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Buffers
All of our ready-to-use buffers are:
- Conveniently packaged.
- Prepared fresh for each order.
- Economical and time-saving.
- Desired pH should be specified when ordering.
The buffer is a vehicle for the fixative and requires certain properties:
- To maintain constant pH during the time of the fixation. The pH of the fixative in EM is adjusted using the buffer system chosen; e.g. range 6.5-8.0.
- The ionic constitution to prevent the extraction of cellular components, or the deposition of precipitates of fixative chemicals.
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Ordering Info
R-UNIVERSAL Epitope Recovery Buffer (10x stock)
The EMS Universal Buffer may be used in any heat-induced epitope recovery system. In the Antigen Retriever (62700 Series), it guarantees the highest rate of success in recovering epitope for any antibody.
| Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Cat #: 62719-10 | Description: R-Universal Epitope Recovery Buffer (10x stock) | MSDS | Pack: 125 mL | Price: $208.00 |
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| Cat #: 62719-20 | Description: R-Universal Epitope Recovery Buffer (10x stock) | MSDS | Pack: 500 mL | Price: $325.00 |
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| Cat #: 62719-30 | Description: T-Universal Epitope Recovery Buffer 10x Stock | MSDS | Pack: 125 mL | Price: $99.00 |
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| Cat #: 62719-35 | Description: T-Universal Epitope Recovery Buffer 10x Stock | MSDS | Pack: 500 mL | Price: $152.00 |
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| Cat #: 62719-40 | Description: U-Universal Epitope Recovery Buffer 10x Stock | MSDS | Pack: 125 mL | Price: $135.00 |
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| Cat #: 62719-45 | Description: U-Universal Epitope Recovery Buffer 10x Stock | MSDS | Pack: 500 mL | Price: $160.00 |
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| Cat #: 62719-50 | Description: L-Universal Epitope Recovery Buffer 10x Stock | MSDS | Pack: 125 mL | Price: $97.00 |
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| Cat #: 62719-55 | Description: L-Universal Epitope Recovery Buffer 10x Stock | MSDS | Pack: 500 mL | Price: $159.00 |
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| Cat #: 62719-60 | Description: H-Universal Epitope Recovery Buffer 10x Stock | MSDS | Pack: 125 mL | Price: $97.00 |
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| Cat #: 62719-65 | Description: H-Universal Epitope Recovery Buffer 10x Stock | MSDS | Pack: 500 mL | Price: $159.00 |
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| Cat #: 62719-70 | Description: Epitope Recovery Set | MSDS | Pack: Set | Price: $545.00 |
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Section Block
A new class of blocking solutions based on chemically modified and fragmented ultra-pure casein. Effectively reduces unwanted binding of primary antibody and conjugates you use to charged surface of the slide and tissue section.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:4-8°C | Cat #: 62710 | Description: Section Block | MSDS | Pack: 50 mL | Price: $154.00 |
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| Storage:4-8°C | Cat #: 62711 | Description: Section Block | MSDS | Pack: 125 mL | Price: $315.00 |
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| Storage:4-8°C | Cat #: 62712 | Description: Section Block | MSDS | Pack: 500 mL | Price: $1,265.00 |
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Sodium Cacodylate Buffer
Prepared from Sodium Cacodylate Trihydrate. Ideal for maintaining a constant pH use during fixation, our buffers are prepared fresh for each order and conveniently packaged.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:2-8°C | Cat #: 11650 | Description: Sodium Cacodylate Buffer, 0.2M, pH 7.4 | MSDS | Pack: 225 mL | Price: $38.50 |
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| Storage:2-8°C | Cat #: 11652 | Description: Sodium Cacodylate Buffer, 0.2M, pH 7.4 | MSDS | Pack: 500 mL | Price: $54.00 |
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| Storage:2-8°C | Cat #: 11653 | Description: Sodium Cacodylate Buffer, 0.2M, pH 7.4 | MSDS | Pack: 1 L | Price: $105.00 |
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| Storage:2-8°C | Cat #: 11653-1G | Description: Sodium Cacodylate Buffer, 0.2M, pH 7.4 | MSDS | Pack: 1 gal | Price: $260.00 |
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| Storage:2-8°C | Cat #: 11654 | Description: Sodium Cacodylate Buffer, 0.4M, pH 7.2 | MSDS | Pack: 500 mL | Price: $78.00 |
