins coated with one or more gold particles. With this structure, both the overall size of the conjugates, as well as steric hindrance are decreased.

The table below lists a few physical characteristics of gold conjugates.

Gold Conjugate Physical Characteristics

Product Description

AURION Ultra Small Immunogold Reagents contain 60-80 µg of specific protein/ml for IgG conjugates. F(ab')2, Fab and biotinylated albumin conjugates contain equimolar amounts of conjugated protein. The average gold cluster diameter is less than 0.8 nm.

AURION Ultra Small Immunogold Reagents are used in conjunction with AURION R-Gent SE-EM or SE-LM silver enhancement reagents, developed for electron microscopy and light microscopy/immunoblotting respectively.

The reagents are supplied in PBS with 1% Bovine Serum Albumin and 15 mM NaN₃.

The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.

The products are available in two package sizes:

• Regular package: for 1200 grids or 600 slides
• Small package: for 480 grids or 240 slides.

Specificity

Aurion offers the widest range of Ultra Small Immunogold Reagents. The most commonly used reagent types are available as intact IgG, F(ab')2 and Single Fab conjugates. To view the complete list of available reagents click here.

AURION Ultra Small Immunogold Reagents are prepared using the highest quality antibodies or binding agents available. All antibodies are immuno affinity purified and immuno cross-adsorbed to reduce non-specific interactions.

Storage

AURION Immunogold Reagents have a guaranteed shelf life of 18 months from the date of quality control analysis.

The products should be stored at 4-8°C. Freezing is not recommended.

Technical Tip

Ultra Small ImmunoGold Application Instructions
Why Ultra Small Gold Conjugates

Overview   Micrographs   FAQs   Workshops   Custom Labeling   Protocols   Newsletters