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| Storage:2-8°C | Cat #: 11655 | Description: Sodium Cacodylate Buffer, 0.4M, pH 7.2 | MSDS | Pack: 1 L | Price: $140.00 |
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| Storage:2-8°C | Cat #: 11655-1G | Description: Sodium Cacodylate Buffer, 0.4M, pH 7.2 | MSDS | Pack: 1 gal | Price: $260.00 |
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Sorensen's Phosphate Buffer
A Phosphate buffered mixed salt prepared from sodium phosphate monobasic and sodium phosphate dibasic. Osmolarity is 300 mosmols.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:2-8°C | Cat #: 11600-05 | Description: Sorensen's Phosphate Buffer, 0.2M, pH 7.2 | MSDS | Pack: 500 mL | Price: $29.50 |
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| Storage:2-8°C | Cat #: 11600-10 | Description: Sorensons Phosphate Buffer, 0.2M, pH 7.2 | MSDS | Pack: 1 L | Price: $40.00 |
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| Storage:2-8°C | Cat #: 11600-10-4L | Description: Sorensen's Phosphate Buffer, 0.2M, pH 7.2 | MSDS | Pack: 4 L | Price: $135.00 |
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| Storage:2-8°C | Cat #: 11600-10-5G | Description: Sorensen's Phosphate Buffer, 0.2M, pH 7.2 | MSDS | Pack: 5 gal | Price: $350.00 |
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| Storage:2-8°C | Cat #: 11601-10 | Description: Sorensen’s Phosphate Buffer, 0.2M, pH 7.4 | MSDS | Pack: 1 L | Price: $42.00 |
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| Storage:2-8°C | Cat #: 11601-10-4L | Description: Sorensen’s Phosphate Buffer, 0.2M, pH 7.4 | MSDS | Pack: 4 L | Price: $130.00 |
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| Storage:2-8°C | Cat #: 11601-10-5G | Description: Sorensen’s Phosphate Buffer, 0.2M, pH 7.4 | MSDS | Pack: 5 gal | Price: $340.00 |
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| Storage:2-8°C | Cat #: 11682-05 | Description: Sorensen's Phosphate Buffer, 0.15M, pH 7.4 | MSDS | Pack: 500 mL | Price: $35.00 |
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| Storage:2-8°C | Cat #: 11682-10 | Description: Sorensen's Phosphate Buffer, 0.15M, pH 7.4 | MSDS | Pack: 1 L | Price: $42.00 |
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| Storage:2-8°C | Cat #: 11682-10-4L | Description: Sorensen’s Phosphate Buffer, 0.15M, pH 7.4 | MSDS | Pack: 4 L | Price: $140.00 |
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| Storage:2-8°C | Cat #: 11682-10-5G | Description: Sorensen’s Phosphate Buffer, 0.15M, pH 7.4 | Pack: 5 gal | Price: $352.00 |
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Sym-Collidine Buffer Kit
A very stable buffer, used with Osmium tetroxide; provides excellent fixation. Set consists of: 1 amp 5.34ml Sym Collidine and 1 amp 9.0 ml of 2N HCl.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:RT | Cat #: 11500 | Description: Sym-Collidine Buffer Kit | MSDS | Pack: Kit | Price: $41.00 |
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| Storage:RT | Cat #: 11510 | Description: Sym-Collidine Buffer Kit | MSDS | Pack: 5 Pack | Price: $135.00 |
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Sym-Collidine, Reagent
Sym-Collidine is used in combination with osmium tetroxide to provide excellent fixation and stable buffering. pH does not change when distilled water or osmium tetroxide solution is added.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:RT | Cat #: 11520 | Description: Sym-Collidine, Reagent | MSDS | Pack: 100 mL | Price: $216.00 |
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Tris, (Hydroxymethyl) Aminomethane Maleate
Ideal for maintaining a constant pH use during fixation, our buffers are prepared fresh for each order and conveniently packaged. Specify pH when ordering.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:2-8°C | Cat #: 11730 | Description: Tris Maleate Buffer 0.2M pH Range 6.4-8.0 | MSDS | Pack: 250 mL | Price: $30.00 |
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| Storage:2-8°C | Cat #: 11740 | Description: Tris Maleate Buffer 0.2M pH Range 6.4-8.0 | MSDS | Pack: 500 mL | Price: $39.50 |
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| Storage:2-8°C | Cat #: 11740-1L | Description: Tris Maleate Buffer 0.2M pH Range 6.4-8.0 | MSDS | Pack: 1 L | Price: $61.50 |
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| Storage:2-8°C | Cat #: 11740-1G | Description: Tris Maleate Buffer 0.2M pH Range 6.4-8.0 | MSDS | Pack: 1 gal | Price: $225.00 |
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| Storage:RT | Cat #: 11730-05 | Description: Tris Buffer 0.2M pH 8.0 | MSDS | Pack: 250 mL | Price: $30.00 |
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| Storage:RT | Cat #: 11730-06 | Description: Tris Buffer 0.2M pH 8.0 | MSDS | Pack: 500 mL | Price: $39.50 |
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| Storage:RT | Cat #: 11730-06-1L | Description: Tris Buffer 0.2M pH 8.0 | MSDS | Pack: 1 L | Price: $61.50 |
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| Storage:RT | Cat #: 11730-06-1G | Description: Tris Buffer 0.2M pH 8.0 | MSDS | Pack: 1 gal | Price: $225.00 |
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| Storage:2-8°C | Cat #: 11730-08 | Description: Tris Buffered Saline pH 7.4 | MSDS | Pack: 250 mL | Price: $30.00 |
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| Storage:2-8°C | Cat #: 11730-09 | Description: Tris Buffered Saline pH 7.4 | MSDS | Pack: 500 mL | Price: $39.50 |
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| Storage:2-8°C | Cat #: 11730-09-1L | Description: Tris Buffered Saline pH 7.4 | Pack: 1 L | Price: $61.50 |
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| Storage:2-8°C | Cat #: 11730-09-1G | Description: Tris Buffered Saline pH 7.4 | Pack: 1 gal | Price: $225.00 |
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| Storage:2-8°C | Cat #: 11730-12 | Description: Tris-HCL Buffer 1M pH Range 6-8 | MSDS | Pack: 250 mL | Price: $30.00 |
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| Storage:2-8°C | Cat #: 11730-15 | Description: Tris-HCL Buffer 1M pH Range 6-8 | MSDS | Pack: 500 mL | Price: $39.50 |
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| Storage:2-8°C | Cat #: 11730-15-1L | Description: Tris-HCL Buffer 1M pH Range 6-8 | Pack: 1 L | Price: $61.50 |
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| Storage:2-8°C | Cat #: 11730-15-1G | Description: Tris-HCL Buffer 1M pH Range 6-8 | Pack: 1 gal | Price: $225.00 |
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| Storage:2-8°C | Cat #: 11730-20 | Description: Tris HCL Buffer pH Range 7.2-9.0 | MSDS | Pack: 250 mL | Price: $30.00 |
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| Storage:2-8°C | Cat #: 11730-25 | Description: Tris HCL Buffer pH Range 7.2-9.0 | MSDS | Pack: 500 mL | Price: $39.50 |
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| Storage:2-8°C | Cat #: 11730-25-1L | Description: Tris HCL Buffer pH Range 7.2-9.0 | Pack: 1 L | Price: $61.50 |
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| Storage:2-8°C | Cat #: 11730-25-1G | Description: Tris HCL Buffer pH Range 7.2-9.0 | Pack: 1 gal | Price: $225.00 |
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Tris Buffer pH 10.0 (10x)
This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. On FFPE tissue the buffer improves accessibility of antibodies to tissue antigens.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:2-8°C | Cat #: 64142-11 | Description: Tris Buffer pH 10.0 (10x) | MSDS | Pack: 100 mL | Price: $82.00 |
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| Storage:2-8°C | Cat #: 64142-12 | Description: Tris Buffer pH 10.0 (10x) | MSDS | Pack: 1000 mL | Price: $139.00 |
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| Storage:2-8°C | Cat #: 64142-12-1G | Description: Tris Buffer pH 10.0 (10x) | MSDS | Pack: 1 gal | Price: $416.00 |
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Universal Immunoassay Buffer™, 10x
Immunoassay buffer is used as a 1X in immunoassays (immunofluorescence, IHC, ELISA, WB) as a washing buffer. Can be used as a dilution buffer for antibodies. Does not contain carrier proteins, phosphate, sodium azide or mercury.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:RT | Cat #: 25880-01 | Description: Universal Immunoassay Buffer™, 10X | MSDS | Pack: 100 mL | Price: $65.00 |
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| Storage:RT | Cat #: 25880-10 | Description: Universal Immunoassay Buffer™, 10X | MSDS | Pack: 1 L | Price: $182.00 |
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Universal Antibody Dilution Buffer™
For dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. Does not contain mammalian proteins, phosphate, sodium azide, or mercury preservatives. Diluted antibodies can be stored at 2–8 degree C for several weeks.
| Storage | Cat # | Description | MSDS | Pack | Price | Quote | Quantity | |
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| Storage:2-8°C | Cat #: 25885-01 | Description: Universal Antibody Dilution Buffer™ | MSDS | Pack: 100 mL | Price: $66.50 |
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| Storage:2-8°C | Cat #: 25885-05 | Description: Universal Antibody Dilution Buffer™ | MSDS | Pack: 500 mL | Price: $193.00 |
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